SHI Wanyuan,XU Hongda,WEI Zhuo,et al.‘Xuxiang’ Kiwifruit Leaf Induction Seedling Propagation Technology[J].Northern Horticulture,2021,(24):16-22.[doi:10.11937/bfyy.20211694]
‘徐香’猕猴桃叶片诱导成苗扩繁技术
- Title:
- ‘Xuxiang’ Kiwifruit Leaf Induction Seedling Propagation Technology
- Keywords:
- ‘Xuxiang’ kiwifruit; tissue culture; callus; adventitious buds; rooting
- 文献标志码:
- A
- 摘要:
- 以‘徐香’猕猴桃叶片为试材，采用植物离体组培技术，研究了在利用叶片组培过程中消毒时间和不同浓度的植物激素组合在不同阶段的影响，以期为缩短‘徐香’猕猴桃组培育苗周期提供参考依据。结果表明：叶片消毒、培养基筛选、诱导愈伤组织、诱导不定芽、诱导生根、炼苗移栽6个过程，需70 d左右。‘徐香’猕猴桃叶片采集后，需用清水洗涤叶片表皮30~45 min，消毒效果最佳为在无菌工作台中用75％的C2H5OH消毒20 s，再用0.1％的HgCl2溶液消毒5 min，幼苗成活率为86.7％；叶片在MS+蔗糖 30 g·L-1+琼脂 4.5 g·L-1+TDZ 2.0 mg·L-1+IBA 0.5 mg·L-1培养基中培养35 d诱导愈伤组织效果最佳，愈伤率为100％；愈伤组织诱导分化不定芽最佳培养基为MS+蔗糖 30 g·L-1+琼脂 4.5 g·L-1+NAA 0.3 mg·L-1+6-BA 3.0 mg·L-1，分化率为93.33%±2.66％，平均芽数(16.97±0.79）个；不定芽诱导生根最佳培养基为1/2MS+蔗糖 30 g·L-1+琼脂 4.5 g·L-1+IBA 0.8 mg·L-1+AC 0.25 g·L-1，生根率为91.37%±6.35％，平均根长(7.24±1.04)cm，平均生根数(14.46±1.56)条；炼苗3~5 d后，移栽至成分为园土、腐殖土、蛭石、珍珠岩、草木灰（3∶1∶1∶1∶1）的土壤中，植株成活率较高，长势较好。用该组培技术，可在短期内得到大量不携带病虫害的‘徐香’种苗。
- Abstract:
- Taking the leaves of ‘Xuxiang’ kiwifruit as the test material，using the in vitro plant tissue culture technology，the effects of disinfection time and different concentrations of phytohormone combinations at different stages in the process of using leaf tissue culture were studied，in order to provide reference for shortening ‘Xuxiang’ tissue culture.The results showed that it took about 70 days for the 6 processes including leaf disinfection，medium selection，callus induction，adventitious bud induction，rooting induction，and seedling transplanting.After collecting the leaves of ‘Xuxiang’ kiwifruit，clean water was needed to wash the epidermis of the leaves for 30-60 minutes.The best disinfection effect was as follows,sterilize with 75% C2H5OH in a sterile workbench for 20 seconds，and then use 0.1% HgCl2 solution for 5 minutes.The survival rate of seedlings was 86.7%.The leaves were cultured in MS+sugar 30 g·L-1+agar 4.5 g·L-1+TDZ 2.0 mg·L-1+IBA 0.5 mg·L-1 medium for 35 days to obtain the best callus induction effect，and the callus rate was 100%.The optimal medium for callus induction and differentiation of adventitious buds was MS+sugar 30 g·L-1+agar 4.5 g·L-1+NAA 0.3 mg·L-1+6-BA 3.0 mg·L-1，with a differentiation rate of 93.33%±2.66% and an average number of buds of 16.97±0.79.The best rooting medium was 1/2MS+sugar 30 g·L-1+agar 4.5 g·L-1+IBA 0.8 mg·L-1+AC 0.25 g·L-1，the rooting rate was 91.37%±6.35%；the average root length was (7.24±1.04) cm，the average number of roots was 14.46±1.56.After the seedlings were refined for 3-5 days，they were transplanted into soil composed of garden soil，humus soil，vermiculite，perlite，and plant ash (3∶1∶1∶1∶1∶1).The plant survival rate was higher and the growth was better.With tissue culture technology，a large number of ‘Xuxiang’ seedlings that do not carry pests can be obtainedin a short period of time.
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备注/Memo
第一作者简介：师万源（1995-），男，硕士研究生，研究方向为林木遗传育种和植物生物技术。E-mail：877595031@qq.com.责任作者：张汉尧（1975-），男，博士，教授，博士生导师，现主要从事植物和微生物遗传育种等研究工作。E-mail：hanyaoz@163.com.基金项目：国家自然科学基金资助项目（31760450）；云南省教育厅科学研究基金资助项目（2019Y126）；大学生创新创业训练资助项目（201810677002）。收稿日期：2021-04-19