LI Lin-ling,CHENG Hua,WU Cong-hua,et al.Simultaneous Determination of 4 Endogenous Hormones in Chinese Chestnut Floral Bud by Solid Phase Extraction-High Performance Liquid Chromatography[J].Northern Horticulture,2014,38(01):22-25.
SPE-HPLC法同时分离测定板栗花芽中四种内源激素的研究
- Title:
- Simultaneous Determination of 4 Endogenous Hormones in Chinese Chestnut Floral Bud by Solid Phase Extraction-High Performance Liquid Chromatography
- 文章编号:
- 1001-0009(2014)01-0022-04
- Keywords:
- solid phase extraction; high performance liquid chromatography; Chinese chestnut; floral bud; endogenous hormones
- 分类号:
- S 664.2
- 文献标志码:
- A
- 摘要:
- 以板栗花芽为试材，研究了建立其花芽中吲哚乙酸（IAA）、脱落酸（ABA）、玉米素核苷（ZR）、赤霉素（GA3）4种内源激素含量的定性、定量分析方法。结果表明：采用80%的预冷甲醇水溶液浸提板栗枝芽中内源激素IAA、ABA、GA3、ZR，提取效果较好；采用SPE-C18小柱固相萃取技术进行纯化，采用Symmetry 300TM C18（5 μm，4.6 mm×250 mm），以甲醇:0.075%冰醋酸（90∶5，V∶V）为流动相，在流速1.0 mL/min、柱温30℃，检测波长219 nm的条件下，进行梯度洗脱，分离效果理想，加标回收率达到89.92%~105.30%；该方法准确、灵敏、重现性好。
- Abstract:
- With Chinese chestnut floral bud as materials,an analytical method based on solid phase extraction-high performance liquid chromatography (SPE-HPLC) was established for the determination of endogenous hormones IAA,ABA,GA3 and ZR in chestnut bud.The results showed that,80% precooled methanol aqueous solution was used to extract the buds endogenous hormones IAA,ABA,GA3 and ZR from chestnut,and the result of extraction was better.Separation was performed on a Symmetry 300TM C18 column (5 μm,4.6 mm×250 mm),using a mixture of methano and 0.075% glacial acetic acid solution (90∶5,volume ratio) as mobile phase at a flow rate of 1.0 mL/min.The ultraviolet detection was set at 219 nm,column temperature at 30°C,gradient elution,the separating effect was good,and the recovery ranged from 89.92% to 105.30%.The method was proved to be accurate,sensitive and reproducible.
参考文献/References:
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备注/Memo
第一作者简介：李琳玲（1981-），女，湖北十堰人，博士，讲师，研究方向为微生物防治。E-mail：lilinling1437@126.com.