[1]步佳佳,胡玉林,魏长斌,等.利用HPLC法对香蕉根中四种内源激素测定方法的优化[J].北方园艺,2012,36(08):30-33.
　BU Jia-jia,HU Yu-lin,WEI Chang-bin,et al.The Optimum of HPLC Conditions of Four Kinds of Endogenous Hormones in the Roots of Banana[J].Northern Horticulture,2012,36(08):30-33.

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利用HPLC法对香蕉根中四种内源激素测定方法的优化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷期数: 2012年08期页码: 30-33 栏目: 出版日期: 2012-08-16

Title:: The Optimum of HPLC Conditions of Four Kinds of Endogenous Hormones in the Roots of Banana

文章编号:: 1001-0009(2012)08-0030-04

作者:: 步佳佳¹; 2; 胡玉林²; 魏长斌²; 谢江辉²; 何新华¹; 1.广西大学农学院，广西南宁 530004；
2.中国热带农业科学院南亚热带作物研究所，广东湛江 524091

Author(s):: BU Jia-jia¹; 2; HU Yu-lin²; WEI Chang-bin²; XIE Jiang-hui²; HE Xin-hua¹; 1.College of Agriculture，Guangxi University,Nanning，Guangxi 530004；
2.South Subtropical Crops Research Institute，Chinese Academy of Tropical Agricultural Science，Zhanjiang，Guangdong 524091

关键词:: 高效液相色谱; 内源激素; 香蕉; 根

Keywords:: HPLC; endogenous hormones; banana; root

分类号:: Q 946.885

文献标志码:: A

摘要:: 研究了高效液相色谱法测定香蕉根中4种内源激素：赤霉素（GA3）、3-吲哚乙酸（IAA）、脱落酸（ABA）和玉米素（ZT）的最佳条件。采用Agilent ZORBAX SB-C18柱（250 mm×4.6 mm，5 μm）进行分离测定。结果表明：GA₃、IAA、ABA测定的最佳条件是甲醇∶乙腈∶磷酸缓冲液（pH 3.5）为15∶20∶65为流动相，检测波长为210 nm。ZT测定最佳条件为：甲醇∶乙腈∶磷酸缓冲液（pH 3.5）为15∶15∶70为流动相，波长265 nm。4种激素的进样量为20 μL，流速为1 mL/min，柱温为35℃。选用外标法进行了定量测定。试验结果表明其回收率高，是一种快速有效的测定方法。

Abstract:: The detection methods of four endogenous hormones [gibberellin acid(GA₃)，indole-3-acetic acid(IAA)，abscisic acid(ABA)，zeatin(ZT)]in roots of bananas by HPLC with UV detector were established.The separation was performed on an Agilent ZORBAX SB-C₁₈ column (250 mm×4.6 mm，5 μm).The optimum chromatographic conditions were as follows：methanol∶acetonitrile∶phosphate buffer solution(pH 3.5)=15∶20∶65 as the mobile phase at 1 mL/min with an injection of 20 μL for GA₃，IAA，ABA at 210 nm；methanol∶acetonitrile∶phosphate buffer solution(pH 3.5)=15∶15∶70 as the mobile phase at 1 mL/min with an injection of 20 μL of ZT at 265 nm.A column temperature of 35℃ was optimum for the separation.The results showed that the recoveries were all high，and this method was rapid and perfect.

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备注/Memo

第一作者简介：步佳佳（1986-），女，山东济南人，在读硕士，研究方向为果树栽培与生理。E-mail：jjverylovejj@126.com。责任作者：谢江辉（1973-），男，博士，研究员，博士生导师，现主要从事荔枝及香蕉和热带珍稀果树等研究工作。E-mail：xiejianghui@21cn.com。基金项目：海南省自然基金资助项目（311069）；中央级公益性科研院所基本科研业务专项资助项目（sscri201005，1251022011002）；国家香蕉产业技术体系湛江试验站资助项目（CARS-32-12）。收稿日期：2011-02-22

更新日期/Last Update: 2014-08-30