CHEN Runan,REN Chunyuan,LI He,et al.Exogenous Melatonin Regulates Salt and Alkali Stress Response of Tomato by Inducing SlRR Genes Expression[J].Northern Horticulture,2021,(10):1-8.[doi:10.11937/bfyy.20204002]
外源褪黑素通过诱导SlRR基因表达调控番茄盐碱胁迫响应
- Title:
- Exogenous Melatonin Regulates Salt and Alkali Stress Response of Tomato by Inducing SlRR Genes Expression
- Keywords:
- tomato; MT; salt stress; alkali stress; RR; antioxidant system
- 文献标志码:
- A
- 摘要:
- 以“浙杂205”番茄为试材，通过测定SlRR基因的相对表达量以及番茄幼苗叶片的抗氧化酶系统、活性氧含量、渗透调节物质含量，探讨外源褪黑素是否能有效诱导番茄RR基因的表达以缓解盐碱胁迫对番茄的损伤。结果表明：与对照相比，单独盐、碱处理下Solyc05g0064202（SlRR4）、Solyc10g0797001（SlRR5）、Solyc03g1137202（SlRR6）、Solyc02g0712202（SlRR7）、Solyc01g0655402（SlRR8）、Solyc05g0543902（SlRR9）、Solyc04g0080502（SlRR10）、Solyc12g0103301（SlRR11）的表达水平显著提高，番茄幼苗的超氧化物歧化酶（SOD）、过氧化物酶（POD）、抗坏血酸过氧化物酶（APX）活性、H2O2含量、O?[TX-*4]2含量以及脯氨酸（Pro）含量均显著提高；与单独盐、碱处理相比，褪黑素预处理的番茄幼苗SlRR4、SlRR5的表达量显著上调，SOD、POD、APX活性和Pro含量显著提高，而H2O2含量、O?[TX-*4]2含量显著下降。由此可知，外源褪黑素预处理能够显著诱导SlRR基因的表达以提高抗氧化酶的活性从而减少自由基的产生，提高渗透调节物质含量来维持植物体的渗透平衡，进而缓解盐碱胁迫对番茄的损伤。
- Abstract:
- Taking ‘Zheza 205’ tomato as test material,the relative expression of SlRR gene，the content of antioxidant enzyme system，reactive oxygen species and osmoregulation substances in tomato seedling leaves were measured to explore whether exogenous melatonin could effectively induce tomato RR genes expression to alleviate the damage of salt and alkali stress on tomato.The results showed that compared with the control，the expression of Solyc05g0064202(SlRR4)，Solyc10g0797001(SlRR5)，Solyc03g1137202(SlRR6)，Solyc02g0712202 (SlRR7)，Solyc01g0655402(SlRR8)，Solyc05g0543902(SlRR9)，Solyc04g080502(SlRR10) and Solyc12g0103301 (SlRR11) were significantly increased under salt and alkali treatments.The activities of superoxide dismutase (SOD)，peroxidase (POD)，ascorbate peroxidase (APX) and the content of H2O2,O?[TX-*4]2，and Pro increased significantly.Compared with the single salt and alkali treatment，the expression of SlRR4 and SlRR5 and the activities of SOD，POD，APX and content of Pro were significantly increased after melatonin pretreated tomato seedlings，while the content of H2O2 and O?[TX-*4]2 were decreased significantly.Therefore，exogenous melatonin pretreatment can significantly induce the expression of SlRR genes to improve the activity of antioxidant enzymes，reduce the production of free radicals，increase the content of osmoregulation substances to maintain the osmotic balance of plants，thus alleviating the damage of salt and alkali stress on tomato.
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备注/Memo
第一作者简介：陈如男(1996-)，女，硕士研究生，研究方向为蔬菜生理生态。E-mail:1712662246@ qq.com.责任作者：于高波(1985-)，女，博士，副教授，现主要从事蔬菜生理生态等研究工作。E-mail：yugaobo81@163.com.基金项目：黑龙江省自然基金资助项目(QC2018023)；黑龙江省博士后科研启动资助项目(LBH-Q20052)；黑龙江省归国人才择优资助项目(31301769)；黑龙江八一农垦大学博士启动金资助项目(CVB2012-009)；国家博士后基金资助项目 (2015M581420)；黑龙江省大学生创新资助项目(201910223056)。收稿日期：2020-09-17