ZHANG Lai-jun,JIA Jing-fen.Effect of Melatonin on the Proliferation and Differentiation of Calli of Scutellaria amoena[J].Northern Horticulture,2013,37(08):106-109.
外源褪黑素对滇黄芩愈伤组织增殖和分化的影响
- Title:
- Effect of Melatonin on the Proliferation and Differentiation of Calli of Scutellaria amoena
- 文章编号:
- 1001-0009(2013)08-0106-04
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以滇黄芩（Scutellaria amoena）为试材，研究了不同浓度外源褪黑素（MEL）对滇黄芩愈伤组织增殖和不定芽分化的影响，并与相应浓度的IAA和NAA作了效果比较。结果表明：在附加0.1、1.0、10.0和100.0 μM MEL的MS培养基上，愈伤组织增殖率均显著高于对照，其作用类似于IAA，而NAA无明显促进作用;低浓度的MEL（0.1、1.0、10.0 μM）有助于愈伤组织的增殖，0.1 μM MEL愈伤组织的增长率最高，达到171%。同时培养基中附加低浓度MEL（0.1或1.0 μM）有助于不定芽分化，1.0 μM的MEL作用下得到最高分化率为30.4%，而高浓度MEL（100.0 μM）则对不定芽分化有抑制作用，与IAA作用相似。关键词：褪黑素；滇黄芩；愈伤组织；芽增殖
- Abstract:
- Taking Scutellaria amoena as material，the effects of different concentrations of exogenous melatonin on the callus proliferation and the differentiation of adventitious buds of Scutellaria amoena were studied.The results showed that the supplement of melatonin at 0.1，1.0，10.0 and 100.0 μM in the MS medium remarkeblly improved the frequency of callus proliferation compared with the control (MS medium without melatonin).Same concentration of indole-3-acetic acid (IAA) in the medium exhibited similar effects，but a-naphthalene acetic acid (NAA) did not promote callus growth.Lower concentration (0.1 or 1.0 μM) of melatonin or IAA added in the medium could increase the differentiation frequency of adventitious buds from callus，however higher level of melatonin or IAA inhibited the bud differentiation.
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备注/Memo
第一作者简介：张来军（1968-），女，甘肃庆阳人，博士，副教授，现主要从事细胞生物学研究等工作。