ZHU Hong-fen,SHEN Lan,HUANG Jian,et al.Tissue Culture and Plant Regeneration of Vaccinium ashei?‘Britewell’[J].Northern Horticulture,2012,36(19):105-107.
兔眼蓝莓“灿烂”组织培养与植株再生研究
- Title:
- Tissue Culture and Plant Regeneration of Vaccinium ashei?‘Britewell’
- 文章编号:
- 1001-0009（2012）19-0105-03
- Keywords:
- Vaccinium ashei; tissue culture; plant regeneration
- 分类号:
- S 663.9
- 文献标志码:
- A
- 摘要:
- 以兔眼蓝莓“灿烂”（‘Britewell’）当年生带腋芽半木质化茎段为外植体，研究了不同培养基、激素对兔眼蓝莓“灿烂”植株再生的影响。结果表明：WPM为适宜基本培养基；WPM+（0.5～1.0）mg/L ZT组合是最适的初代幼芽诱导培养基，诱导率达86%以上；WPM+0.1 mg/L TDZ+0.1 mg/L IBA组合是较合适的增殖培养基，增殖率可达5.48，继代培养宜以较低浓度的TDZ（0.02 mg/L和0.05 mg/L）交替使用为好；1/2WPM+1.0 mg/L 6-BA是较理想的生根培养基组合，有效生根率达63.2％。
- Abstract:
- Taking semi-lignified stem with axillary bud of Vaccinium ashei ‘Britewell’ as explants，the effects of the different medias and hormones on plant regeneration were studied.The results showed that WPM was a suitable basic medium.The optimal primary medium for stem with axillary bud was WPM+(0.5～1.0) mg/L ZT，and the induction rate was more than 86%；the suitable proliferation medium was WPM+0.1 mg/L TDZ+0.1 mg/L IBA，the proliferation rate up to 5.48 times.Following the subculture should be at a lower concentration of TDZ (0.02 mg/L and 0.05 mg/L) alternating as well；the rooting medium was 1/2WPM+1.0 mg/L BA，and the effective rooting rate reached 63.2％.
参考文献/References:
[1]中国植物志编辑委员会.中国植物志[M].57卷3分册.北京:科学出版社,1991：75-164. [2]於虹,贺善安,顾姻.我国和世界蓝浆果的发展前景[J].植物资源与环境学报,2001,10(2):52-55. [3]修英涛,常凤英,姜河,等.我国蓝莓(越桔)栽培研究现状及发展措施[J].辽宁农业科学,2003(3)：21-23. [4]杨业正.棉花脱叶剂TDZ简介[J].植物生理学通讯,1989(6):63-64. [5]韩婷婷,孙周平.矮丛蓝莓叶片的愈伤组织诱导及植株再生[J].西北植物学报,2010,30(3):615-620. [6]崔广荣,陆峰,曹华龙,等.蓝莓离体叶片胚状体高效发生及其组织学观察[J].激光生物学报,2008，17(5):599-607. [7]邢瑞丹,刘庆忠,陈新,等.两个蓝莓品种离体叶片不定芽再生体系的建立[J].山东农业科学,2009(5):8-11. [8]Larkin P J，Scowcroft W R.Somaclonal variation-a novel source of variability from cell cultures for plant mprovement[J].Theor Appl Genet,1981,60:197-214. [9]贾春兰,王纪方,金波.蔬菜作物组织培养对染色体倍性变异的影响[J].中国蔬菜,1991(6):17-18. [10]Gtiesbach R J.Selected topics on induced chromosome changes intissue cultured cells[J].Hoil Science,1987,22(6):1024-1026. [11]陈肖英,叶庆生,刘伟.TDZ研究进展(综述)[J].亚热带植物科学,2003,32(3):59-63.
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备注/Memo
第一作者简介：朱宏芬（1974-），女，浙江宁波人，本科，农艺师，现主要从事植物组培研究工作。E-mail：nbnkyzhu@163.com.