YAO Xiaotong,ZHANG Runzhi,XU Ning,et al.Analysis of Glucosinolate Content in Different Brassica Vegetables[J].Northern Horticulture,2018,42(21):30-36.[doi:10.11937/bfyy.20180890]
不同品种芸薹属蔬菜中硫代葡萄糖苷含量的比较分析
- Title:
- Analysis of Glucosinolate Content in Different Brassica Vegetables
- Keywords:
- glucosinolate; Brassica vegetable; HPLC
- 文献标志码:
- A
- 摘要:
- 以4种芸薹属蔬菜为试材，采用高效液相色谱法（HPLC）对芸薹属蔬菜中硫苷的含量及种类进行测定，以期为芸薹属蔬菜的合理轮作种植模式提供参考依据。结果表明：在“二道眉”芥菜的根、叶和雪里红中分别检测到了15、16、13种硫苷，均以2-丙烯基硫苷为主，分别占其总量的89.23%、91.71%和75.23%；而“小花英”芥菜根、叶和油菜中分别检测到了13、12、14种硫苷，主要以3-丁烯基为主，分别占其总量的30.25%、38.11%和91.80%。在4种芸薹属蔬菜中，“二道眉”芥菜根中硫苷总量最高，而“小花英”芥菜根中含量最低。不同种类的芸薹属蔬菜以及相同品种的不同基因型和同一品种的不同器官中硫苷含量和种类均有差异。
- Abstract:
- Four Brassica vegetables were used as test materials,the glucosinolates(GS) composition and contents were evaluated by HPLC,in order to provide reference for the rational rotation planting mode of Brassica vegetables.The results showed that 15,16,13 individual glucosinolates were found in the root and leaves of Brassica napiformis var. Erdaomei and Brassica juncea var. multiceps Xuelihong respectively.In addition,the main composition was 2-propenyl glucosinolate,which occupied 89.23%，91.71% and 75.23% respectively of the total glucosinolates in root and leaves of these Brassica vegetables.However,root and leaves of Brassica napiformis var.Xiaohuaying and Brassica campestris L.Woguan-2 contained 13，12,14 individual glucosinolates.3-butenyl-GS was the dominant glucosinolate,representing about 30.25%，38.11% and 91.80% respectively of the total.In these 4 different Brassica vegetables,the root of Brassica napiformis var. Erdaomei had the highest total glucosinolate content,while the lowest concentration was found in root of Brassica napiformis var.Xiaohuaying.Different Brassica vegetables had different glucosinolate content and composition,different genotypes of the same species and different organs of Brassica vegetable.
相似文献/References:
[1]王丽红,吴莉莉,武冬梅,等.HPLC内标法测定猫耳朵菜的硫苷含量[J].北方园艺,2012,36(12):128.
WANGLi-hong,WULi-li,WUDong-mei,et al.DeterminationGlucosinolatesContentinDrabanemorosaL.var.leiocarpaLindl.byHPLC[J].Northern Horticulture,2012,36(21):128.
[2]袁 惠 燕,侯 喜 林.芸薹属蔬菜花药 — — —花粉培养研究进展[J].北方园艺,2002,26(02):0.[doi:10.11937/bfyy.200202042]
[J].Northern Horticulture,2002,26(21):0.[doi:10.11937/bfyy.200202042]
[3]王小霞樊新民崔辉梅.芸薹属蔬菜花药培养研究进展[J].北方园艺,2007,31(01):0.[doi:10.11937/bfyy.200701014]
[J].Northern Horticulture,2007,31(21):0.[doi:10.11937/bfyy.200701014]
[4]赵学志,何洪巨,刘庞源,等.芜菁不同生长期硫代葡萄糖苷的变化[J].北方园艺,2018,42(05):27.[doi:10.11937/bfyy.20172978]
ZHAO Xuezhi,HE Hongju,LIU Pangyuan,et al.Glucosinolates Variation in Different Rowing Stages of Turnip[J].Northern Horticulture,2018,42(21):27.[doi:10.11937/bfyy.20172978]
备注/Memo
第一作者简介：姚小桐（1993-），女，硕士研究生，研究方向为植物营养学。E-mail：2622991609@qq.com.责任作者：李淑敏（1971-），女，博士，教授，博士生导师，研究方向为作物营养与施肥。E-mail:lishumin113@126.com.基金项目：公益性行业（农业）科研专项资助项目（201503109）；黑龙江省自然科学基金资助项目（C2015016）。收稿日期：2018-05-15