[1]黄静,赵佳,陈哲,等.一株侵染草莓的落叶松葡萄座腔菌的分离鉴定及拮抗菌筛选[J].北方园艺,2018,42(03):55-60.[doi:10.11937/bfyy.20172644]
　HUANG Jing,ZHAO Jia,CHEN Zhe,et al.Isolation and Identification of Botryosphaeria laricina of Strawberry and Screening of Antagonistic Bacteria[J].Northern Horticulture,2018,42(03):55-60.[doi:10.11937/bfyy.20172644]
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一株侵染草莓的落叶松葡萄座腔菌的分离鉴定及拮抗菌筛选

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年03 页码: 55-60 栏目: 出版日期: 2018-02-15
Title:
Isolation and Identification of Botryosphaeria laricina of Strawberry and Screening of Antagonistic Bacteria
作者:
黄静; 赵佳; 陈哲; 梁宏
山西省农业科学院 生物技术研究中心，山西 太原 030031
Author(s):
HUANG Jing; ZHAO Jia; CHEN Zhe; LIANG Hong
Biotechnology Rresearch Center,Shanxi Academy of Agriculture Science,Taiyuan,Shanxi 030031
关键词:
落叶松葡萄座腔菌; 草莓; 拮抗菌; 筛选
Keywords:
Botryosphaeria laricina; strawberry; antagonistic bacteria; screening
DOI:
10.11937/bfyy.20172644
文献标志码:
A
摘要:
以患根腐病的草莓植株为试材，采用组织分离法获得了一株菌株，通过分子生物学技术对该菌株进行鉴定，并测定了其菌丝生长的最佳条件；同时，通过平板对峙法研究了解淀粉芽孢杆菌Y-48、Lh-1和CM3对此病原菌的抑制作用，以期为根腐病病原菌分离及生防菌株筛选等提供参考依据。结果表明：通过18S rDNA序列分析鉴定，菌株BL2016-6为落叶松葡萄座腔菌；菌株BL2016-6生长的适合温度为20~30 ℃，25 ℃为最适温度，菌丝由白色变为灰色，最终变成黑色，菌株致死条件为为70 ℃，处理20 min；解淀粉芽孢杆菌Y-48、Lh-1和CM3均对此病原菌有抑制作用，抑菌率分别为64.6%、62.2%和57.9%。
Abstract:
The root of strawberry infected by root rot was used as material,the strain was obtained by using the method of tissue isolation,and was identified based on the molecular biological technology,and the optimum growth conditions of mycelium were determined.At the same time,the inhibitory effect of Bacillus amyloliquefaciens Y-48,Lh-1 and CM3 on the strain was studied by plate confrontation method,in order to supply references for root rot bacteria isolation and screening antagonistic bacteria.The results showed that the strain named that was identified as Botryosphaeria laricina by 18S rDNA sequence analysis.The temperature range for strain BL2016-6 growth was 20-30 ℃,with the optimum temperature at 25 ℃.The color of mycelial changed from white to gray,eventually turned black.In addition,the lethal temperature for mycelial growth was 70 ℃ for 20 minutes.The results of antibacterial test showed that the inhibitory effects of Bacillus amyloliquefaciens Y-48,Lh-1 and CM3 on the strain BL2016-6 were 64.6%,62.2% and 57.9%,respectively.

参考文献/References:

[1]尚衍重.落叶松枯梢病菌分类地位的探讨[J].菌物学报,1987,6（4):58-59.

[2]刘秀嶶,王峰,刘雪峰.落叶松枯梢病分子检测初步研究[J].植物检疫,2009,23（2):1-4.
[3]伊藤一雄.林木图说新讲[M].东京：地球出版社株式会社，1964:264-304.
[4]项存悌.落叶松枯梢病的研究[J].东北林业大学学报,1979（1）:32-43.
[5]苑健羽.落叶松真菌病害[M].北京:科学出版社,1990.
[6]刘秀嶶.落叶松枯梢病的生物防治与分子检测初步研究[D].哈尔滨:东北林业大学,2009.
[7]常玉军,赵金娥,李晓东,等.小五台山区落叶松枯梢病特性与防治[J].河北林业科技,2011（2):82.
[8]付晓霞,王志明,王选遥,等.落叶松落叶病和枯梢病合并防治研究[J].吉林林业科技,2016,45（2):30-34.
[9]DENMAN S,CROUS P W,GROENEWALD J Z,et al.Circumscription of Botryosphaeria species associated with Proteaceae based on morphology and DNA sequence data[J].Mycologia,2003,95（2):294.
[10]姜晓龙,金磊磊,陈辉辉,等.1株侵染蓖麻的葡萄座腔菌菌株鉴定[J].江苏农业科学,2015,43（2):319-322.
[11]周家华,严松俊,王承芳,等.一株分离自桑疫病植株的真菌鉴定及对桑树的致病性分析[J].蚕业科学,2013,39（6):1066-1070.
[12]赵佳,黄静,陈哲,等.西瓜枯萎病拮抗菌Lh-1的鉴定及生物防治效果研究[J].生物技术通报,2017,33（4):130-136.
[13]黄静,赵佳,陈哲,等.抗草莓灰霉病的芽孢杆菌CM3的分离与筛选[J].北方园艺,2016（11):113-116.
[14]王凤各,叶建仁.JK-JS8菌株对松树枯梢病菌的拮抗作用及机理[J].辽东学院学报（自然科学版),2016,23（2):117-121.
[15]邓勋,宋瑞清,宋小双,等.高效木霉菌株对樟子松枯梢病的抑菌机理[J].中南林业科技大学学报,2012,32（11):21-27.

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备注/Memo

第一作者简介：黄静（1965-），女，本科，副研究员，现主要从事拮抗菌抑菌物质开发等研究工作。E-mail:362948115@qq.com.

责任作者：梁宏（1964-），男，本科，副研究员，研究方向为微生物学。E-mail:lh1964@126.com.
基金项目：山西省农业科学院科技攻关资助项目（YGG17067）；山西省科技自主创新能力提升资助项目（2015zzcx-22）；山西省青年科技研究基金资助项目（201601D202062）。
收稿日期：2017-10-18

更新日期/Last Update: 2018-02-09