LIU Zhi-xi,LI Zhi-qiang,WEI Hai-lian,et al. Phylogenetic Analysis of Oyster Mushrooms by ISSR Marker[J].Northern Horticulture,2014,38(12):94-99.
平菇ISSR遗传多样性分析
- Title:
- Phylogenetic Analysis of Oyster Mushrooms by ISSR Marker
- 文章编号:
- 1001-0009(2014)12-0094-06
- Keywords:
- oyster mushroom(Pleurotus); ISSR; phylogenetic
- 分类号:
- S 646.1+4
- 文献标志码:
- A
- 摘要:
- 以53个平菇菌种为试材，采用ISSR标记技术，对采自多地的平菇材料进行遗传多样性检测，以期为平菇优良品种选育和亲缘关系的研究提供分子生物学依据。结果表明：从27个ISSR引物中共筛选出14个多态性明显的引物；在53份供试材料中共扩增出189条谱带，其中多态性条带189条，多态性为100%；材料间遗传相似系数范围在0.44～0.99，聚类分析结果显示，在0.62水平上53株菌株分为8组，多数菌株之间遗传相似性较低。这表明供试菌株在DNA水平上存在比较显著的遗传变异和丰富的遗传多样性；同时发现供试材料间的聚类与地域无明显相关性。
- Abstract:
- Taking 53 varieties of oyster mushroom as materials,phylogenetic analysis of oyster mushrooms from different places were carried out by ISSR marker in order to provide some molecular biology evidences for research to varieties breeding and genetic relationship of mushroom.The results showed that from 27 ISSR primers,14 primers whose polymorphisms were obviously screened out.189 bands as well as 189 polymorphic bands were obtained from all the 53 tested strains,indicating that their polymorphism was 100%.The coefficients of genetic similarity ranged from 0.44 to 0.99.The results of cluster analysis showed that all 53 strains were classified into eight groups at 0.62 levels and that the coefficients of genetic similarity for majority of strains were pretty low,indicating that there were significant genetic variation and rich genetic diversity between the tested strains at the DNA level.The result also showed that there’s no significant correlation between the tested materials’ clustering and their geographic origins.
参考文献/References:
[1] 张金霞,黄晨阳,张瑞颖,等.中国栽培白灵侧耳的RAPD和IGS分析[J].菌物学报,2004(23):514-519.
[2] 马志刚,吕作舟,郑和斌,等.ISSR标记在侧耳属菌株分类学中的初步应用[J].华中农业大学学报,2006,25(1):55-59.
[3] 张建博,桂明英,刘蓓,等.分子生物学在大型真菌遗传多样性研究中的应用[J].中国食用菌,2008,27(6):3-7.
[4] 王子迎,王书通.安徽野生香菇遗传多样性及杂种优势的ISSR分析[J].菌物学报,2006,25(2):211-216.
[5] 王子迎.安徽野生香菇ISSR和PAPD分析的比较[J].安徽教育学院学报,2005,23(6):96-105.
[6] 胡芳名.CTAB法抽提香榧种子胚乳DNA的改进[J].福建林业科技,2002,29(1):1-5.
[7] 王艺红,林俊芳,张炜阳,等.食用菌DNA提取方法研究[J].食用菌,2008(3):18-20.
[8] 钟卫鸿.基因工程技术实验指导[M].北京:北京化学工业出版社,2007(6):20-25.
[9] 任海霞,宫志远,曲玲,等.平菇ISSR-PCR 反应体系影响因素研究[J].中国食用菌,2009,28(4):35-37.
[10] 张金霞,黄晨阳,管桂萍,等.白黄侧耳(Pleurotus cornucopiae)微卫星间区(ISSR)分析[J].菌物学报,2007,26(1):115-121.
相似文献/References:
[1]肖志娟,翟梅枝,许静,等.不同核桃品种的ISSR标记分析[J].北方园艺,2013,37(04):103.
[2]李 娜,张存旭,崔晓燕,等.栓皮栎ISSR反应体系的建立[J].北方园艺,2014,38(13):93.
LI Na,ZHANG Cun-xu,CUI Xiao-yan,et al.Establishment of ISSR Reaction System in Quercus variabilis [J].Northern Horticulture,2014,38(12):93.
[3]王鸿磊,丁 强,吕 蔚,等.我国双孢菇菌株与国外引进菌株间亲缘关系的ISSR分析[J].北方园艺,2014,38(11):96.
WANG Hong-lei,DING qiang,LV Wei,et al.Genetic Relationship Based on ISSR Markers Among Agaricus bisporus Strains From China and Overseas[J].Northern Horticulture,2014,38(12):96.
[4]李 娜,张存旭,刘 伟,等.毛梾ISSR-PCR反应体系的建立[J].北方园艺,2013,37(22):114.
LI Na,ZHANG Cun-xu,LIU Wei,et al.Establishment of ISSRPCR Reaction System of Cornus walteri[J].Northern Horticulture,2013,37(12):114.
[5]陈艳露,周 莉,黄麟淇,等.抗木霉平菇菌株的筛选及其生产性能评价[J].北方园艺,2013,37(17):145.
CHEN Yan-lu,ZHOU Li,HUANG Lin-qi,et al.Screening and Evaluation of Oyster Mushroom Strains Resistant to Trichoderma Disease[J].Northern Horticulture,2013,37(12):145.
[6]陈 靓,姚方杰.平菇新品种“吉平1号”的选育[J].北方园艺,2014,38(08):147.
CHEN Liang,YAO Fang-jie.Breeding of A New Pleurotus Cultivar ‘Jiping No.1’[J].Northern Horticulture,2014,38(12):147.
[7]付月月,杨洪一.平菇病毒dsRNA的提取及脱除[J].北方园艺,2013,37(12):111.
FU Yue-yue,YANG Hong-yi.Extraction and Elimination of Virus of dsRNA from Pleurotus ostreatus[J].Northern Horticulture,2013,37(12):111.
[8]刘遂飞,张志红,孙桂琴,等.不同中药渣组合替代棉籽壳栽培平菇试验[J].北方园艺,2013,37(12):155.
LIU Sui-fei,ZHANG Zhi-hong,SUN Gui-qin,et al.Research on the Chinese Medicine Residue Instead of the Cotton Seed Hull in Planting Pleurotus ostreatus[J].Northern Horticulture,2013,37(12):155.
[9]陈仁玉,刘朝贵,方琼.有机氮对平菇原基分化期胞外酶的影响[J].北方园艺,2012,36(01):153.
CHEN Ren-yu,LIU Chao-gui,FANG Qiong.Effects of Organic Nitrogen on the Activity of Extracellular Enzymes During Primordium Formation of Pleurotus ostreatus[J].Northern Horticulture,2012,36(12):153.
[10]黄萌,陈尚钘,杨光耀,等.古樟ISSR扩增条件的优化[J].北方园艺,2013,37(07):116.
HUANG Meng,CHEN Shang-xing,YANG Guang-yao,et al.Optimization of ISSR Amplification Conditions in Ancient Camphor-trees[J].Northern Horticulture,2013,37(12):116.
备注/Memo
第一作者简介：刘志曦(1989-)，男，硕士研究生，研究方向为微生物学。E-mail：snake_dragon@126.com.
责任作者：李翠新(1972-)，女，博士，副教授，硕士生导师，研究方向为食药用真菌开发与研究。E-mail：cuixinheli@163.com.
基金项目：云南省优势特色重点学科生物学一级学科建设资助项目（50097505）；
西南地区生物多样性保育国家林业局重点实验室开放基金资助项目（KL200707）；
云南省高校特色专业建设点资助项目（50116001)；
西南林业大学科研启动基金资助项目（111046）。
收稿日期：2014-01-16