LU Zhiwei,HOU Xiaowan,GAO Jianming,et al.Effect of Colchicine Treatment on Asparagus ‘Dc Paoli F1’ for Tetraploid Induction[J].Northern Horticulture,2017,41(02):23-30.[doi:10.11937/bfyy.201702005]
秋水仙素处理对芦笋“达宝利”四倍体诱导效果的影响
- Title:
- Effect of Colchicine Treatment on Asparagus ‘Dc Paoli F1’ for Tetraploid Induction
- Keywords:
- asparagus; tetraploid; colchicines; induction; breeding
- 文献标志码:
- A
- 摘要:
- 以芦笋“达宝利”露白种子为试材，秋水仙素（添加2％ DMSO）处理浓度和处理时间作为诱导因子，设计多个梯度的正交实验，研究秋水仙素对芦笋四倍体诱导率的影响，以期获得最佳的芦笋多倍体诱导条件。结果表明：最佳的秋水仙素处理组合为0.15％的秋水仙素（添加2％ DMSO）处理16 h，诱导率高达28.33％；经鉴定发现二倍体芦笋的染色体数目为20条，而四倍体染色体数目为40条；与二倍体相比，四倍体芦笋植株粗壮、叶片长度和直径增加，气孔细胞密度减少，气孔细胞体积增大，叶绿体数目和叶绿素含量增加。
- Abstract:
- The sprouting seeds of the ‘Dc Paoli F1’asparagus varieties were selected as the experimental materials.With the treating concentrations and time of colchicine (2% DMSO added) as inducing factors，multiple gradients of orthogonal experiments were designed to study the effect of colchicine on asparagus tetraploid induction rate and identify the optimum treating combinations of colchicine.The results showed that the combination of 0.15% colchicine (2% DMSO added) with 16 hours was the best treatment combinations with an induction rate of up to 28.33%.The number of diploid and tetraploid chromosome in asparagus were 20 and 40 respectively.Compared with diploid asparagus，tetraploid asparagus had thicker individual，longer leaf，increased diameter，decreased stomatal cells density，larger stomatal cells volume，more chloroplast and chlorophyll concentration.
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备注/Memo
第一作者简介：鹿志伟（1990-），男，硕士研究生，研究方向为作物育种。E-mail：luzhiweihn@126.com.责任作者：易克贤（1964-），男，博士，研究员，现主要从事作物遗传育种等研究工作。E-mail：yikexian@126.com.基金项目：湛江市热带作物遗传改良重点实验室建设资助项目（2015A06005）；海南省应用技术研发与示范推广专项资助项目（ZDXM2015047）。