LUAN Feishi,LYU Huiling,ZHU Zicheng,et al.Identification and Characterization of CIPK Family Genes in Watermelon[J].Northern Horticulture,2018,42(08):1-7.[doi:10.11937/bfyy.20174641]
西瓜CIPK家族基因的鉴定与特征分析
- Title:
- Identification and Characterization of CIPK Family Genes in Watermelon
- 文献标志码:
- A
- 摘要:
- CIPK（Calcineurin B-like-interacting protein kinase）是一类植物中十分重要的蛋白激酶，主要参与植物的生长发育和抗逆过程。为了揭示CIPK基因家族在西瓜生长发育与抗逆防御机制中的重要作用，该研究基于西瓜基因组数据库，利用生物信息学手段，鉴定并分析了西瓜CIPK家族基因的基因结构、染色体定位、系统发育和氨基酸理化性质等特征。结果表明：西瓜中有19个CIPK家族成员，分别分布于1～6、8，10号的染色体上，编码氨基酸序列长度为330～565 bp，其中18个ClCIPK氨基酸序列含NAF功能域，仅有1个CIPK（ClCIPK9）基因不含有此结构；CIPK基因家族成员的基因结构大致分为内含子富集和内含子缺失2类；进化树聚类分析结果将所有的西瓜及拟南芥CIPK蛋白分为5类，在每一类中都包含西瓜和拟南芥的CIPK家族成员，说明它们具有很高的同源性，并推测它们可能参与了西瓜的多种生物学过程。该研究结果以期为ClCIPK基因在西瓜育种中的功能研究与应用提供重要的理论基础。
- Abstract:
- Calcineurin B-like-interacting protein kinase (CIPK) is one kind of kinases,which are very important for plant in responding environmental stress and the progress of development.To reveal the vital role of CIPK gene family in watermelon growth,development and anti-defense mechanism,in our study,based on watermelon genome database,the CIPK family genes structure,positions on chromosome,phylogenetic relationship,physical and chemical properties were analyzed by bioinformatics tools.The results showed that 19 kinds of ClCIPK genes were identified from watermelon whole genome,they were unevenly distributed in the chromosomes No1-6,8,10,respectively.The putative encoded proteins length of ClCIPK ranged from 330 to 565 bp.Based on gene structures,NAF domains was found in 18 of ClCIPK family genes but not in ClCIPK9.All ClCIPK genes were divided into exon-rich and exon-poor two groups;and phylogenetic analysis revealed that all the ClCIPK proteins were divided into five major clades,and every clades contained a part of CIPK genes from watermelon and arabidopsis,which indicated that the ClCIPK family had a high homology relation with AtCIPK.Based on these results,we speculated that ClCIPK genes might involve in multiple biological processes,and our research would provide a theoretical basis for the functional dissection and utilization of CIPK genes in watermelon breeding.
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备注/Memo
第一作者简介：栾非时（1964-），女，博士，教授，博士生导师，研究方向为西甜瓜分子遗传育种。E-mail：luanfeishi@neau.edu.cn.基金项目：国家自然科学基金面上资助项目（31572144）；国家西甜瓜产业技术体系-分子育种岗位资助项目（CARS-25）。收稿日期：2018-02-23