FANGHui-guo,CHENXi-lin,XINRu-ru,et al.StudyontheTissueCultureandRapidPropagationTechnologyforMelaleucabracteata[J].Northern Horticulture,2012,36(12):146-147.
千层金组培快繁技术研究
- Title:
- StudyontheTissueCultureandRapidPropagationTechnologyforMelaleucabracteata
- 文章编号:
- 1001-0009（2012）12-0146-02
- Keywords:
- Melaleucabracteata; tissureculture; rapidpropagation
- 分类号:
- S792.12
- 文献标志码:
- A
- 摘要:
- 以嫩茎为外殖体,以MS为基本培养基,分别加入不同浓度6-BA、NAA、IBA,研究不同浓度激素及组合对千层金组培快繁的影响。结果表明：诱导芽的最佳培养基为MS+6-BA2.0mg/L+NAA0.2mg/L，诱导率达63.6%；继代培养最佳培养基为MS+6-BA1.0mg/L+NAA0.2mg/L,增殖系数达6.3，且芽苗健壮；壮苗培养的最佳培养基为MS+NAA0.1mg/L,每瓶产苗数达16.2株；最佳生根培养基为1/2MS+NAA0.1mg/L,21d生根率达100%,平均根数达9.6条。采用泥炭土与椰糠体积各半混合基质进行移栽,30d成活率达95.7%,能够满足工厂化苗木生产的要求。
- Abstract:
- Withemployingtenderbineasexplant，MSasbasicmediummixingdifferentdensity6-BA，NAA，IBA，effectofdifferentconcentrationhormoneanditscombinationontissuecultureandrapidpropagationofMelaleucabracteatawerestudied.TheresultsindicatedthattheoptimalmediumofinducingsproutwasMS+6-BA2.0mg/L+NAA0.2mg/L，andtheinductionrateachieved63.6%；theoptimalmediumofsuccessiveproliferationsproutwasMS+6-BA1.0mg/L+NAA0.2mg/L，theproliferationcoefficientachieved6.3withsproutingoodhealth；theoptimalmediumofseedlingcultivationwasMS+NAA0.1mg/L，andthenumberofseedlingpertrainingbottleachieved16.2；theoptimalmediumofroottrainingwas1/2MS+NAA0.1mg/L，therootingratein21dachieved100%，andthemeannumberofrootsachieved9.6.Usingpeatsoilmixingcoconutchaffhalfinhalfinvolumefortransplanting，thesurvivalratein30dachieved95.7%，itcouldfitfactoryseedlingsproductionrequirements.
参考文献/References:
[1]吴维坚,林加根,鞠玉栋,等.千层金组培快繁技术研究[J].中国农学通报,2010,26(18):247-250.
[2]李放娟,柳江海,陈喜林,等.龙翅悬秋海棠的组织培养及快速繁殖[J].广东园林,2005(增刊):26-27.
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备注/Memo
第一作者简介：方惠国(1975-)，男，本科，工程师，现从事城市景观园林与林业规划设计工作。E-mail:xilinchen2008@sina.com。
责任作者：陈喜林（1964-），男，本科，高级工程师，现主要从事园林植物组培技术工作。
收稿日期：2012-03-15