DAI Lei,YANG Zhen,JIAN Zaiyou,et al.Optimization of Subculture Medium for Tissue Culture of Epimedium brevicornu Maxim.in Danjiangkou[J].Northern Horticulture,2020,44(08):108-115.[doi:10.11937/bfyy.20192763]
丹江口淫羊藿组织培养继代培养基优化
- Title:
- Optimization of Subculture Medium for Tissue Culture of Epimedium brevicornu Maxim.in Danjiangkou
- 文献标志码:
- A
- 摘要:
- 以丹江口种植的箭叶淫羊藿为试材，采用单因素试验和响应面分析的方法，比较组织培养基中6-BA、NAA及蔗糖的不同浓度对淫羊藿生长的影响，以期为淫羊藿生产过程中苗工业化生产提供参考依据。结果表明：这3个因素对淫羊藿的植株生长影响显著；在此基础上，通过响应面法对诱导生成的丛生芽继代培养基进行了优化，可得出继代培养基中NAA浓度为0.56 mg?L-1，蔗糖浓度为31.47 g?L-1，6-BA浓度为2.03 mg?L-1淫羊藿的扩繁效果较好，生长情况评分可以达到84.88。因此这种继代培养基可提高淫羊藿繁殖力系数，可解决淫羊藿的野外增殖能力弱，且野生种苗极难存活的问题。
- Abstract:
- Taking Epimedium brevicornu Maxim.in Danjiangkou as materials,the effects of different concentrations of 6-BA,NAA and sucrose in tissue culture medium on the growth of Epimedium brevicornu Maxim.were compared by using the method of single factor experiment and response surface analysis,in order to provide reference for factory production of Eimedium brevicoru.The results showed that these three factors had significant effects on the growth of Epimedium brevicornu Maxim..On the basis,the induced shoot bud subculture medium was optimized by response surface methodology,and the propagating effect of Epimedium brevicornu Maxim.was better,when the concentration of NAA was 0.56 mg?L-1，the concentration of sucrose was 31.47 g?L-1,and the concentration of 6-BA was 2.03 mg?L-1 in the subculture medium,and the growth score could reach 84.88.Therefore,the subculture medium could improve the coefficient of fertility of Epimedium brevicornu Maxim.,which could solve the problem that the wild-type proliferative ability of Epimedium brevicornu Maxim.was weak and the wild seedlings were extremely difficult to survive.
参考文献/References:
[1]韩冰,杨峻山.淫羊藿药理作用研究概况[J].中草药,2000,31(11):873-875.[2]赵文龙,王惠珍,马毅,等.4种基原的淫羊藿适宜生境分布研究[J].中国中医药信息杂志,2019,26(2):1-5.[3]徐艳琴,李作洲,张学军,等.三种药用淫羊藿的地理分布与资源调查[J].武汉植物学研究,2008(1):91-98.[4]张华峰,杨晓华,郭玉蓉,等.药用植物淫羊藿资源可持续利用现状与展望[J].植物学报,2009,44(3):363-370.[5]魏德生,胡宝成,付小兵,等.巫山淫羊藿保护抚育种植试验初报[J].现代中药研究与实践,2010,24(5):14-16.[6]盛茂银,杨政富,林菊,等.粗毛淫羊藿幼叶愈伤组织的生产[J].种子,2005(9):22-24.[7]周海琴.巫山淫羊藿组织培养研究[D].南京:南京农业大学,2012.[8]龚娜,杨镇,李学龙,等.朝鲜淫羊藿组织培养关键技术研究[J].广东农业科学,2015,42(5):15-18.[9]杜真辉.箭叶淫羊藿栽培过程中关键技术研究[D].郑州:河南中医药大学,2017.[10]彭文书,马莉,赵苗苗,等.响应面试验优化铁皮石斛组培苗生根培养基[J].中药材,2017,40(9):2013-2017.[11]李莉,张赛,何强,等.响应面法在试验设计与优化中的应用[J].实验室研究与探索,2015,34(8):41-45.[12]孙超,林昌虎,邹剑灵,等.淫羊藿试种初报[J].中药材,2003,26(8):544-545.[13]李作洲,徐艳琴,王瑛,等.淫羊藿属药用植物的研究现状与展望[J].中草药,2005,36(2):289-295.[14]韩素菊,黎云祥,胥晓,等.箭叶淫羊藿胚性愈伤组织诱导诱导及其黄酮类化合物的总含量测定[J].中国中药杂志,2007,32(23):2551-2553.[15]李景滨,易继财,张宗申.金铁锁组培苗快繁技术研究[J].广东农业科学,2011(2):29-31.[16]王永香,米慧娟,张传力,等.Box-Behnken响应面法优化热毒宁注射液金银花和青蒿(金青)的醇沉工艺研究[J].中草药,2015,46(5):671-678.
相似文献/References:
[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]曹 虹,金荣荣.三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究[J].北方园艺,2014,38(13):106.
CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(08):106.
[4]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(08):80.
[5]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(08):84.
[6]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(08):101.
[7]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(08):89.
[8]赵丽莉,徐芳芳,荀 洋,等.桔梗花药愈伤组织诱导培养基筛选[J].北方园艺,2014,38(01):95.
ZHAO Li-li,XU Fang-fang,XUN Yang,et al.Screening of Callus Induction Medium of Platycodon grandiflorumAnther[J].Northern Horticulture,2014,38(08):95.
[9]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(08):106.
[10]陈丽文,杨利平,荣薏,等.金心也门铁工厂化育苗技术研究[J].北方园艺,2013,37(13):138.
CHEN Liwen,YANG Li-ping,RONG Yi,et al.Study on the Techniques of Industrialized Seedling Culture of Draceana arborea[J].Northern Horticulture,2013,37(08):138.
备注/Memo
第一作者简介：代磊（1982-），男，博士，讲师，现主要从事植物学及药用植物资源等研究工作。E-mail:daileinihao@126.com.责任作者：孟丽（1959-），女，本科，教授，博士生导师，现主要从事植物资源的开发和利用等研究工作。E-mail：histml@163.com.基金项目：河南省高等学校重点科研资助项目（15B180003）；河南科技学院博士科研启动基金资助项目（201010613003）；河南科技学院博士后科研启动基金资助项目（103020218003）。收稿日期：2019-09-20