MU Hongmei,DU Xiuju,CAO Xing,et al.Study on Tissue Culture and Rapid Propagation of Gynura formosana Kitam[J].Northern Horticulture,2015,39(21):96-98.[doi:10.11937/bfyy.201521025]
白凤菜组培快繁技术研究
- Title:
- Study on Tissue Culture and Rapid Propagation of Gynura formosana Kitam
- Keywords:
- Gynura formosana Kitam; tissue culture; stem; propagation
- 文献标志码:
- A
- 摘要:
- 以白凤菜为试材，采用组织培养方法，研究了氯化汞浸泡时间及不同激素配比对白凤菜茎段灭菌及诱导的影响。结果表明：外植体最适宜的灭菌条件为0.1%的氯化汞溶液浸泡11 min，此时污染率最低为41%，外植体成活率为68%，为较适宜的灭菌时间。MS+6-BA 3.0 mg/L+NAA 0.02 mg/L为比较适宜的白凤菜茎段诱导培养基，诱导率达98%，每块外植体分化的不定芽数目达到8.0个；白凤菜的生根培养基为1/2 MS+NAA 0.20 mg/L。
- Abstract:
- The stem of Gynura formosana Kitam was used as explants，tissue culture method was used，and the effect of mercuric chloride soaking time and different hormone combinations on the induction of stem segment of Gynura formosana Kitam were studied.The results showed that，when the explants’ sterilization time was 11 minutes with 0.1% mercuric chloride solution，the contamination rate of explants was minimum (41%)，the survival rate was 68%.The stem suitable induction culture medium was MS+6-BA 3.0 mg/L+NAA 0.02 mg/L，at this time the induction rate reached 98%，the number of buds per explants reached 8.0.The suitable induction culture of rooting medium was 1/2 MS+NAA 0.20 mg/L.
参考文献/References:
[1]汪泓江,梁呈元,卓敏,等.Gynura属3个野生蔬菜营养成分的比较及评价[J].中国野生植物资源,2004(5):48-49. [2]胡勇,李维林,林厚文,等.白背三七地上部分降血糖作用研究[J].西南林学院学报,2007,27(1):55-58. [3]汪洪江,李维林,任冰如,等.白凤菜扦插繁殖技术研究[J].中国野生植物资源,2010,29(3):62-65. [4]穆红梅.中国石蒜（Lycoris chinensis）体内加兰他敏等生物碱积累影响因素的研究[D].南京:南京农业大学,2009.
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备注/Memo
第一作者简介：穆红梅（1974-），女，博士，高级实验师，现主要从事园林和资源植物的生物技术等研究工作。E-mail：muhongmei74@163.com.基金项目：国家自然科学基金资助项目(31301802)；山东省教育厅自然科学基金资助项目(ZR2011CL010)；国家级大学生科技创新资助项目（201410447016）。