SONG Qun-yan,WANG Li-yan,JIAO Hong-shuang,et al.Study on Tissue Culture and Plant Regeneration in Castorplant Regeneration of Xanthoceras sorbifolia Bunge.[J].Northern Horticulture,2013,37(07):121-124.
文冠果组织培养和植株再生研究
- Title:
- Study on Tissue Culture and Plant Regeneration in Castorplant Regeneration of Xanthoceras sorbifolia Bunge.
- 文章编号:
- 1001-0009(2013)07-0121-04
- Keywords:
- Xanthoceras sorbifolia; callus; plant regeneration
- 分类号:
- S 668.903.6
- 文献标志码:
- A
- 摘要:
- 以文冠果实生苗幼嫩叶片为外植体，研究了植物生长调节剂种类及配比对文冠果组织培养及植株再生的影响，经愈伤诱导、增殖、分化、伸长和生根培养,获得了再生植株，建立了文冠果体细胞胚发生及再生植株体系。结果表明：最适合文冠果胚性愈伤诱导的培养基为DKW+6-BA 0.5 mg/L+NAA 0.5 mg/L+2,4-D 1.0 mg/L；最适分化及增殖的培养基为DKW+6-BA 0.5 mg/L+NAA 0.5 mg/L+KT 1.0 mg/L；伸长培养基为DKW+6-BA 0.2 mg/L+IBA 0.5 mg/L；生根培养基为1/2MS+IBA 0.5 mg/L，生根率为81％，移栽成活率可达到90％以上。该再生体系的建立为文冠果快速繁殖和遗传转化的研究奠定了基础。
- Abstract:
- Taking the tender leaves of Xanthoceras sorbifolia as explants，the effect of species and concentrations of plant growth regulator on tissue culture and plant regeneration of Xanthoceras sorbifolia were studied，after the following four steps：adventitious shoot induction，induction and proliferation and differentiation，root induction，and root growth，an effective regeneration system was established for Xanthoceras sorbifolia.The results showed that the optimal medium for callus induction was DKW+6-BA 0.5 mg/L+NAA 0.5 mg/L+2,4-D 1.0 mg/L；for callus differentiation and proliferation the optimal medium was DKW+6-BA 0.5 mg/L+NAA 0.5 mg/L+KT 1.0 mg/L；for elongating DKW+6-BA 0.2 mg/L+IBA 0.5 mg/L，and for rooting，1/2MS +IBA 0.5 mg/L；the ratio of rooting was 81%，and that of survival was more than 90% when small intact plants were transplanted.The establishment of this regeneration system had laid a foundation for rapid propagation and genetic transformation of Xanthoceras sorbifolia.
参考文献/References:
[1]高述民,马凯,杜希华,等.文冠果(Xanthoceras sorbifolia)研究进展[J].植物学通报,2002,19(3):296-301. [2]崔红.文冠果育苗技术[J].山西林业,2004(4):26-27. [3]王中山,王玉欣.文冠果[J].特种经济动植物,2002(11):29. [4]张桂琴,徐祥龄,赵志学.文冠果嫩茎组织诱导植株移栽初获成功[J].林业科技通迅讯,1980(7):4-5. [5]柳金凤,吴建华,闵丽霞.文冠果组培快繁技术研究[J].江苏农业科学,2010(2):52-54. [6]盛德策.文冠果果实发育及体细胞胚培养的研究[D].北京:北京林业大学,2008. [7]臧国忠,陈尚武,张文,等.文冠果子叶同步胚的高效诱导及植株再生[J].西北林学院学报,2008,23(5):91-94. [8]王永明,赵静茹,陈颖.文冠果的组织培养[J].植物生理学通讯,1986(1):132-145. [9]王玉珍,李霞,张弛.文冠果组培快速繁殖方法[P].中国专利.101032226,2007:9-12. [10]黄永伟,贾明仁,王洪波,等.影响文冠果组织培养苗离体生根的因素[J].北方果树,2010(4):7-9.
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备注/Memo
第一作者简介：宋群雁（1983-），女，在读硕士，研究方向为生物技术。