[1]王葆生,廉勇,张艳萍,等.胡萝卜游离小孢子胚状体诱导技术优化[J].北方园艺,2019,43(13):68-72.[doi:10.11937/bfyy.20185051]
　WANG Baosheng,LIAN Yong,ZHANG Yanping,et al.Optimization of Embryo Induction System for Isolated Microspore Culture in Carrot[J].Northern Horticulture,2019,43(13):68-72.[doi:10.11937/bfyy.20185051]
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胡萝卜游离小孢子胚状体诱导技术优化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年13 页码: 68-72 栏目: 出版日期: 2019-07-15
Title:
Optimization of Embryo Induction System for Isolated Microspore Culture in Carrot
作者:
王葆生; 廉勇; 张艳萍; 刘湘萍; 扈顺; 陈源闽
（内蒙古自治区农牧业科学院 蔬菜研究所，内蒙古 呼和浩特 010031）
Author(s):
WANG Baosheng; LIAN Yong; ZHANG Yanping; LIU Xiangping; HU Shun; CHEN Yuanmin
(Institute of Vegetable,Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences,Hohhot,Inner Mongolia 010031)
关键词:
胡萝卜; 小孢子; 胚状体; 优化
Keywords:
carrot; microspore; embryoid; optimization
DOI:
10.11937/bfyy.20185051
文献标志码:
A
摘要:
以10种基因型胡萝卜品种为试材，采用游离小孢子离体培养的方法，对影响胡萝卜小孢子诱导的因素进行了研究，以期为胡萝卜小孢子培养过程中的相关环节提供参考。结果表明：有3个基因型诱导出胚状体，不同基因型之间胚诱导率差异明显，表明基因型是影响小孢子胚诱导的重要因素；盛花期取材进行诱导的出胚率显著高于其它2个时期，表明盛花期为最佳取材时期；33 ℃处理2 d的出胚率最高，为胡萝卜小孢子胚诱导的最佳热激条件；激素最佳配比为0.5 mg?L-1 2,4-D；培养基中添加一定量的活性炭有利于小孢子诱导产生胚状体，最佳浓度为0.2 g?L-1。
Abstract:
Ten genotypes of carrot were used to study the effect factors of embryo induction.The results showed that three of the ten genotypes induced embryoids,and the rate of embryogenesis varied significantly among different genotypes,indicating that genotype was an important factor affecting microspore embryo induction.The rate of embryo induction at full flowering stage was significantly higher than that at the other two stages,indicating that full flowering stage was the best time for embryo induction.Among all the experimental conditions,heat shock at 33 ℃ for 2 days had the highest embryogenesis rate,which was the best heat shock condition for microspore embryo induction of carrot.The best ratio of hormone was 0.5 mg?L-1 2,4-D added in the medium.Adding a certain amount of activated carbon to the medium was beneficial to the embryo induction,and the optimum concentration was 0.2 g?L-1.

参考文献/References:

[1]李靖,李焕秀,李敏,等.蔬菜花药培养研究进展[J].中国蔬菜,2005,1(6):36-38.[2]王葆生,刘湘萍,廉勇,等.单倍体育种技术研究进展[J].北方农业学报,2018（5）:44-49.[3]DWIVEDI S L,BRITT A B,TRIPATHI L,et al.Haploids:Constraints and opportunities in plant breeding[J].Biotechnology Advances,2015,33(6):812-829.[4]LICHTER R.Induction of haploid plants from isolated pollen of Brassica napus[J].Zeitschrift Für Pflanzenphysiologie,1982,105(5):427-434.[5]ANDERSEN S B,CHRISTIANSEN I,FARESTVEIT B.Carrot (Daucus carota,L.):In vitro production of haploids and field trials[M]//Haploids in Crop Improvement I.Springer Berlin Heidelberg,1990:393-402.[6]MATSUBARA S,DOHYA N,MURAKAMI K.Callus formation and regeneration of adventitious embryos from carrot,fennel and mitsuba microspores by anther and isolated microspore cultures[J].Acta Horticulturae,1995，392:129-138.[7]GRECKA K,KOWALSKA U,KRZYZANOWSKA D,et al.Obtaining carrot (Daucus carota L.) plants in isolated microspore cultures[J].Journal of Applied Genetics,2010,51(2):141-147.[8]李金荣,欧承刚,庄飞云,等.胡萝卜游离小孢子培养及其发育过程研究[J].园艺学报,2011,38(8):1539-1546.[9]FERRIE A M R,MLLERS C.Haploids and doubled haploids in Brassica spp.for genetic and genomic research[J].Plant Cell Tissue & Organ Culture,2011,104(3):375-386.[10]GRECKA K,KRZYZANOWSKA D,GRECKI R.The influence of several factors on the efficiency of androgenesis in carrot[J].Journal of Applied Genetics,2005,46(3):265-269.[11]唐兵,陶莲,卢松,等.白菜游离小孢子培养高频胚诱导技术体系优化[J].热带作物学报,2017(10):1913-1920.[12]米哲,李云昌,梅德圣,等.甘蓝型油菜小孢子培养影响因素研究及再生苗早期倍性鉴定[J].华北农学报,2011,26(5):97-102.[13]苏贺楠,韩风庆,杨丽梅,等.结球甘蓝小孢子培养条件优化及高代自交系胚状体诱导研究[J].中国蔬菜,2018(4):30-36.[14]盛鹏,张秀荣,岳艳玲,等.光照和激素对耐热型大白菜游离小孢子培养的影响[J].吉林农业大学学报,2011,33(6):649-653.[15]姜凤英,冯辉,王超楠.小白菜小孢子培养胚状体的诱导[J].沈阳农业大学学报,2006,37(5):763-765.[16]郭世星,牛应泽,余学杰,等.活性炭对甘蓝型油菜(Brassica napus L.)小孢子胚胎发生的影响[J].种子,2005(7):37-39.[17]张丽,郑鹏婧.春白萝卜游离小孢子培养的研究[J].北方园艺,2013(23):31-33.

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备注/Memo

第一作者简介：王葆生（1983-），男，内蒙古包头人，博士，副研究员，现主要从事蔬菜新品种培育及生物技术辅助育种等研究工作。E-mail:cauwbs@126.com.基金项目：内蒙古自治区自然科学基金博士基金资助项目（2014BS0314）；蒙古农牧业创新基金资助项目（2017CXJJN17-2）；内蒙古自治区科技计划资助项目（201702083，201702085）。收稿日期：2019-01-23

更新日期/Last Update: 2019-08-01