[1]李会端,江岸,余建中.生姜总黄酮甲醇浸提工艺的响应面优化及提取液对羟自由基清除活性[J].北方园艺,2017,41(23):155-164.[doi:10.11937/bfyy.20172383]
　LI Huiduan,JIANG An,YU Jianzhong.Optimization of Extract Methodology of Total Flavonoids From Ginger and Hydroxyl Radicals Scavenging Effect[J].Northern Horticulture,2017,41(23):155-164.[doi:10.11937/bfyy.20172383]
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生姜总黄酮甲醇浸提工艺的响应面优化及提取液对羟自由基清除活性

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年23 页码: 155-164 栏目: 出版日期: 2017-12-10
Title:
Optimization of Extract Methodology of Total Flavonoids From Ginger and Hydroxyl Radicals Scavenging Effect
作者:
李会端; 江岸; 余建中
楚雄师范学院 化学与生命科学学院，云南 楚雄 675000
Author(s):
LI Huiduan; JIANG An; YU Jianzhong
Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong,Yunnan 675000
关键词:
响应面分析; 生姜; 总黄酮; 甲醇浸提; 得率; 羟自由基清除活性
Keywords:
response surface methodology; ginger; total flavonoids; methanol soaked; extraction ratio; hydroxyl radicals scavenging effect
DOI:
10.11937/bfyy.20172383
文献标志码:
A
摘要:
以生姜为试材，甲醇水溶液为溶剂，采用浸提法提取总黄酮，通过设计单因素试验和Box-benhnken design响应面优化生姜总黄酮的提取工艺，大孔树脂吸附纯化总黄酮提取液，设计体外抗氧化试验，探究了甲醇浸提生姜总黄酮工艺的响应面优化过程及提取物对羟自由基的清除活性，以期为生姜作为调味品的营养和药用价值开发应用提供前期研究基础。结果表明：甲醇浸提生姜总黄酮的最佳工艺条件为甲醇体积分数60%、料液比3∶30 g?mL-1、浸提温度60 ℃和浸提时间3.0 h，在此条件下，试验测得总黄酮得率最大值为0.497%。与多元响应模型理论预测值0.538%吻合良好，佐证了响应面分析是一种多变量复杂工艺过程优化的有效方法，获得的多元回归模型对目标响应值具有精准的预测作用。体外抗氧化活性对照试验结果显示，相同浓度的3种抗氧化剂对?OH清除活性依次为抗坏血酸>BHT>生姜总黄酮，3种抗氧化剂的浓度和清除率有显著的量-效关系。
Abstract:
The total flavonoids were extracted from ginger by using methanol as solvent.The extraction process was optimized by single factor test and Box-benhnken design,the total flavonoids were purified by macroporous resin.In vitro antioxidant test was also designed.In order to provide the preliminary research basis for the development and application of nutrition and medicinal value of the ginger as condiment,the optimization process of the total flavonoids extraction by response surface methodology and the scavenging effect on hydroxyl radical were studied.The results indicated that the optimum extract conditions were methanol concentration of 60%,solid-liquid ratio of 3∶30 g?mL-1,extraction temperature of 60 ℃ and time of 3.0 hours.The maximum experimental extraction ratio was 0.497% by RSM under the optimum extract conditions.The experimental extraction ratio matched well with the theoretical value of 0.538% by solving the multiple regression equation.RSM had been proved to be an effective technique for optimization of extraction process and the fitted quadratic model had a predictive effect on target extracts.The scavenging effect of ginger extracts,BHT and L-ascorbic acid on ?OH with the same concentration were sorted by L-ascorbic acid>BHT>ginger extract,and all the three antioxidant regents displayed a significant dose-effect relationship.

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备注/Memo

第一作者简介：李会端（1983-），女，博士，副教授，现主要从事天然产物有效成分的化学提取工艺等的教学与科研等工作。E-mail：lhd08@cxtc.edu.cn.

基金项目：大学生创新创业训练资助项目（16449）；国家自然科学基金资助项目（21643006）；国家公派访问学者资助项目（201608530002）。
收稿日期：2017-07-18

更新日期/Last Update: 2017-12-20