LIU He-xia,ZHOU Jian.Study on Callus Induction and Plant Regeneration of Lycoris radiate Herb.[J].Northern Horticulture,2013,37(06):93-96.
石蒜愈伤组织的诱导及其植株再生研究
- Title:
- Study on Callus Induction and Plant Regeneration of Lycoris radiate Herb.
- 文章编号:
- 1001-0009(2013)06-0093-04
- Keywords:
- Lycoris radiate; callus; plant regeneration
- 分类号:
- S 681.903.6
- 文献标志码:
- A
- 摘要:
- 以石蒜为试材，研究了不同激素组合和外植体选择对石蒜愈伤组织诱导及植株再生的影响。结果表明：外植体选择是石蒜愈伤组织诱导的关键因素，3种外植体中，种子胚诱导愈伤组织的效果最好；在MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L培养基中，诱导愈伤率最高可达82.4%。并能较好地诱导愈伤组织发生不定芽，MS+NAA 0.1 mg/L能较好地诱导愈伤组织发生根。石蒜再生植株进行移栽的最佳基质应为腐殖质和珍珠岩以1∶1的比例组成，其成活率为70%。
- Abstract:
- Taking Lycoruis radiate Herb. as material，the effect of some factors such as various hormone concentration，different parts of Lycoruis radiate Herb. on callus induction and plant regeneration were studied.The results showed that the explants selection was the most significant factor of callus induction，and the seed embryos was the best explants for callus induction.The optimal mediums for the callus induction was MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L and the rate reached 82.4%.In the subsequent callus further training，the optimal mediums for the callus induction was MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L，the optimal mediums for the callus induction was NAA 0.1 mg/L.The best matrix for Lycoris radiata regeneration plants was humus and perlite in the proportion of 1∶1 composition，with survival rate 70%.
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备注/Memo
第一作者简介：刘合霞（1984-），女，山东滕州人，在读博士，研究方向为发育植物学。E-mail:liuhexia2010@163.com.