GUO Fukuan,WANG Shurong,MENG Junlong,et al.Screening of Optimal Culture Conditions of Three Strains of Macrofungi[J].Northern Horticulture,2017,41(13):161-164.[doi:10.11937/bfyy.20170038]
三株大型真菌最佳培养条件的筛选
- Title:
- Screening of Optimal Culture Conditions of Three Strains of Macrofungi
- Keywords:
- macrofungi; mycelium; culture conditions
- 文献标志码:
- A
- 摘要:
- 以采自庞泉沟国家自然保护区的3种大型真菌（野蘑菇、罗曼尼蘑菇、梨形马勃）为试材，对子实体进行组织分离获得菌株，采用观察、测量、统计、分析等方法，研究比较了5种不同的碳源和氮源对3种菌株的菌丝生长速度的影响。结果表明：野蘑菇和罗曼尼蘑菇菌丝生长的最适碳源均为玉米粉，梨形马勃菌丝生长的最适碳源为葡萄糖；罗曼尼蘑菇和梨形马勃菌丝生长的最适氮源为硫酸铵，野蘑菇菌丝生长的最适氮源为硝酸铵。该研究为食用菌资源的开发利用奠定了基础。
- Abstract:
- Three species of macrofungi (Agaricus arvensis,Agaricus romagnesii,Lycoperdon pyriforme) from Pangquangou National Nature Reserve were used as the test materials,the strains from subsidiary entities was gotten by isolating tissue.The influence of five different carbon source and nitrogen source on the three strains of mycelium growth rate were researched by adopting such methods as observation,survey,statistics,analysis and so on.The results showed that the optimal carbon source of Agaricus arvensis and Agaricus romagnesii mycelium′s growth was corn flour,the optimal carbon source of Lycoperdon pyriforme mycelium′s growth was glucose;the optimal nitrogen source of Agaricus romagnesii and Lycoperdon pyriforme mycelium′s growth was ammonium sulfate;the optimum nitrogen source of Agaricus arvensis mycelium′s growth was ammonium nitrate.The research laid the foundation for edible fungus resources′ development and utilization.
参考文献/References:
[1]王瑞华.山东省菌物资源调查（Ⅰ）及部分虫生真菌的分子生物学研究[D].济南:山东中医药大学,2013. [2]吕彩莲.野蘑菇菌丝体培养条件的研究[D].呼和浩特:内蒙古农业大学,2011. [3]黄浩,肖正端,邓春英,等.广东石门台自然保护区大型真菌多样性研究(Ⅰ)[J].广东林业科技,2010(5):40-44. [4]阿历索保罗C J,布莱克韦尔M.菌物学概论[M].4版.姚一建,李玉,译.北京:中国农业出版社,2002. [5]杨勇,张凤英,陈岑.PDA培养基改良配方的研究[J].酿酒科技,2012(4):29-31. [6]徐冰洁.不同碳源条件下功能菌共代谢降解典型PPCPs的效能与机理[D].上海:东华大学,2014. [7]韩春华.杏鲍菇碳氮营养生理的研究[D].保定:河北农业大学,2003. [8]黄奕.食用菌栽培[M].3版.北京:高等教育出版社,2008. [9]赵亚东.不同培养料对秀珍菇生长发育、产量及胞外酶的影响[D].南京:南京农业大学,2011. [10]胡章喜,安民,段舜山,等.不同氮源对布朗葡萄藻生长、总脂和总烃含量的影响[J].生态学报,2009(6):3288-3294.
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备注/Memo
第一作者简介：郭富宽（1993-），男，硕士研究生，研究方向为食品科学。E-mail:18235446743@163.com.责任作者：常明昌（1964-），男，本科，教授，现主要从事食用菌等研究工作。E-mail:sxmushroom@126.com.基金项目：山西农业大学科研启动基金资助项目[JP4]（2014YJ18）；山西省煤基重点科技攻关资助项目（FT20140301）；2015 年度山西省高校协同创新中心资助项目。