[1]武鹏,方锋学,黄如葵,等.苦瓜离体再生体系建立的研究[J].北方园艺,2012,36(02):124-126.
　WU Peng,FANG Feng-xue,HUANG Ru-kui,et al.Study on the Establishment of in vitro Regeneration System in Momordica charontia L.[J].Northern Horticulture,2012,36(02):124-126.

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苦瓜离体再生体系建立的研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷期数: 2012年02期页码: 124-126 栏目: 出版日期: 2012-02-16

Title:: Study on the Establishment of in vitro Regeneration System in Momordica charontia L.

文章编号:: 1001-0009（2012）02-0124-03

作者:: 武鹏¹; 方锋学²; 黄如葵³; 黄熊娟³; 陈小凤³; 1.广西农业科学院广西现代农业科技示范园,广西南宁 530007；
2.广西农业科学院甘蔗研究所,广西南宁 530007；
3.广西农业科学院蔬菜研究所,广西南宁 530007

Author(s):: WU Peng¹; FANG Feng-xue²; HUANG Ru-kui³; HUANG Xiong-juan³; CHEN Xiao-feng³; 1.Guangxi Demonstration Park of Modern Agricultural Science and Technology,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007;
2.Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007;
3.Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007

关键词:: 苦瓜; 离体培养; 愈伤组织; 不定芽

Keywords:: Momordica charantia.L; in vitro culture; callus; adventitious bud

分类号:: S 6425036

文献标志码:: A

摘要:: 以MS为基本培养基，附加不同浓度的激素组合进行苦瓜离体再生体系的研究。结果表明：种子去壳洗净浸泡5 min,用70%酒精浸泡1 min,洗净后01% HgCl2消毒10 min的灭菌效果最好；通过愈伤组织诱导不定芽没有取得成功；利用无菌苗不同部位直接诱导不定芽，子叶节效果最好，最高诱导率达到100%,最适宜诱导培养基是MS+6-BA 2 mg/L+IBA 002 mg/L和MS+6-BA 4 mg/L+IBA 001 mg/L；最适生根培养基为1/2MS+IBA 02 mg/L。

Abstract:: In vitro regeneration system of Momordica charantia L.based on MS medium with various concentration of hormone combinations were studied.The results showed that the most effective disinfection was reached when the clean peeled seeds were socked for 5 min,70% ethanol for 1 min and rinsing before HgCl2treatment for 10 min.Adventitious buds induction with callus was failed,while cotyledonary node reached 100% induction rate among all the parts of the sterile seedling and the optimum induction medium were MS+6-BA 2 mg/L+IBA 002 mg/L and MS+6-BA 4 mg/L+IBA 001 mg/L and the optimum root medium was 1/2MS+IBA 02 mg/L.

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备注/Memo

第一作者简介：武鹏（1978-），男，硕士，助理研究员，现从事蔬菜育种与栽培研究工作。E-mail:wupeng@gxaas.net。

基金项目：广西农业科学院基本科研业务专项资助项目（200815(基) ）。

收稿日期：2011-11-10

更新日期/Last Update: 2014-08-24