SHA Wei,CUI Wei,ZHANG Mei-juan.Establishment of Gametophytes Regeneration About Grimmia pilifera[J].Northern Horticulture,2012,36(16):92-94.
毛尖紫萼藓配子体再生体系的建立
- Title:
- Establishment of Gametophytes Regeneration About Grimmia pilifera
- 文章编号:
- 1001-0009(2012)16-0092-03
- Keywords:
- Grimmia pilifera; tissue culture; gametophytes
- 分类号:
- S 603.6
- 文献标志码:
- A
- 摘要:
- 以消毒试验后所得的毛尖紫萼藓原丝体为材料，通过培养，成功的建立了毛尖紫萼藓配子体再生体系。结果表明：MS培养基、低pH能促进毛尖紫萼藓原丝体褐化；葡萄糖能诱导出弱小的配子体；激素对毛尖紫萼藓配子体诱导效果不理想；NH4NO3浓度为2.5 g/L的Prat培养基，适合于毛尖紫萼藓原丝体分化成配子体；将褐化到一定程度的原丝体接种到Prat培养基后，20 d可以生长出较理想的配子体。
- Abstract:
- Taking disinfected protonemata of Grimmia pilifera as material，gametophytes of Grimmia pilifera can be induced successfully through the cultivation.The results indicated that the MS culture medium，low pH could promote protonemata browning；glucose could induce poor gametophytes；hormone could’t induced ideal gametophytes of Grimmia pilifera；Prat culture medium with 2.5 g/L concentration of NH4NO3 was suitable for protonemata of Grimmia pilifera induced into gametophytes.Inoculated some degree of browning protonemata into Prat culture medium，ideal gametophytes could be grown after 20 days.
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备注/Memo
第一作者简介：沙伟（1963-），女，博士，教授，博士生导师，现从事植物学和植物遗传学的教学与科研工作。E-mail:shw1129@163.net.