[1]张业尼,钱磊,刘逸寒,等.光照对灵芝液体发酵胞外多糖合成的影响[J].北方园艺,2019,43(02):154-159.[doi:10.11937/bfyy.20181731]
　ZHANG Yeni,QIAN Lei,LIU Yihan,et al.Effect of Light on Exopolysaccharide Synthesis in Liquid Fermentation of Ganoderma lucidum[J].Northern Horticulture,2019,43(02):154-159.[doi:10.11937/bfyy.20181731]
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光照对灵芝液体发酵胞外多糖合成的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年02 页码: 154-159 栏目: 出版日期: 2019-01-15
Title:
Effect of Light on Exopolysaccharide Synthesis in Liquid Fermentation of Ganoderma lucidum
作者:
张业尼1; 钱磊2; 3; 刘逸寒4; 5; 孔庆学1; 张志军2; 3
（1.天津农学院 食品科学与生物工程学院，天津 300384；2.天津市林业果树研究所，天津 300384；3.农业部农产品贮藏保鲜重点实验室，天津 300384；4.天津科技大学 生物工程学院，天津 300457；5.工业发酵微生物教育部重点实验室，天津 300457）
Author(s):
ZHANG Yeni1; QIAN Lei2; 3; LIU Yihan4; 5; KONG Qingxue1; ZHANG Zhijun2; 3
(1.College of Food Science and Biotechnology,Tianjin Agricultural University,Tianjin 300384;2.Tianjin Research Institute of Forestry and Pomology,Tianjin 300384;3.Key Laboratory of Storage of Agro-products,Ministry of Agriculture,Tianjin 300384;4.College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457;5.Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin 300457)
关键词:
灵芝; 光照; 生物量; 胞外多糖; 蓝光
Keywords:
Ganoderma lucidum; illumination; biomass; exopolysaccharide; blue light
DOI:
10.11937/bfyy.20181731
文献标志码:
A
摘要:
以灵芝为试验材料，采用干质量法测定生物量，苯酚-硫酸法测定胞外多糖产量，研究了单色LED光照处理对灵芝菌丝体液体发酵合成胞外多糖的影响。结果表明：在80 μmol·m-2·s-1 LED光照12 h条件下，不同LED光照处理均能促进细胞生长和胞外多糖合成，其中白光LED最有利于细胞生长，蓝光LED最有利于胞外多糖合成。综合考虑生物量和胞外多糖产量，以100 μmol·m-2·s-1蓝光LED光照10 h效果最佳，生物量和胞外多糖产量分别为15.16 g·L-1和3.102 g·L-1，比黑暗处理提高了45.91%和109.74%。光照对灵芝细胞生长和胞外多糖合成具有调控作用，为灵芝液体发酵合成胞外多糖的高效定向生产提供指导。
Abstract:
The effects of different LED light treatment on exopolysaccharide synthesis in the liquid fermentation of Ganoderma lucidum were studied. The biomass and the yield of exopolysaccharide were determined by dry weight method and phenol-sulfuric acid method,respectively.The results showed that under the condition of light treatment for 12 hours with intensity of 80 μmol·m-2·s-1,different LED light treatments could promote cell growth and exopolysaccharide synthesis.White LED light was most conducive to cell growth and blue LED light was best for the synthesis of exopolysaccharides.Taking into account the biomass and the yield of exopolysaccharide,blue LED light treatment for 10 hours with intensity of 100 μmol·m-2·s-1 had the best effect,the biomass and the yield of exopolysaccharide was 15.16 g·L-1 and 3.102 g·L-1,respectively,which was 45.91% and 109.74% higher than those of dark treatment.Light has a regulatory effect on cell growth and exopolysaccharide synthesis,which provided theoretical guidance for efficient production of Ganoderma lucidum exopolysaccharide.

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备注/Memo

第一作者简介：张业尼（1984-），女，博士，讲师，研究方向为食品科学与生物工程。E-mail:yenizhang@hotmail.com.责任作者：张志军（1969-），男，本科，研究员，研究方向为食用菌遗传育种及栽培与加工。E-mail:tjzhangzj@sina.cn.基金项目：天津市科技计划资助项目（17ZXBFNC00160）；天津市蔬菜现代农业产业技术体系创新团队资助项目（ITTVRS2017008）；天津市“131”创新型人才团队资助项目（20180337）。收稿日期：2018-06-27

更新日期/Last Update: 2019-02-21