[1]张丽荣,陈杭,康萍芝,等.木霉制剂在西瓜土壤中定殖能力及其对根际土壤微生物区系的影响[J].北方园艺,2018,42(21):49-53.[doi:10.11937/bfyy.20180974]
　ZHANG Lirong,CHEN Hang,KANG Pingzhi,et al.Colonization Ability of Trichoderma harzianum Preparation and Effects on Microflora in Rhizosphere Soil of Watermelon[J].Northern Horticulture,2018,42(21):49-53.[doi:10.11937/bfyy.20180974]
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木霉制剂在西瓜土壤中定殖能力及其对根际土壤微生物区系的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年21 页码: 49-53 栏目: 出版日期: 2018-11-10
Title:
Colonization Ability of Trichoderma harzianum Preparation and Effects on Microflora in Rhizosphere Soil of Watermelon
作者:
张丽荣1; 陈杭2; 康萍芝1; 杜玉宁1; 张治科1
（1.宁夏农林科学院 植物保护研究所，宁夏植物病虫害防治重点实验室，宁夏 银川 750002;2.宁夏农林科学院 农产品质量标准与技术研究所，宁夏 银川 750002）
Author(s):
ZHANG Lirong1; CHEN Hang2; KANG Pingzhi1; DU Yuning1; ZHANG Zhike1
(1.Institute of Plant Protection,Ningxia Academy of Agriculture and Forestry Sciences,Ningxia Key Laboratory of Plant Disease and Pest Control,Yinchuan,Ningxia 750002;2.Institute of Agricultural Products Quality Standards and Technology,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002)
关键词:
木霉制剂; 西瓜; 定殖能力; 根际土壤微生物
Keywords:
Trichoderma harzianum preparation; watermelon; colonization ability; microorganisms in rhizosphere soil
DOI:
10.11937/bfyy.20180974
文献标志码:
A
摘要:
以连作8年的西瓜土壤为试材，采用盆栽试验方法，利用传统平板菌落计数法研究了施用4种木霉制剂后对西瓜根际的定殖能力，及其对根际土壤微生物区系和尖孢镰刀菌的影响。结果表明：施用4种木霉制剂后改变了西瓜根际土壤微生物种群数量，其中以Ty3制剂处理效果最为突出。施入42 d时，与对照相比，西瓜根际土壤细菌和放线菌数量分别增加82.35%和295.23%；而真菌数量和尖孢镰刀菌数量减少24.29%和24.16%；定殖试验结果表明，Ty3制剂施入14 d时，木霉在西瓜根际土壤中的定殖量最大，比对照增加14 077.08%，之后基本保持稳定，施入56 d时仍比对照高出156.7 倍。表明木霉制剂施用后可显著提高西瓜根际土壤中有益微生物数量，有效抑制尖孢镰刀菌数量的增长，减轻了连作障碍的根际微生态效应。
Abstract:
The soil of watermelon with continuous cropping 8 years was used as the test material,the colonization ability of 4 kinds of Trichoderma harzianum preparation in rhizosphere of watermelon was studied by using the traditional plate colony counting method,and its influence on rhizosphere soil microbial flora and Fusarium oxysporum was investigated by pot experiment.The results showed that the number of microbial populations in rhizosphere soil of watermelon was changed after the application of 4 Trichoderma harzianum preparation,and the effect of Ty3 preparation was the most prominent.The number of bacteria and actinomycetes in rhizosphere soil increased by 82.35% and 295.23%,respectively,compared with the control,and the number of fungi and Fusarium oxysporum decreased by 24.29% and 24.16%.The results of colonization test showed that the colonization of Trichoderma harzianum preparation in the rhizosphere soil of watermelon was the largest in the 14 days,and the Ty3 was increased by 14 077.08%,then basically remained stable,and was 156.7 times higher than control when applied for 56 days.The results showed that Trichoderma harzianum preparation could significantly increase the number of beneficial microorganisms in rhizosphere soil of watermelon,effectively inhibit the growth of the number of Fusarium oxysporum and reduce the rhizosphere micro-ecological effect of continuous cropping obstacle.

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备注/Memo

第一作者简介：张丽荣（1965-），女，宁夏银川人，本科，副研究员，研究方向为植物病害生物防治及土壤微生物学。E-mail：zlrch@163.com.基金项目：宁夏自然科学基金资助项目(NZ16100)；国家公益性行业（农业）科研专项资助项目（201503110-08）。收稿日期：2018-06-07

更新日期/Last Update: 2018-11-21