[1]刘晓梦,孟想想,张威威,等.洋甘菊的组织培养技术[J].北方园艺,2018,42(02):72-76.[doi:10.11937/bfyy.20171223]
　LIU Xiaomeng,MENG Xiangxiang,ZHANG Weiwei,et al.Tissue Culture Technique for Matricaria chamomilia[J].Northern Horticulture,2018,42(02):72-76.[doi:10.11937/bfyy.20171223]
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洋甘菊的组织培养技术

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年02 页码: 72-76 栏目: 出版日期: 2018-01-25
Title:
Tissue Culture Technique for Matricaria chamomilia
作者:
刘晓梦1; 孟想想1; 张威威1; 廖咏玲1; 常杰2; 3; 许锋1
1.长江大学 园艺园林学院，湖北 荆州 434025；2.靶向抗肿瘤药物湖北省协同创新中心，湖北 荆门 448000；3.荆楚理工学院 化工与药学院，湖北 荆门 448000
Author(s):
LIU Xiaomeng1; MENG Xiangxiang1; ZHANG Weiwei1; LIAO Yongling1; CHANG Jie2; 3; XU Feng1
1.College of Horticulture and Landscape Architecture,Yangtze University,Jingzhou,Hubei 434025;2.Hubei Collaborative Innovation Center of Targeted Antitumor Drug,Jingmen,Hubei 448000;3.College of Chemical Engineering and Pharmacy,Jingchu University of Technology,Jingmen,Hubei 448000
关键词:
洋甘菊; 组织培养; 诱导率; 激素; 消毒措施
Keywords:
Matricaria chamomilla; tissue culture; induction rate; hormone; disinfection measures
DOI:
10.11937/bfyy.20171223
文献标志码:
A
摘要:
以洋甘菊（Matricaria chamomilla L.）的嫩茎和幼叶为外植体，采用单因子试验方法，研究不同消毒措施、不同激素浓度配比对洋甘菊愈伤组织诱导的影响，以期为建立洋甘菊再生体系以及通过基因工程手段改良洋甘菊提供参考依据。结果表明：洋甘菊幼嫩的茎段为组织培养的最佳外植体材料，愈伤组织的诱导率高达90%；以幼嫩茎段为外植体，最佳灭菌措施为新鲜的嫩茎用无菌水冲洗30 min，75%的酒精浸泡10 s，3 5%的次氯酸钠浸泡10 min，诱导率高达86 7%，污染率低至13 3%；其愈伤组织诱导的最佳培养基为MS+6-BA 1 5 mg?L-1+NAA 1 2 mg?L-1。
Abstract:
Young stems and young leaves of Matricaria chamomilia were used as explants,by adopting the method of single factor experiment,the influence of different disinfection measures and different hormone concentrations on callus induction of M.chamomilla was studied,in order to establish M.chamomilla regeneration system and genetic engineering methods.The results showed that young stems were the best explants for tissue culture with a high level of callus forming rate of 90%.The best condition of sterilization with young stems was as follow:fresh young stems wash under sterile water for 30 minutes,followed with 75% alcohol for 10 seconds and then 3 5% sodium hypochlorite for 10 minutes,the induction rate was as high as 86 7%,pollution rate was as low as 13 3%;the best medium for callus induction was MS + 6-BA 1 5 mg?L-1 + NAA 1 2 mg?L-1.

参考文献/References:

[1]景玉霞，郭玉婷，兰卫.洋甘菊中6种微量元素含量的测定[J].新疆医科大学学报，2013（9):1282-1283.

[2]SU S,LIU X,PAN G,et al.In vitro characterization of a （E)-β-farnesene synthase from Matricaria recutita L.and its up-regulation by methyl jasmonate[J].Gene,2015,571（1):58-64.
[3]杨俊杰，袁艺，王小娟，等.洋甘菊组织培养及植株再生[J].激光生物学报，2009，18（6):825-829.
[4]ROCHA N F,RIOS E R,CARVALHO A M,et al.Anti-nociceptive and anti-inflammatory activities of （-)-α-bisabolol in rodents[J].Naunyn-Schmiedeberg′s Archives of Pharmacology,2011,384（6):525-533.
[5]SON Y J,KWON M,RO D K,et al.Enantioselective microbial synthesis of the indigenous natural product （-)-α-bisabolol by a sesquiterpene synthase from chamomile （Matricaria recutita)[J].Biochemical Journal,2014,463（2):239-248.
[6]SHARKEY T D,GRAY D W,PELL H K,et al.Isoprene synthase genes form a monophyletic clade of acyclic terpene synthases in the tps-b terpene synthase family[J].Evolution,2013,67（4):1026-1040.
[7]黄致喜，王慧辰.重要单环倍半萜香料:甜没药烯和甜没药醇合成的进展[J].香料香精化妆品，1993（3):9-13.
[8]KIM S,JUNG E,KIM J H,et al.Inhibitory effects of （-)-α-bisabolol on LPS-induced inflammatory response in RAW264 7 macrophages [J].Food & Chemical Toxicology an International Journal Published for the British Industrial Biological Research Association,2011,49（10):2580-2585.
[9]BOHLMANN J,KEELING C I.Terpenoid biomaterials [J].Plant Journal for Cell & Molecular Biology,2008,54（4):656-669.
[10]PERALTAYAHYA P P,OUELLET M,CHAN R,et al.Identification and microbial production of a terpene-based advanced biofuel[J].Nature Communications,2011,2（9):2749-2763.
[11]FARHOUD R.Chemical constituents and antioxidant properties of Matricaria recutita and Chamaemelum nobile essential oil growing wild in the south west of Iran[J].Journal of Essential Oil Bearing Plants,2013,16（4):531-537.
[12]SILVRIO M S,DELVECHIOVIEIRA G,PINTO M A,et al.Chemical composition and biological activities of essential oils of Eremanthus erythropappus （DC) McLeisch （Asteraceae)[J].Molecules,2013,18（8):9785-9796.
[13]SALLAUD C,RONTEIN D,ONILLON S,et al.A novel pathway for sesquiterpene biosynthesis from Z,Z-farnesyl pyrophosphate in the wild tomato solanum habrochaites[J].Plant Cell,2009,21（1):301-317.
[14]YU F,UTSUMI R.Diversity,regulation,and genetic manipulation of plant mono-and sesquiterpenoid biosynthesis[J].Cellular & Molecular Life Sciences,2009,66（18):3043-3052.
[15]FLGGE U I,GAO W.Transport of isoprenoid intermediates across chloroplast envelope membranes[J].Plant Biology,2004,7（1):91-97.
[16]LICHTENTHALER H K,SCHWENDER J,DISCH A,et al.Biosynthesis of isoprenoids in higher plant chloroplasts proceeds via a mevalonate-independent pathway[J].Febs Letters,1997,400（3):271-274.
[17]WANG H,HAN J,KANAGARAJAN S,et al.Studies on the expression of sesquiterpene synthases using promoter-β-glucuronidase fusions in transgenic Artemisia annua L.[J].PLoS One,2013,8（11):e80643-e80643.
[18]JAKOVLEV V,ISAAC O,THIEMER K,et al.Pharmacological investigations with compounds of chamomile ii.new investigations on the antiphlogistic effects of （-)-alpha-bisabolol and bisabolol oxides[J].Planta Medica,1979,35（2):125-140.
[19]夏秋香,白红彤,孙凌臣,等.药用植物母菊的成分和功能研究进展[J].园艺学报,2012,39（9):1859-1864.
[20]沙红，廖康.药用植物洋甘菊的组织培养研究[J].新疆农业大学学报，2004，27（4):16-18.
[21]SRIVASTAVA J K,SHANKAR E,GUPTA S.Chamomile:A herbal medicine of the past with bright future[J].Molecular Medicine Reports,2010,3（6):895-901.
[22]SHARAFZADEH S,ALIZADEH O.German and Roman chamomile[J].Journal of Applied Pharmaceutical Science,2011,1（10):1-5.

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备注/Memo

第一作者简介：刘晓梦（1992-），女，硕士研究生，研究方向为植物分子生物学。E-mail:liuxm925@163.com.

责任作者：许锋（1979-），男，博士，教授，现主要从事药用植物次生代谢等研究工作。E-mail:xufeng198@126.com.
基金项目：国家自然科学基金资助项目（31400603）；大学生创新创业训练计划资助项目（104892014043）；荆州市科技发展计划资助项目（2015Z21015）。
收稿日期：2017-07-18

更新日期/Last Update: 2018-01-26