SONG Peng,DING Yanfen,XU Zihan,et al.Studies on Extraction,Stability and Composition of Anthocyanins From Leaves of Euonymus maackii[J].Northern Horticulture,2019,43(24):81-87.[doi:10.11937/bfyy.20191086]
丝棉木叶片花色苷的提取、稳定性和成分研究
- Title:
- Studies on Extraction,Stability and Composition of Anthocyanins From Leaves of Euonymus maackii
- Keywords:
- leaves of E.maackii; anthocyanins; extraction; stability; components
- 文献标志码:
- A
- 摘要:
- 以秋冬季丝棉木的红色叶片为材料，研究叶片中花色苷的提取工艺和不同条件下的稳定性，并采用高效液相色谱法对其成分做出鉴定。结果表明:丝棉木叶片中花色苷的最佳提取方法为以90%甲醇-0.1 mol?L-1盐酸作为提取液，提取温度为60 ℃，提取时间为3 h，料液比为1∶40 g?mL-1。花色苷对日光敏感，在酸性条件下稳定，60 ℃以下比较稳定，Pb2+、Fe2+、Fe3+和Cu2+对花色苷有破坏作用，不耐氧化剂和还原剂，麦芽糖对其稳定性也有较大影响。花色苷的主要成分为飞燕草素-3,5-二葡萄糖苷和矢车菊素-3-半乳糖苷。
- Abstract:
- The red leaves of E.maackii in autumn and winter were used as materials to study the extraction process of anthocyanins in leaves and the stability under different conditions,and the components were identified by high performance liquid chromatography.The results showed that the best extraction method of anthocyanins in E.maackii leaves was 90% methanol-0.1 mol?L-1 hydrochloric acid as extraction solution,60 ℃ extraction temperature and extraction time three hours,solid-liquid ratio was 1∶40 g?mL-1.Anthocyanins were sensitive to sunlight,stable under acidic conditions,stable below 60 ℃.Pb2+、Fe2+、Fe3+ and Cu2+ had a destructive effect on anthocyanins.Anthocyanins were not resistant to oxidants and reducing agents.Maltose also had a great influence on their stability.The main components of anthocyanins were delphinidin-3,5-diglucoside and cyanidin-3-galactoside.
参考文献/References:
[1]JARKKO H,MATTILA P,KARJALAINEN R.Stability of anthocyanins in berry juices stored at different temperatures[J].Journal of Food Composition & Analysis,2013,31(1):12-19.[2]何静仁,邝敏杰,邓莉,等.膳食花色苷衍生物家族的结构及其形成机制[J].中国农业科学,2013,46(2):343-355.[3]中国植物志编委会.中国植物志（45卷）[M].北京：科学出版社，2004.[4]李淑梅.丝棉木在园林绿化中的应用[J].现代园艺,2015(10):114.[5]何顺志,张小勇,徐文芬,等.贵州卫矛属药用植物资源种类与地理分布的研究[J].中国野生植物资源,2013,32(5):41-44.[6]卢芳,周虹.丝棉木种子休眠特性及其解除[J].江苏农业科学，2018,46（24）：116-119．[7]宋红,李万义,丁格根其尔,等.不同贮藏方法对桃叶卫矛种子萌发和营养物质的影响[J].东北林业大学学报,2015,43(6):30-33,63.[8]田晓璇,李享,宋红.桃叶卫矛种子休眠机制及催芽方法初探[J].种子,2018,37(1):18-21,27.[9]孙焕顷,梁魁景,裴素检,等.提高丝棉木芽接卫矛成活率的关键技术[J].现代农村科技,2017(9):57-58.[10]余杰,郭慧敏,陈美珍.河东乌麦色素提取及其理化性质的研究[J].食品与发酵工业,2002(11):12-16.[11]邝敏杰,齐敏玉,何静仁,等.紫菜薹花色苷组分鉴定及其稳定性和抗氧化性[J].中国农业科学,2014,47(20):4067-4077.[12]罗赟,陈宗玲,宋卫堂,等.草莓果实花色苷成分组成鉴定及分析[J].中国农业大学学报,2014,19(5):86-94.[13]刘晓东,于晶.紫叶风箱果叶片花色素苷的提取及其稳定性[J].东北林业大学学报,2011,39(2):38-39,81.[14]岳桦,于淼.卫矛叶片花色苷的提取及稳定性研究[J].河南农业科学,2014,43(4):110-113.[15]苑玉莉,常雅宁,俞建瑛,等.紫苏中花色素的提取及储藏稳定性[J].华东理工大学学报(自然科学版),2013,39(2):156-160.[16]吴春太,周珺,姚行成,等.橡胶树叶片花色素苷的提取与稳定性及其品种间含量变化研究[J].中南林业科技大学学报,2017,37(12):15-20,45.[17]孙建霞,张燕,胡小松,等.花色苷的结构稳定性与降解机制研究进展[J].中国农业科学,2009,42(3):996-1008.[18]李云,赵昶灵,杨晓娜,等.花色苷分子结构与其稳定性以及呈色关系的研究进展[J].云南农业大学学报(自然科学版),2010,25(5):712-720.[19]ROMERO-DIEZ R,MATOS M,RODRIGUES L,et al.Microwave and ultrasound pretreatments to enhance anthocyanins extraction from different winelees[J].Food Chemistry,2019,272:258-266.[20]安小琦,王月华,孟宪军.‘蓓蕾’蓝靛果中花色苷组成鉴定及抗氧化能力比较分析[J].食品科学,2016,37(19):82-87.[21]胡彬,丁德辉,傅秀敏,等.钟冠报春苣苔花发育进程中花色素苷含量的变化[J].园艺学报,2018,45(1):117-125.
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备注/Memo
第一作者简介：宋鹏（1995-），男，硕士研究生，研究方向为园林植物生理及应用。E-mail:280882216@qq.com.责任作者：丁彦芬（1966-），女，硕士，副教授，硕士生导师，研究方向为园林植物生理、栽培及应用。E-mail:1281569022@qq.com.基金项目：国家林业局“948”资助项目（2013-4-30）；江苏高校品牌专业建设工程资助项目（PPZY2015A063）。收稿日期：2019-04-28