YUAN Liuxiang,LI Houhua,FU Linjiang,et al.Tissue Culture Techniques of Neillia thyrsiflora[J].Northern Horticulture,2015,39(24):87-89.[doi:10.11937/bfyy.201524025]
绣线梅组织培养技术
- Title:
- Tissue Culture Techniques of Neillia thyrsiflora
- Keywords:
- Neillia thyrsiflora; tissue culture; stem segment
- 文献标志码:
- A
- 摘要:
- 以绣线梅（Neillia thyrsiflora）的茎尖或带腋芽的茎段为外植体，采用组织培养技术，研究了绣线梅快速繁殖体系。结果表明：芽诱导最适培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L，诱导率为90%；MS+6-BA 2.0 mg/L+NAA 0.2 mg/L为最佳增殖培养基，增殖系数达7.5；1/2MS+IBA 0.2 mg/L为最佳生根培养基，生根率66.7%。
- Abstract:
- Taking stem tips and internodes with axillary buds of Neillia thyrsiflora as the explants，the rapid propagation system in vitro was studied by tissue culture techniques.The results showed that MS+6-BA 1.0 mg/L+NAA 0.2 mg/L was the suitable medium for explants induction of cluster buds,induction rate reached 90%；MS+6-BA 2.0 mg/L+NAA 0.2 mg/L was the optimizing medium for multi-propagation，and its average proliferation coefficient reached 7.5；the optimal rooting medium of shoots was 1/2MS+IBA 0.2 mg/L，the rooting rate was 66.7%.
参考文献/References:
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备注/Memo
第一作者简介：袁柳祥(1991-)，男，硕士研究生,研究方向为园林植物分子生物学。E-mail：961004274@qq.com.责任作者：李厚华（1973-），男，博士，副教授,现主要从事植物基因工程和类黄酮次生代谢等研究工作。E-mail：lihouhua73@163.com.基金项目：国家林业公益性行业科研专项资助项目（201204308）；西北农林科技大学基本科研业务费资助项目（QN2013079）。