ZHANG Yanqiu,QU Lianwei,SU Junwei,et al.Study on Cryopreservation of Shoot Tips From in vitro Plants of Chrysanthemum by Vitrification Method[J].Northern Horticulture,2016,40(05):128-132.[doi:10.11937/bfyy.201605034]
菊花茎尖玻璃化法超低温保存技术研究
- Title:
- Study on Cryopreservation of Shoot Tips From in vitro Plants of Chrysanthemum by Vitrification Method
- Keywords:
- Chrysanthemum; tissue culture; shoot tips; cryopreservation
- 文献标志码:
- A
- 摘要:
- 以菊花品种“神马”为试材，采用单因素逐级优化试验设计方法,研究了蔗糖浓度、预培养时间、装载时间、玻璃化处理时间、茎尖大小以及冻存时间对茎尖存活率的影响。结果表明：菊花茎尖玻璃化超低温长期保存最佳处理组合为预培养蔗糖浓度0.25 mol/L，预培养时间3 d，装载处理温度25℃、40 min，玻璃化处理温度0℃、60 min，菊花茎尖大小1.5～2.0 mm。采用此体系保存菊花茎尖180 d 后，经卸载及恢复培养，存活率可达71.33%。
- Abstract:
- Chrysanthemum ‘Jinba’ was used as test material，and the experiment design of single factor optimization was used to research the effect of sucrose concentration in pre-culture medium，time of pre-culture，loading，exposure of PVS-2，size of shoot tips and the time of frozen on cryopreservation.The results showed that the optimal treatment combination realizing long-period preservation of the shoot tip of Chrysanthemum by vitrification method including：0.25 mol/L of sucrose in pre-culture medium，pre-culturing 3 days，loading treatment at 25℃ for 40 minutes，vitrification process at 0℃ for 60 minutes and 1.5-2.0 mm of shoot tips.The survival rate of shoot tips preservated for 180 days using this system could reach 71.33% after unloading and recovery training.
参考文献/References:
[1]蔡斌华,张计育,渠慎春,等.通过玻璃化超低温处理脱除草莓轻型黄边病毒(SMYEV)研究[J].果树学报,2008,25(6):872-876. [2]曾继吾,牛王翠,黄永红,等.利用超低温保存方法脱除香蕉束顶病毒的研究[J].植物遗传资源学报,2009,10(3),457-460. [3]王仁睿,李明福.葡萄、马铃薯种质超低温脱毒应用初步研究[C].中国植物病理学会2010年学术年会论文集,2010. [4]冯超红.苹果茎尖超低温保存技术及脱毒效率的研究[D].杨凌:西北农林科技大学，2014. [5]陈辉,陈晓玲,陈龙清,等.切花百合离体茎尖玻璃化法超低温保存研究[J].植物遗传资源学报,2007,8(2):170-173. [6]王秋竹,林丽华,董文轩.月季茎尖玻璃化法超低温保存技术研究[J].沈阳农业大学学报,2009,40(2):156-159. [7]王君晖,黄纯农.玻璃化法-园艺作物茎尖和分生组织超低温保存的新途径-文献综述[J].园艺学报,1994,21(3):277-282. [8]TSKAGI H,TINH N,ISLAM O M.Cryopreservation of in vitro-grown shoot tips of vitrification procedure[J].Plant Cell Rep,1997（16）:594-599. [9]刘祖祺,张石城.植物抗性生理学[M].北京:中国农业出版社,1994. [10]MATSUMOTO T,TAKAHASHI C,SAKAI A,et al.Crypreservation of in vitro-grown apical meristems of hybrid statice by three different procedures[J].Scientia Horticture,1998,76:105-114. [11]王子成,邓秀新.玻璃化法超低温保存柑桔茎尖及植株再生[J].园艺学报,2001,28(4)：301-309. [12]王贞.扶芳藤茎尖玻璃化超低温保存及其效果评价[D].北京:北京林业大学,2007. [13]刘艳霞.菊花茎尖超低温保存及遗传稳定性评价[D].武汉:华中农业大学,2008. [14]周小梅,王国英,敖光明,等.玉米组培材料的玻璃化法超低温保存及冻后植株再生[J].农业生物技术学报,2001,9(4):355-358. [15]薛建平.地黄试管块根诱导及茎尖玻璃化法超低温保存[D].武汉:华中农业大学,2004. [16]吕长平,鸾爱萍,陈海霞,等.玻璃化法超低温保存非洲菊茎尖及植株再生[J].中国观赏园艺研究进展，2012(4):307-310. [17]王彪.马铃薯茎尖超低温保存及超低温疗法脱毒技术体系的建立[D].杨凌:西北农林科技大学,2011. [18]曹庆,易干军,陈金印,等.柑橘裂皮病类病毒超低温脱毒技术的初步研究[J].中国南方果树,2013,42(5):63-67. [19]殷晓辉,舒理慧.植物种质资源的超低温保存研究进展[J].热带亚热带植物学报,1996,4(3):75-82.
相似文献/References:
[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]马丽.几种试剂组合对菊花切花保鲜效果的影响[J].北方园艺,2013,37(04):135.
[4]曹 虹,金荣荣.三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究[J].北方园艺,2014,38(13):106.
CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(05):106.
[5]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(05):80.
[6]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(05):84.
[7]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(05):101.
[8]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(05):89.
[9]赵丽莉,徐芳芳,荀 洋,等.桔梗花药愈伤组织诱导培养基筛选[J].北方园艺,2014,38(01):95.
ZHAO Li-li,XU Fang-fang,XUN Yang,et al.Screening of Callus Induction Medium of Platycodon grandiflorumAnther[J].Northern Horticulture,2014,38(05):95.
[10]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(05):106.
[11]王自布,莫国秀,罗会兰,等.菊花不同外植体组培快繁及其再生体系的研究[J].北方园艺,2015,39(18):106.[doi:10.11937/bfyy.201518028]
WANG Zibu,MO Guoxiu,LUO Huilan,et al.Research of Tissue Culture and Regenerated of the Different Explants of Chrysanthemum indicum L.[J].Northern Horticulture,2015,39(05):106.[doi:10.11937/bfyy.201518028]
备注/Memo
第一作者简介：张艳秋（1981-），女，辽宁阜新人，硕士，助理研究员，日本岐阜大学访问学者，现主要从事花卉育种与栽培等研究工作。E-mail：zyq810711@163.com.责任作者：屈连伟（1977-），男，辽宁辽阳人，博士，副研究员，荷兰瓦赫宁根大学访问学者，现主要从事观赏园艺植物新品种选育及高效栽培技术示范与推广等工作。E-mail:568219189@qq.com.