[1]马秀明,胡云超,缪军,等.亚洲百合‘穿梭’组培快繁体系的建立[J].北方园艺,2024,(4):42-48.[doi:10.11937/bfyy.20233397]
　MA Xiuming,HU Yunchao,MIAO Jun,et al.Establishment of Lilium ‘Trensor’ in vitro Rapid Propagation System[J].Northern Horticulture,2024,(4):42-48.[doi:10.11937/bfyy.20233397]
点击复制

亚洲百合‘穿梭’组培快繁体系的建立

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2024年4 页码: 42-48 栏目: 出版日期: 2024-02-29
Title:
Establishment of Lilium ‘Trensor’ in vitro Rapid Propagation System
文章编号:
1001-0009(2024)04-0042-07
作者:
马秀明1; 胡云超2; 缪军1; 张庶1; 王俊峰1; 石少川1
（1.山东省农业科学院 蔬菜研究所，山东 济南 250100；2.济南中科绿色生物工程有限公司，山东 济南 250100）
Author(s):
MA Xiuming1; HU Yunchao2; MIAO Jun1; ZHANG Shu1; WANG Junfeng1; SHI Shaochuan1
(1.Institute of Vegetable，Shandong Academy of Agricultural Sciences，Jinan，Shandong 250100；2.Jinan Zhongke Green Biological Engineering Co.Ltd.，Jinan，Shandong 250100)
关键词:
植物激素; 组织培养; 快繁体系
Keywords:
plant hormone; tissue culture; rapid propagation system
分类号:
S 682.29
DOI:
10.11937/bfyy.20233397
文献标志码:
A
摘要:
以亚洲百合‘穿梭’为试材，采用植物组织培养的方法，研究了不同浓度激素对百合鳞片诱导、不定芽增殖、小鳞茎生根培养的影响，并筛选出适宜百合组培苗生长的移栽基质，以期为亚洲百合‘穿梭’的组培快繁体系的建立提供参考依据。结果表明：百合鳞片最佳诱导培养基为MS+0.5 mg·L-1 NAA+0.5 mg·L-1 6-BA+30 g·L-1蔗糖+6 g·L-1琼脂，诱导率最高可达74.44%，显著高于其他处理；最佳增殖培养基为MS+0.1 mg·L-1 NAA+1.0 mg·L-1 6-BA+30 g·L-1蔗糖+6 g·L-1琼脂，不定芽增殖数量最多，增殖系数为3.27；适合小鳞茎生根培养基为1/2MS+0.1 mg·L-1 NAA+0.3 mg·L-1 IBA+40 g·L-1蔗糖+6 g·L-1琼脂，平均生根数量为14.33，是其他处理的1.15~2.02倍；移栽后组培苗在草炭与珍珠岩为1∶1的基质配比中成活率高达87.50%，且组培苗长势最好。
Abstract:
The ‘Trensor’ of Asiatic lily was used as the test material.The effects of different concentrations of hormones on the scale induction，adventitious bud proliferation and bulb rooting culture of Asiatic lily were studied by the method of plant tissue culture,and the effects of different transplanting substrates on the growth of lilium tissue culture seedlings were studied，in order to provide reference for the establishment of ‘Trensor’ tissue culture rapid propagation system of Lilium asiatica.The results showed that the optimal medium for lilium scale induction was MS+0.5 mg·L-1 NAA+0.5 mg·L-1 6-BA+30 g·L-1 sucrose+6 g·L-1 agar，and the highest induction rate was 74.44%，which was higher than other treatments.The best proliferation medium was MS+0.1 mg·L-1 NAA+1.0 mg·L-1 6-BA+30 g·L-1 sucrose+6 g·L-1 agar，and the number of advective buds was the highest with a proliferation coefficient of 3.27.The suitable rooting medium was 1/2MS+0.1 mg·L-1 NAA+0.3 mg·L-1 IBA+40 g·L-1 sucrose+6 g·L-1 agar，and the average rooting number was 14.33.It was 1.15-2.02 times more than other treatments.After transplanting，the survival rate of tissue culture seedlings was 87.50% in the matrix ratio of peat and perlite was 1∶1，and the growth of tissue culture seedlings was the best.

参考文献/References:

［1］刘晶荣，代思奇，刘才磊，等.丽江百合组织培养及再生体系建立［J］.安徽农业科学，2023，51(9)：38-41.［2］李晴，石雨荷，朱珏，等.药食同源百合的资源分布与现代研究进展［J］.中国野生植物资源，2023，42(3)：87-95.［3］胡新颖，李雪艳，王伟东，等.不同消毒处理对大花卷丹百合种子发芽的影响［J］.辽宁农业科学，2022(2)：23-26.［4］刘玉琴，张洁，林智敏.综合处理对百合鳞片扦插诱导小鳞茎的影响［J］.东南园艺，2021，9(5)：41-46.［5］ASKARI N，VISSER R G F.The role of scale explants in the growth of regenerating lily bulblets in vitro［J］.Plant Cell，Tissue and Organ Culture (PCTOC)，2022,149(3):589-598.［6］张彩玲，沈洪宽，肖子恒，等.甜百合的组织培养初步研究［J］.中国林副特产，2023(1)：6-8.［7］邢景景，刘国锋，曾凤，等.淡黄花百合的引种驯化与繁殖栽培技术［J］.中国野生植物资源，2019，38(5)：88-92.［8］李论，李丽梅，龚意辉，等.龙牙百合快速繁殖与脱毒技术分析［J］.现代园艺，2022，45(7)：71-73.［9］GHEWARE K M,KAUSHIK K,GANGWAR R，et al.The effect of different concentrations of GA3 and NAA on growth and flowering of Asiatic Lily (Lilium × asiatica) cv.Litouwen under naturally ventilated polyhouse conditions of prayagraj［J］.International Journal of Environment and Climate Change,2022(3)：1196-1201.［10］裴怀弟，林玉红，李淑洁，等.兰州百合组培小鳞茎诱导技术研究［J］.甘肃农业科技，2019(7)：29-32.［11］祁先宇，张文亮，杨盼盼，等.赏食兼用百合京鹤高效组培再生体系的优化［J］.中国蔬菜，2022(8)：57-62.［12］AZER〖AKI·〗 F N，ZTRK G.Microbulb and plantlet formation of a native bulbous flower，Lilium monodelphum M.Bieb，var.Armenum，through tissue culture propagation［J］.Biotechnology Reports,2021,32:e00665.［13］任明波，杨毅，刘杰，等.南川百合资源现状及组培快繁技术研究［J］.西南大学学报(自然科学版)，2019，41(11)：19-24.［14］崔光芬，段青，杜文文，等.食用百合生态适应性及离体繁殖差异［J］.江苏农业科学，2019，47(22)：196-199.［15］RAHBAR F G,VAZIRI A,ASIL M H，et al.Effects of humic acid on antioxidant defense system and senescence-related gene expression in leaves of longiflorum×asiatic of Lilies (LA Lilium Hybrid)［J］.Journal of Soil Science and Plant Nutrition,2023,23(3):3500-3507.［16］胡彬杰，白瑞琴，尹晶晶，等.沂水食用百合组培快繁体系的构建［J］.北方农业学报，2021，49(3)：90-95.［17］樊敏，唐亚玲，王莉，等.兰州百合不同外植体植物组织培养［J］.农业与技术，2021，41(18)：26-28.［18］王强，李本润，张梅，等.汶川地区细叶百合栽培种的快繁体系探索［J］.农业与技术，2020，40(10)：10-14.［19］WU Y，MA Y D，LI Y,et al.Plantlet regeneration from primary callus cultures of Lilium brownii F.E.Br.ex Miellez var.giganteum G.Y.Li & Z.H.Chen，a rare bulbous germplasm［J］.In Vitro Cellular & Developmental Biology-Plant,2019,55(1):44-59.［20］李茂娟，廖祯妮，邓少华，等.百合新品种引种筛选及组培快繁技术研究［J］.中国农学通报，2019，35(22)：65-70.［21］张悦，李爱民，张正海，等.优质观赏百合品种“木门”组织培养研究［J］.北方园艺，2014(18)：112-114.［22］YOUSSEF N M,SHAABAN S A,GHAREEB Z F,et al.In vitro bulb formation of direct and indirect regeneration of Lilium orientalis cv.“Starfighter” plants［J］.Bulletin of the National Research Centre,2019,43(1):211.［23］段四喜，徐春莲，汤王外，等.3种因素对兰州百合不定芽诱导的影响［J］.江西农业学报，2017，29(12)：47-52,58.［24］杜帅，李丹，杜喜春，等.绿花百合组培快繁技术研究［J］.现代园艺，2014(8)：12-13.［25］王政，孙宇科，尚文倩，等.LED不同照光方式对百合组培苗生长的影响［J］.北方园艺，2022(6)：70-76.［26］SAHOO M R，DEVI M P，DASGUPTA M,et al.An efficient protocol for in vitro regeneration and conservation of Shirui lily (Lilium mackliniae Sealy)：A lab-to-land approach to save the rare endangered Asiatic lily species［J］.In Vitro Cellular & Developmental Biology-Plant,2018,54(6):701-710.［27］李雪艳，胡新颖，王伟东，等.无病毒百合‘Sorbonne’试管内小鳞茎直接再生体系建立［J］.北方园艺，2021(9)：80-86.［28］朱岩，王红，代宁，等.大花卷丹百合组培快繁技术研究［J］.甘肃农业科技，2022，53(9)：76-80.［29］马金萍，王帆.兰州百合组培快繁技术研究［J］.现代农业科技，2023(8)：69-72.［30］陈少鹏，张剑锋.不同栽培基质对毛百合组培苗移栽的影响［J］.黑龙江农业科学，2016(5)：81-82.［31］彭伯阳，白瑞琴，吕艳芳，等.两种食用百合离体快繁体系的建立［J］.北方农业学报，2022，50(6)：88-97.［32］任君芳，张利，刘建霞，等.东方百合‘Vivian’高效离体快繁体系的建立［J］.四川林业科技，2020，41(6)：84-88.［33］蔡宣梅，郭文杰，张洁，等.“罗宾娜”百合组培快繁体系的建立［J］.福建热作科技，2017，42(1)：36-38.

相似文献/References:

[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]曹 虹,金荣荣.三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究[J].北方园艺,2014,38(13):106.
　CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(4):106.
[4]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
　ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(4):80.
[5]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
　WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(4):84.
[6]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
　ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(4):101.
[7]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
　DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(4):89.
[8]赵丽莉,徐芳芳,荀 洋,等.桔梗花药愈伤组织诱导培养基筛选[J].北方园艺,2014,38(01):95.
　ZHAO Li-li,XU Fang-fang,XUN Yang,et al.Screening of Callus Induction Medium of Platycodon grandiflorumAnther[J].Northern Horticulture,2014,38(4):95.
[9]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
　LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(4):106.
[10]陈丽文,杨利平,荣薏,等.金心也门铁工厂化育苗技术研究[J].北方园艺,2013,37(13):138.
　CHEN Liwen,YANG Li-ping,RONG Yi,et al.Study on the Techniques of Industrialized Seedling Culture of Draceana arborea[J].Northern Horticulture,2013,37(4):138.
[11]黄 颖,胡 磊,谷 晴,等.“大马士革”玫瑰茎尖组培快繁技术研究[J].北方园艺,2014,38(03):104.
　HUANG Ying,HU Lei,GU Qing,et al.Study on Tissue Culture and Rapid Propagation on Stem Tip of Rosa damascus[J].Northern Horticulture,2014,38(4):104.
[12]金建邦,祝遵凌.桦木科植物组织培养研究进展[J].北方园艺,2013,37(23):202.
　JIN Jian-bang,ZHU Zun-ling.Research Progress of Tissue Culture of Betulaceae[J].Northern Horticulture,2013,37(4):202.
[13]王萍,沈晓婷,王罡,等.植物激素对黄瓜愈伤组织形成与芽分化的影响[J].北方园艺,2013,37(24):84.
　WANG Ping,SHEN Xiao-ting,WANG Gang,et al.Effects of Phytohormones on Callus Formation and Buds Differentiation of Cucumber[J].Northern Horticulture,2013,37(4):84.
[14]宋英今,季　静,刘海学,等.生菜子叶组织培养及全息现象的研究[J].北方园艺,2007,31(01):0.[doi:10.11937/bfyy.200701065]
　SONG Ying-jin,JI Jing,LIU Hai-xue,et al.Research on Tissue culture and the Holographic Phenomenon of Lactuca sativa var. capitata L[J].Northern Horticulture,2007,31(4):0.[doi:10.11937/bfyy.200701065]
[15]包晗,张芮,张美玲,等.甜叶菊叶片外植体再生体系的建立[J].北方园艺,2018,42(06):16.[doi:10.11937/bfyy.20173097]
　BAO Han,ZHANG Rui,ZHANG Meiling,et al.Establishment of a Regeneration System From Leaf Explant of Stevia rebaudiana[J].Northern Horticulture,2018,42(4):16.[doi:10.11937/bfyy.20173097]
[16]姜良珍,马建华,黄鹏,等.基于气生根的雷公藤植株的快速繁殖技术[J].北方园艺,2019,43(15):119.[doi:10.11937/bfyy.20184075]
　JIANG Liangzhen,MA Jianhua,HUANG Peng,et al.Tissue Culture and Rapid Propagation of Tripterygium wilfordii Seedlings Based on Air Rooting[J].Northern Horticulture,2019,43(4):119.[doi:10.11937/bfyy.20184075]
[17]符真珠,袁欣,王爱科,等.彩叶楝树组织培养技术[J].北方园艺,2020,44(13):71.[doi:10.11937/bfyy.20192882]
　FU Zhenzhu,YUAN Xin,WANG Aike,et al.Tissue Culture of Colored Tree Cultivar Melia azedarach[J].Northern Horticulture,2020,44(4):71.[doi:10.11937/bfyy.20192882]
[18]许丁帆,刘艳军,王赵囡,等.绒毛白蜡组培快繁体系的建立[J].北方园艺,2021,(19):78.[doi:10.11937/bfyy.20211382]
　XU Dingfan,LIU Yanjun,WANG Zhaonan,et al.Establishment of Tissue Culture and Rapid Propagation System in Fraxinus velutina[J].Northern Horticulture,2021,(4):78.[doi:10.11937/bfyy.20211382]

备注/Memo

第一作者简介：马秀明(1991-)，男，硕士，助理研究员，现主要从事特色蔬菜脱毒组培快繁等研究工作。E-mail：15275381908@163.com.责任作者：石少川（1986-），男，博士，副研究员，现主要从事蔬菜和花卉分子育种等研究工作。E-mail：shaochuan0066@126.com.基金项目：山东省农业科学院农业科技创新工程资助项目（CXGC2023F08）。收稿日期：2023-10-08

更新日期/Last Update: 2024-03-15