WANG Fei,ZHU Xiao-lan,HE Sha-sha,et al.Preliminary Study on Callus Induction of Corydalis decumbens[J].Northern Horticulture,2013,37(15):125-127.
夏天无愈伤组织诱导的初步研究
- Title:
- Preliminary Study on Callus Induction of Corydalis decumbens
- 文章编号:
- 1001-0009(2013)15-0125-03
- Keywords:
- Corydalis decumbens(Thunb.) Pers.; tissue culture; callus
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以传统中药夏天无为试材，研究了不同消毒方法、不同外植体和不同激素配比对其愈伤组织诱导的影响。结果表明：叶片消毒以01%氯化汞浸泡10 min较好，块茎、叶柄以消毒8 min为好；叶柄作为外植体比块茎、叶片更适合；叶片诱导愈伤组织的最佳激素配比为0.5 mg/L NAA+1.5 mg/L6-BA，诱导率为43.3%，而叶柄诱导愈伤组织的最佳激素配比为1.0 mg/L NAA+1.5 mg/L6-BA，诱导率高达80%。
- Abstract:
- Taking Corydalis decumbens(Thunb.) Pers.as material,the effect of different disinfection methods,explants and hormone proportion on callus induction were studied.The results indicated that the better disinfection treatment was 10 min with 0.1% HgCl2for blade,but 8 min for tuber and leafstalk.As an explants,leafstalk was superior to tuber and blade.The induction rate (43.3%) was the highest in the MS medium with 0.5 mg/L NAA+1.5 mg/L 6-BA for blade,but the induction rate (80.0%) was the highest if the leafstalk was put in the MS medium with 1.0 mg/L NAA+1.5 mg/L 6-BA.
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备注/Memo
第一作者简介：王飞（1980-），男，硕士，讲师，研究方向为药学。
责任作者：黄胜和（1977-），男，博士，研究方向为植物分子生物学与基因工程。E-mail:hsh712@163.com.基金项目：江西省抚州市科技资助项目（20126215）。
收稿日期：2013-04-10