SHAO Hong,SUN Rui,ZHANG Li-min,et al.Study on the Technique of Tissue Culture and Rapid Propagation of Aristolochia contorta Bunge[J].Northern Horticulture,2012,36(18):128-130.
北马兜铃组培快繁技术
- Title:
- Study on the Technique of Tissue Culture and Rapid Propagation of Aristolochia contorta Bunge
- 文章编号:
- 1001-0009（2012）18-0128-03
- Keywords:
- Aristolochia contorta Bunge; tissue culture; callus; clumpy bud
- 分类号:
- S 567.23
- 文献标志码:
- B
- 摘要:
- 利用植物组织培养技术对北马兜铃进行快速繁殖，建立北马兜铃植株再生体系。结果表明：愈伤组织诱导的最佳外植体是叶片，在MS+IBA 0.4 mg/L+6-BA 2.0 mg/L+KT 0.5 mg/L培养基中愈伤组织诱导结果较好；愈伤组织分化丛生芽的最佳培养基是MS+IBA0.4 mg/L+6-BA 2.0 mg/L+KT 0.5 mg/L；诱导试管苗生根的佳培养基为MS+IBA 0.4 mg/L+NAA 0.2 mg/L+6-BA 2.0 mg/L+KT 0.5 mg/L。
- Abstract:
- Aristolochia contorta Bunge was used as materials for rapid propagated by plant tissue culture technique，and the plant regeneration system was established.The results showed that the optimal explants for callus induction were leaves，the medium of MS+IBA 0.4 mg/L+6-BA 2.0 mg/L+KT 0.5 mg/L for callus induction was better；the optimal medium for differentiation of clumpy bud was MS+IBA 0.4 mg/L+6-BA 2.0 mg/L+KT 0.5 mg/L；the better medium for root induction was MS+IBA 0.4 mg/L+NAA 0.2 mg/L+6-BA 2.0 mg/L+KT 0.5 mg/L.
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备注/Memo
第一作者简介：邵红(1979-)，女，硕士，讲师，研究方向为植物生理学与植物生物技术。E-mail:jmsshky@126.com.