ZHAO Jie,HOU Yuqi,ZHENG Wei,et al.Study on in vitro Rapid Propagation of Elsholtzia bodinieri[J].Northern Horticulture,2023,(23):60-67.[doi:10.11937/bfyy.20231147]
凤尾茶的离体快繁技术研究
- Title:
- Study on in vitro Rapid Propagation of Elsholtzia bodinieri
- 文章编号:
- 1001-0009(2023)23-0060-08
- 分类号:
- S 681.9
- 文献标志码:
- A
- 摘要:
- 以凤尾茶的茎尖作为外植体，采用不同浓度的植物生长调节剂进行组培试验，研究了不同消毒处理、培养基种类及激素浓度和移栽基质等因素对凤尾茶初代培养、继代增殖、生根培养及炼苗移栽的影响，以期为凤尾茶的离体快繁、离体诱变和遗传转化等研究提供参考依据。结果表明：凤尾茶茎尖的最适灭菌方法为75%酒精30 s+0.1% HgCl2灭菌6 min，存活率达76.67%；最适初代培养基为MS+6-BA 1.0 mg·L-1+IAA 0.10 mg·L-1，诱导率高达 88.89%；最适增殖培养基为MS+6-BA 0.3 mg·L-1+NAA 0.05 mg·L-1，〖JP3〗增殖系数为12.91；最佳生根培养基为1/2 MS+IBA 1.00 mg·L-1，生根率可达97.78%；开盖炼苗4 d后，移栽至腐殖土珍珠岩泥炭=1∶1∶1混合基质中，成活率达91.11%。
- Abstract:
- The stem tips of Elsholtzia bodinieri were used as explants and different concentrations of plant growth regulators were used in tissue culture experiments.The effects of different sterilization treatments,media types，hormone concentrations and transplanting substrates on the initial culture，subculture proliferation，rooting culture and seedling transplanting of Elsholtzia bodinieri were studied.In order to lay a foundation for in vitro propagation，in vitro mutagenesis and genetic transformation of Elsholtzia bodinieri.The results showed that the optimum sterilization method for the stem tips of Elsholtzia bodinieri was 75% alcohol for 30 seconds＋0.1% HgCl2 for 6 minutes，and the survival rate was 76.67%.The optimum primary culture medium was MS＋6-BA 1.0 mg·L-1＋IAA 0.10 mg·L-1，and the induction rate was up to 88.89%.The optimum proliferation medium was MS＋6-BA 0.3 mg·L-1＋NAA 0.05 mg·L-1，the proliferation coefficient was 12.91.The best rooting medium was 1/2MS＋IBA 1.00 mg·L-1，with a rooting rate of 97.78%.After 4 days of open cover，the seedlings were transplanted into a mixed substrate of 1 humus soil∶1 perlite∶1 peat，with a survival rate of 91.11%.
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备注/Memo
第一作者简介：赵洁（1998-)，女，硕士研究生，研究方向为园林植物资源及应用。E-mail:310374853@qq.com.责任作者：徐晓丹（1984-)，女，博士，副教授，现主要从事园林植物资源及应用等研究工作。E-mail:125128370@qq.com.基金项目：国家自然科学基金资助项目（31660228，32060691）。收稿日期：2023-04-04