ZHU Xiaoqin,ZHANG Qi,ZHENG Aizhen,et al.Isolation and Identification of Strawberry Leaf Spot Disease in Shangqiu[J].Northern Horticulture,2021,(17):38-43.[doi:10.11937/bfyy.20204762]
商丘地区草莓叶斑病的分离与鉴定
- Title:
- Isolation and Identification of Strawberry Leaf Spot Disease in Shangqiu
- 文献标志码:
- A
- 摘要:
- 以商丘双八镇草莓为试材，〖JP+2〗采用组织分离法，研究了引起草莓叶斑病的病原菌鉴定，以期为草莓种植过程中叶斑病防治提供参考依据。结果表明：引起草莓叶斑病的病原菌是一株真菌，编号为SQZQ-06，通过柯赫法则对该菌株的致病性进行测定，发现该菌株可以导致草莓叶片出现斑状病害特征。PCR扩增SQZQ-06菌株18S rRNA基因得到长度为589 bp的序列片段，将测定结果在NCBI网站进行比对并作系统进化树分析，综合该真菌的形态学特征后确定SQZQ-06为斑污拟盘多毛孢Pestalotiopsis maculans。
- Abstract:
- Taking the leaves of strawberry in Shuangba town,Shangqiu as material,the pathogen of strawberry leaf spot disease by tissue isolation was investigated，in order to provide a reference for the control of strawberry leaf spot disease.The results showed that a fungus SQZQ-06 from strawberry leaf spot in Shuangba town,Shangqiu,was identified as Pestalotiopsis maculans.The pathogenicity of the strain was determined by Coch rule,and it was found that the strain could lead to the necrosis of the strawberry leaves.The 18S rRNA gene of SQZQ-06 was amplified by PCR for molecular identification,and the length was 589 bp.Blast result in NCBI and phylogenetic tree analysis suggested that SQZQ-06 was Pestalotiopsis maculans.According to the morphological characteristics and molecular identification of the fungus,SQZQ-06 was identified as Pestalotiopsis maculans.
参考文献/References:
[1]沙春艳.北方温室草莓病害的发生与防治技术[J].黑龙江农业科学,2012(3):159-160.[2]宋永海.大棚草莓病害的发病症状及防治对策[J].现代农业,2017(5):53.[3]王振华,张建坤,龚国祥,等.草莓枯萎病菌的分离与鉴定[J].湖北农业科学,2014,53(14):3297-3299.[4]赵景楠,马喆,刘正坪,等.草莓拟盘多毛孢叶斑病的病原菌[J].菌物学报,2016,35(1):114-120.[5]周德庆.微生物学实验教程[M].3版.北京：高等教育出版社，2013.[6]吴发红,黄东益.几种真菌DNA提取方法的比较[J].中国农学通报,2009,25(8):62-64.[7]江秀均,柴建萍,占世丰,等.桑褐斑病病原菌的分离及以离体叶片接种测定致病性的方法[J].蚕业科学,2015，41(1):37-41.[8]赵玳琳,卯婷婷,赵兴丽,等.草莓炭疽菌初期侵染过程显微观察[J].南方农业学报,2016,47(7):1140-1145.[9]葛起新.中国真菌志(第三十八卷)[M].北京：科学出版社，2009.[10]宋利沙,韦继光,云朝光,等.广西良凤江国家森林公园植物内生拟盘多毛孢属真菌种类多样性调查[J].林业科学，2016,52(7)：87-95.[11]MAHARACHCHIKUMBURA S S,GUO L D,CHUKEATIROTE E,et al.Pestalotiopsis-morphology，phylogeny，biochemistry and diversity[J].Fungal Diversity，2011，50(1):167-187.[12]HOPKINS K E，MCQUILKEN M P.Characteristics of Pestalotiopsis associated with hardy ornamental plants in the UK[J].European Journal of Plant Pathology,2000，106:77-85.[13]葛起新,徐同,孙小桉,等.山茶灰斑病病原菌斑污拟盘多毛孢的研究[J].真菌学报,1993(3):200-204.[14]耿文龙.草莓褐色叶斑病病原菌生物学特性及其室内药剂筛选[C].青岛：中国植物病理学会2012年学术年会,2012.[15]元超，李刚，王海英，等.地衣内生真菌Pestalotiopsis sp.次生代谢产物[J].菌物学报，2018,37(2):237-245.
相似文献/References:
[1]杨 爽,李海鹏,杨培鑑,等.微生物菌肥对草莓光合特性的影响[J].北方园艺,2014,38(11):165.
YANG Shuang,LI Hai-peng,YANG Pei-jian,et al.Effect of Biofertilizer on Photosynthesis Characters of Strawberries[J].Northern Horticulture,2014,38(17):165.
[2]杨 爽,李海鹏,杨培鑑,等.不同草莓品种对白粉病的抗性调查[J].北方园艺,2014,38(10):104.
YANG Shuang,LI Hai-peng,YANG Pei-jian,et al.Survey on the Resistance to the Powdery Mildew of Different Strawberries Cultivars[J].Northern Horticulture,2014,38(17):104.
[3]李 邵,田 婧,鲁少尉,等.设施草莓高架分层育苗系统育苗效果研究[J].北方园艺,2014,38(14):41.
LI Shao,TIAN Jing,LU Shao-wei,et al.Study on Nursery Effect of an Elevated and Layered Seedling Raising System for Greenhouse Strawberry[J].Northern Horticulture,2014,38(17):41.
[4]刘万达,张丙秀,魏媛媛,等.草莓DHAR基因密码子偏好性分析[J].北方园艺,2014,38(14):92.
LIU Wan-da,ZHANG Bing-xiu,WEI Yuan-yuan,et al.Analysis of Codon Bias of DHAR Gene in Strawberry[J].Northern Horticulture,2014,38(17):92.
[5]梁新安,梁芳芳.高品质草莓日光温室栽培技术[J].北方园艺,2013,37(13):56.
LIANG Xin-an,LIANG Fang-fang.High Quality Strawberry Cultivation Techniques in Solar Greenhouse[J].Northern Horticulture,2013,37(17):56.
[6]李星月,刘奇志,李贺勤,等.大棚草莓棉蚜分布规律及其生态调控意义研究[J].北方园艺,2014,38(02):124.
LI Xing-yue,LIU Qi-zhi,LI He-qin,et al.Research on Spatial Distribution and Ecological Control of Aphis gossypii Glover in Greenhouse Strawberry[J].Northern Horticulture,2014,38(17):124.
[7]葛水莲,焦云红,陈建中,等.苦苣菜提取物在草莓采后保鲜中的应用研究[J].北方园艺,2013,37(10):130.
GE Shui-lian,JIAO Yun-hong,CHEN Jian-zhong,et al.Application of Extracts from Sonchus oleraceus on Preservation of Postharvested Strawberry[J].Northern Horticulture,2013,37(17):130.
[8]赵菊莲.丛枝菌根真菌诱导草莓枯萎病抗性机理研究[J].北方园艺,2013,37(17):115.
ZHAO Ju-lian.Study on Mechanism of Strawberry Fusarium Wilt Resistance Induced by Arbuscular Mycorrhizae Fungus[J].Northern Horticulture,2013,37(17):115.
[9]万 红,陶 磅,孔令明,等.草莓有机生态型盆栽基质研究[J].北方园艺,2014,38(08):149.
WAN Hong,TAO Pang,KONG Ling-ming,et al.Research on Substrate for Eco-organic Pot Culture of Strawberry[J].Northern Horticulture,2014,38(17):149.
[10]李建龙,王永和,成太祥,等.蚯蚓粪改良剂对草莓产量和品质的影响[J].北方园艺,2014,38(06):163.
LI Jian-long,WANG Yong-he,CHENG Tai-xiang,et al.Effect of Earthworm-cast Modifier on the Yield and Quality of Strawberry[J].Northern Horticulture,2014,38(17):163.
备注/Memo
第一作者简介：朱晓琴（1975-），女，博士，副教授，现主要从事植物与微生物互作等研究工作。E-mail:zhuxq320@126.com.责任作者：裴冬丽（1971-）,女，博士，教授，现主要从事材料与微生物互作等研究工作。E-mail:peidongli@126.com.基金项目：国家自然科学基金资助项目（31902066）；河南省自然科学基金资助项目（182300410058）；河南省高校科技创新团队（21IRTSTHN025）。收稿日期：2020-06-27