FU Yue-yue,YANG Hong-yi.Extraction and Elimination of Virus of dsRNA from Pleurotus ostreatus[J].Northern Horticulture,2013,37(12):111-113.
平菇病毒dsRNA的提取及脱除
- Title:
- Extraction and Elimination of Virus of dsRNA from Pleurotus ostreatus
- 文章编号:
- 1001-0009(2013)12-0111-03
- Keywords:
- Pleurotus ostreatus; dsRNA; elimination of virus
- 分类号:
- S 646.1+4
- 文献标志码:
- A
- 摘要:
- 以平菇为试材，采用改进SDS法从平菇组织中提取病毒特异dsRNA，并分别采用化学药剂-菌丝尖端处理和高温-菌丝尖端处理对平菇中的病毒进行了脱毒处理。结果表明：病毒dsRNA特点显示其与国外报道的平菇病毒dsRNA一致。其中高温-菌丝尖端脱除处理的效果最好，可有效脱除平菇病毒；病毒唑、放线菌酮及病毒唑与盐酸吗啉胍联合菌丝尖端脱除处理，可使病毒dsRNA条带减弱。该试验建立了从平菇组织中提取病毒dsRNA的技术体系，可有效对平菇中的病毒进行鉴定。
- Abstract:
- Taking Pleurotus ostreatus as material，the special dsRNAs of virus were extracted from Pleurotus ostreatus with modified SDS method，and elimination of virus in Pleurotus ostreatus was performed with reagent-mycelial tip-cutting and high temperature-mycelial tip-cutting methods.The results showed that the dsRNA was the same as one in foreign studies.The virus could be effectively eliminated with reagent-mycelial tip-cutting method，and the methods，including of ribavirin-mycelial tip-cutting，cycloheximide-mycelial tip-cutting，ribavirin and moroxydine hydrochloride-mycelial tip-cutting，could reducethe quantity of dsRNA of virus.The system of extraction of dsRNA was developed using tissues of Pleurotus ostreatus as material.In addition，the technique could effectively detect virus in Pleurotus ostreatus.
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备注/Memo
第一作者简介：付月月（1986-），女，硕士，研究方向为食用菌学。E-mail:fuyueyue045186@163.com.
责任作者：杨洪一（1978-），男，博士，副教授，现主要从事微生物学研究等工作。E-mail:hyi01@tom.com.
收稿日期：2013-01-16