FENG Wenhua,DAI Hongjun.Study on Tissue Culture and Rapid Propagation System of ‘Cabernet Sauvignon’ Grape[J].Northern Horticulture,2016,40(13):104-106.[doi:10.11937/bfyy.201613027]
“赤霞珠”葡萄组培快繁体系研究
- Title:
- Study on Tissue Culture and Rapid Propagation System of ‘Cabernet Sauvignon’ Grape
- Keywords:
- ‘Cabernet Sauvignon’ grape; tissue culture; explant
- 文献标志码:
- A
- 摘要:
- 以“赤霞珠”葡萄为试材，对组培快繁中的取材部位、消毒方法、不同激素用量、是否带叶继代培养等关键技术措施进行了研究探讨。结果表明：以半木质化，0.1%升汞溶液消毒8 min的茎段接种，成活率高，污染率低；并且继代培养和生根培养最好的培养基为1/2MS+IBA 0.2 mg?L-1+蔗糖20 g?L-1+琼脂7 g?L-1。
- Abstract:
- Taking‘Cabernet Sauvignon’ grape as test material,on the tissue culture and rapid propagation of sampling location,disinfection methods,different hormone dosage,leaves subculture of key technical measures were discussed.The results showed that the semi lignified,0.1% HgCl2 disinfection solution for 8 min of stems were inoculated,the survival rate was high,low pollution rate；and subculture and rooting culture medium for the best was 1/2MS+IBA 0.2 mg?L-1 sucrose 20 g?L-1+agar 7 g?L-1.
参考文献/References:
[1]刘世松.中国葡萄酒产业面临的危机与对策[J].酿酒,2011,38(5):80-83. [2]刘世松.进口葡萄酒对我国葡萄酒产业的影响及对策[J].中国市场,2011(45):157-163. [3]张发维,罗会贤,李晓松,等.优质果苗的繁殖技术总结[J].中国园艺文摘,2011,27(10):152-155. [4]GARCA-GONZLES R,QUIROZ K,CARRASCO B,et al.Plant tissue culture:current status,opportunities and challenges[J].Cienc Inv Agr,2010,37(3):5-30. [5]DIAB A A,KHALIL S M,ISMAIL R M.Regeneration and micropropagation of grapevine(Vitis vinifera L.)through shoot tips and axillary buds[J].IJABR,2011,2(4):484-491. [6]STAMP J A,COLBY S M,MEREDITH C P.Direct shoot organogenesis and plant regeneration from leaves of grape(Vitis spp.)[J].Plant Cell Tiss Org Cult,1990,22:127-133. [7]TORREGROSA L,BOUQUET A.Adventitious bud formation and shoot development from in vitro leaves of Vitis×Muscadinia hybrids[J].Plant Cell Tiss Org Cult,1996,45:245-252. [8]HELOIR M C,FOURNIOUX J C,OZIOL L,et al.An improved procedure for the propagation in vitro of grapevine(Vitis vinifera cv.Pinot noir)using axillary-bud microcuttings[J].Plant Cell Tiss Org Cult,1997,49:223-225. [9]CHUONG P V,BEVERSDORF W D.High frequency embryogenesis through isolated microspore culture in Brassica napus L.and B.carinata braun[J].Plant Sci,1985,39(3):219-226. [10]李永辉,白远国,邓红云,等.不同浓度6-BA对“红标无核”葡萄茎段培养的影响[J].安徽农业科学,2007,35(15):4452-4453. [11]周恒,万召娣,罗静.紫秋刺葡萄离体繁殖研究初报[J].贵州农业科学,2010,38(9):22-25. [12]GRAY D J,BENTON C M.Micropropagation and plant establishment of muscadine grape[J].Proc Fla State Hort Soc,1990,103:300-302. [13]GRAY D J,BENTON C M.In vitro micropropagation and plant establishment of muscadine grape cultivars(Vitis rotundifolia)[J].Plant Cell Tiss Org Cult,1991,27:7-14.
相似文献/References:
[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]曹 虹,金荣荣.三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究[J].北方园艺,2014,38(13):106.
CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(13):106.
[4]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(13):80.
[5]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(13):84.
[6]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(13):101.
[7]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(13):89.
[8]赵丽莉,徐芳芳,荀 洋,等.桔梗花药愈伤组织诱导培养基筛选[J].北方园艺,2014,38(01):95.
ZHAO Li-li,XU Fang-fang,XUN Yang,et al.Screening of Callus Induction Medium of Platycodon grandiflorumAnther[J].Northern Horticulture,2014,38(13):95.
[9]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(13):106.
[10]陈丽文,杨利平,荣薏,等.金心也门铁工厂化育苗技术研究[J].北方园艺,2013,37(13):138.
CHEN Liwen,YANG Li-ping,RONG Yi,et al.Study on the Techniques of Industrialized Seedling Culture of Draceana arborea[J].Northern Horticulture,2013,37(13):138.
备注/Memo
第一作者简介：冯文华（1991-），女，硕士研究生，研究方向为葡萄栽培学。E-mail：415369173@qq.com.责任作者：代红军（1967-），女，博士，教授，现主要从事葡萄栽培与生理等研究工作。E-mail：dai-hj@nxu.edu.cn.基金项目：国家自然科学基金资助项目（31260456）。