YOU Huafang,WU Chenyin,XU Yanjun,et al.Effects of Exogenous GABA on Physiological Indexes of Mycelial of Lentinus edodes Under Different Culture Temperatures[J].Northern Horticulture,2023,(04):111-117.[doi:10.11937/bfyy.20222381]
外源GABA对亚温胁迫马桑菌菌丝生理指标的影响
- Title:
- Effects of Exogenous GABA on Physiological Indexes of Mycelial of Lentinus edodes Under Different Culture Temperatures
- Keywords:
- Lentinus edodes; exogenous GABA; growth inhibition ratio; relief of stress; antioxidant enzymes
- 文献标志码:
- A
- 摘要:
- 以马桑菌（Lentinus edodes）菌株LeM-f为试材，采用外源添加GABA并覆盖赛璐玢膜的平板置于室温和亚温逆境下培养，观测不同浓度γ-氨基丁酸(GABA)对菌丝生长速度、气生菌丝鲜质量、菌丝生长抑制率、显微形态及抗氧化酶活性变化，以期为探索应用GABA对缓解马桑菌菌丝对亚温胁迫的逆境耐受能力、抗性提升效果提供参考依据。结果表明：外源添加适宜浓度GABA能缓解菌丝遭受的亚高、低温胁迫，促进菌丝生长，提高菌丝密度，且增加了前端菌丝的分支率，抗氧化酶（CAT、SOD）活性随外源添加GABA而提升。0.6 mmol?L-1 GABA对亚高温胁迫的缓解效果最佳，生长抑制率低至8.63%，接近室温正常生长水平，气生菌丝产量比亚高温抑制时提升212.47%，分支率提高34.31%，所有效果优于亚低温胁迫。综上所述，外源GABA可效缓解亚高、低温造成的伤害，但亚高温条件下作用最佳，生产中的应用前景很广。
- Abstract:
- Lentinus edodes strain LeM-f was used as test material，plates covered with celluloid membrane were cultured at room temperature and subthermal stress to observe the changes of different concentrations of GABA on the growth rate of mycelia，fresh weight of aerogenic mycelia，inhibition rate of mycelia growth，microscopic morphology and antioxidant enzyme activity,in order to provide reference for exploring the effects of GABA on alleviating the stress tolerance and resistance enhancement effect of Lentinus edodes mycelium to subtemperature stress.The results showed that exogenous addition of appropriate concentration of GABA could alleviate the sub-high and low temperature stress suffered by hyphae，promote the growth of mycelium，increase hyphae density，and increase the branching rate of front-end hyphae，and the activity of antioxidant enzymes (CAT，SOD) increased with the addition of exogenous GABA.GABA of 0.6 mmol?L-1 had the best alleviation effect on sub-high stress with a growth inhibition rate as low as 8.63%，which was close to the normal growth level at room temperature，and the yield of aerial mycelium was increased by 212.47% and the branching rate was increased by 34.31% compared with sub-high temperature inhibition，all effects were better than sub-low temperature stress.In summary，exogenous GABA could effectively alleviate the damage caused by sub-high and low temperature，but it had the best effect under sub-high temperature conditions，and the application prospect in production was very wide.
参考文献/References:
[1]LI L，NA D，HUI Z，et al.The versatile GABA in plants[J].Plant Signaling & Behavior，2021(16):3.[2]RAMOS R R,MARTINEZ F,KNAUF B G.The effects of GABA in plants[J].Cogent Food & Agriculture,2019(5):1670553.[3]AYDIN S，RAHIM N H，MORTEZA S A.Exogenous application of GABA retards cap browning in Agaricus bisporus and its possible mechanism[J].Postharvest Biology and Technology，2021，174:111434.[4]CARILLO P.GABA shunt in durum wheat[J].Frontiers in Plant Science，2018(9):100.[5]WU X，JIA Q，JI S，et al.Gamma-aminobutyric acid (GABA) alleviates salt damage in tomato by modulating Na+uptake，the GAD gene，amino acid synthesis and reactive oxygen species metabolism[J].BMC Plant Biol,2020,20(1):465.[6]LIU C，WANG H，ZHANG X,et al.Activation of the ABA signal pathway mediated by gaba improves the drought resistance of apple seedlings[J].International Journal of Molecular Sciences,2021,22(23):12676.[7]LI Y X，LIU B Y，PENG Y X,et al.Exogenous GABA alleviates alkaline stress in Malus hupehensis by regulating the accumulation of organic acids[J].Scientia Horticulturae,2020,261(9):108982.[8]周莎莎,王刚正,罗义,等.生长素及其类似物增强香菇耐高温性的研究[J].菌物学报,2018,37(12):1723-1730.[9]陆晓晨.根域亚低温胁迫下山定子根系GABA代谢特征及其生理效应研究[D].沈阳:沈阳农业大学,2017.[10]李晓慧,张恩让,何玉安,等.亚高温及外源物质调节下番茄的生理响应[J].江苏农业科学,2013,41(7):135-137.[11]刘秀明.不同食用菌栽培种质对高温胁迫的反应研究[D].北京：中国农业科学院,2017.[12]ORDAZ A,FAVELA E,MENESES M,et al.Hyphal morphology modification in thermal adaptation by the white-rot fungus Fomes sp.EUM1[J].Journal of Basic Microbiology，2012,52(2):167-174.[13]王波,唐利民,熊鹰,等.香菇菌株菌丝和子实体生长耐高温试验研究[J].吉林农业大学学报,2004(2):145-147.[14]刘兵.γ-氨基丁酸和海藻糖对高温胁迫香菇菌丝胞外酶活性的影响[D].太谷:山西农业大学,2017.[15]李玉.烟色离褶伞基内菌丝生长规律及生产中的应用[J].中国食用菌,2009,28(4):18-19.[16]SACHIN K.Complexity of the heat stress response in plants[J].Current Opinion in Plant Biology，2007，10(3)：310-316.[17]郭敬,易美琴,宋波.三种食用菌在温度胁迫下菌丝酶活性的变化研究[J].四川农业科技,2019(5):53-56.[18]李宇星.外源γ-氨基丁酸（GABA）对苹果幼苗碱胁迫的缓解作用及生理机理研究[D].杨凌:西北农林科技大学,2020.[19]李颖,鱼小军,李珍,等.环境胁迫下γ-氨基丁酸对植物生长调控的研究进展[J].草地学报,2022,30(4):835-840.[20]陈凤琼,陈秋森,刘汉林,等.不同外源试剂对菜用大豆低温胁迫的调控效应[J].大豆科学,2022,41(2):165-171.
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备注/Memo
第一作者简介：游华芳（1998-），女，硕士研究生，研究方向为蔬菜（食用菌）生理生态与高效栽培。E-mail：2275818593@qq.com.责任作者：徐彦军(1972-)，男，硕士，教授，现主要从事食用菌教学与科研等工作。E-mail：gdxyj1996@126.com.基金项目：贵州省科技重大专项资助项目（黔科合重大专项[2019]3005）；贵州省科技计划资助项目（黔科合服企[2018]4002-2）。收稿日期：2022-06-09