WU Yue-liang,ZHAO Chuan-tong,YI Wen-zhi,et al.Effect of Pollen Viability of Xanthoceras sorbifolia Under Different Culture Media and Storage Conditions[J].Northern Horticulture,2015,39(05):64-67.[doi:10.11937/bfyy.201505022]
不同培养基及贮藏条件对文冠果花粉生活力的影响
- Title:
- Effect of Pollen Viability of Xanthoceras sorbifolia Under Different Culture Media and Storage Conditions
- 文献标志码:
- A
- 摘要:
- 以文冠果的花粉为试材,研究不同培养基及贮藏条件对文冠果花粉生活力的影响。结果表明:培养基中硼酸对花粉萌发的影响最大,蔗糖次之,琼脂最小;适合文冠果花粉萌发的培养基中蔗糖浓度为10%~15%,硼酸浓度为0.01%~0.05%,琼脂浓度为0.5%~0.6%;贮藏条件对文冠果花粉生活力有很大影响,-20℃冷冻条件下有利于保持花粉的生活力,4℃低温贮藏次之,25℃条件不利于花粉的长期贮藏。该研究可为文冠果生产上的花粉贮藏和人工授粉提供参考依据。
- Abstract:
- Taking pollen of Xanthoceras sorbifolia as material,the pollen viability of Xanthoceras sorbifolia under the different culture media and the storage conditions was studied.The results showed that,the effect of boric acid on pollen germination was the greatest in the culture medium,followed by sucrose and agar.The suitable sucrose concentration to pollen viability was 10%~15% in the culture medium,boric acid concentration was 0.01%~0.05%,and agar concentration was 0.5%~0.6%.Storage temperature had great effect on pollen viability.-20℃ was favorable for keeping pollen viability,and 4℃ took the second place,and 25℃ was not conducive to the pollen storage.So reference basis for pollen-storage and artificial pollination of Xanthoceras sorbifolia in the production was offered.
参考文献/References:
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备注/Memo
第一作者简介:吴月亮(1972-),男,辽宁铁岭人,博士,副教授,现主要从事园林植物与林木育种等研究工作。E-mail:wuyueliang72@163.com.责任作者:刘明国(1964-),男,辽宁朝阳人,博士,教授,现主要从事森林培育及经济林种质资源等研究工作。基金项目:沈阳农业大学“林业种质创新与培育”学术创新团队资助项目(2013-4-B-2)