JIANG Shao-juan,LIU Xiao-li.Study on Ultrasonic Extraction of Flavonoids From Ginger and Antibacterial Activity[J].Northern Horticulture,2014,38(04):120-123.
生姜黄酮超声提取及其抑菌活性研究
- Title:
- Study on Ultrasonic Extraction of Flavonoids From Ginger and Antibacterial Activity
- 文章编号:
- 1001-0009(2014)04-0120-04
- 分类号:
- S 632.5
- 文献标志码:
- A
- 摘要:
- 以生姜为原料，采用超声波辅助提取技术，通过单因素试验和正交实验设计优化了黄酮的提取工艺条件，同时采用滤纸片法进行了生姜黄酮抑菌活性的研究。结果表明：生姜黄酮的超声提取最佳工艺条件为80%的乙醇，料液比1∶12 g/mL，在50℃下提取15 min；生姜黄酮对4种菌均有不同程度的抑制作用，且抑制作用的强弱次序为枯草芽孢杆菌>黑曲霉>青霉菌>大肠杆菌。
- Abstract:
- With ginger as raw material，the ultrasonic extraction of flavonoids from ginger was studied.The extraction process of flavonoids was optimized with single factor experiment and orthogonal experiment，and the antibacterial activity of flavonoids from ginger was studied by filter paper method.The results showed that the optimum extraction conditions were determined as follows：alcohol concentration 80%，solid-liquid ratio 1∶12 g/mL，extraction temperature 50℃，extraction time 15 minutes.And ginger flavonoids showed different antibacterial activity for the four bacteria，the order of the strength of the inhibitory effect was Bacillus subitlis>Aspergillus niger>Penicillium>Escherichia coli.
参考文献/References:
[1]郭雅翠.溶剂热法提取生姜总黄酮的工艺研究[J].安徽农业科学,2010,38(34):19371-19373. [2]莫开菊,柳圣,程超.生姜黄酮的抗氧化活性研究[J].食品科学,2006,27(9):110-115. [3]杨洋.生姜黄酮的提取及其抗氧化活性的研究[J].食品科学,2002,23(4):45-50. [4]王啸.生姜活性部位与成分研究进展[J].中医研究,2009,22(12):53-55. [5]朱茂田,马力.生姜黄酮的分离和纯化工艺研究[J].食品研究与开发,2006,27(3):57-58. [6]辛莉,黄桂东,钟先锋,等.生姜总黄酮提取工艺研究[J].中药材,2008,31(5):766-768. [7]杨郭,颜杰,杨虎,等.应用二次回归正交设计优化生姜黄酮的提取工艺[J].云南化工,2009,36(4):24-27. [8]程丽娟,薛泉宏.微生物学实验技术[M].西安:世界图书出版公司,2000:108. [9]王瑞君.几种中草药对金黄色葡萄球菌体外抑制作用的研究[J].现代食品科技,2009,25(9):1104-1106.
相似文献/References:
[1]杜来顺,冯建军,李晓华,等.生姜玻璃化法超低温保存技术[J].北方园艺,2014,38(13):123.
DU Lai-shun,FENG Jian-jun,LI Xiao-hua,et al.Ginger Vitrification Cryopreservation Technology[J].Northern Horticulture,2014,38(04):123.
[2]王媛媛,赵炳坤,郭衍银,等.溶剂提取生姜油的工艺优化研究[J].北方园艺,2014,38(11):130.
WANG Yuan-yuan,ZHAO Bing-kun,GUO Yan-yin,et al.Study on Optimization Process of Ginger Oil by Solvent Extraction[J].Northern Horticulture,2014,38(04):130.
[3]武晓林,陈建强,李 敏,等.不同部位长白楤木皂苷物质含量及其工艺研究[J].北方园艺,2014,38(11):150.
WU Xiao-lin,CHEN Jian-qiang,LI Min,et al.Study on the Content of Different Parts and the Technology of Aralia continentalis Kitag.[J].Northern Horticulture,2014,38(04):150.
[4]韩春梅,李春龙,叶少平,等.不同栽培模式下生姜品质及经济效益的比较研究[J].北方园艺,2013,37(22):41.
HAN Chun-mei,LI Chun-long,YE Shao-ping,et al.Comparative Study on the Quality and Economic Benefit of Ginger Under Different Cultivated Patterns[J].Northern Horticulture,2013,37(04):41.
[5]韩春梅,李春龙,叶少平,等.生姜水浸液对生姜幼苗形态和光合指标的影响 [J].北方园艺,2013,37(17):1.
HAN Chun-mei,LI Chun-long,YE Shao-ping,et al.Effects of Ginger Aqueous Extracts on Morphology and Photosynthesis Indexes of Ginger Seedling[J].Northern Horticulture,2013,37(04):1.
[6]姜少娟.毛竹叶多糖超声提取工艺研究[J].北方园艺,2013,37(13):166.
JIANG Shao-juan.Research on Ultrasonic Wave Extraction of Polysaccharides from Phyllostachys pubescens Leaves[J].Northern Horticulture,2013,37(04):166.
[7]王丽霞,刘坤.超声波辅助提取玉米须黄酮工艺[J].北方园艺,2014,38(06):137.
WANG Li-xia,LIU Kun.Extraction Technology on Flavonoids From Corn Silk by Ultrasonic-assisted[J].Northern Horticulture,2014,38(04):137.
[8]杨桂珍,陈伟珍,冼彩霞,等.超声提取鼠曲草中水溶性黄酮类化合物和多糖工艺研究[J].北方园艺,2014,38(07):148.
YANG Gui-zhen,CHEN Wei-zhen,XIAN Cai-xia,et al.Study on Ultrasonic Extraction of Water-soluble Flavonoids and Polysaccharides From Gnaphalium affine[J].Northern Horticulture,2014,38(04):148.
[9]苏艳玲,巫东堂,刘夏薇.大蒜和生姜复配液对蔬菜保鲜效果的影响[J].北方园艺,2013,37(11):122.
SU Yan-ling,WU Dong-tang,LIU Xia-wei.Research on the Fresh Effect of Vegetables Using the Compound Liquid of Galic and Ginger[J].Northern Horticulture,2013,37(04):122.
[10]韩春梅,李春龙,叶少平,等.不同栽培模式对生姜形态指标和产量的影响[J].北方园艺,2013,37(12):18.
HAN Chun-mei,LI Chun-long,YE Shao-ping,et al.Effects of Different Cultivation Patterns on Morphological Index and Rhizome Yields of Ginger[J].Northern Horticulture,2013,37(04):18.
备注/Memo
第一作者简介：姜少娟（1979-），女，陕西西安人，硕士，讲师，现主要从事天然产物化学等研究工作。