XIE Chunqin,CAO Zheng,FAN Junmin,et al.Optimization of Wild Isaria cicadae Stain Liquid Culture[J].Northern Horticulture,2016,40(22):165-169.[doi:10.11937/bfyy.201622041]
野生蝉花液体菌种培养条件的优化
- Title:
- Optimization of Wild Isaria cicadae Stain Liquid Culture
- Keywords:
- Cordyceps cicadae; liquid strain; medium
- 文献标志码:
- A
- 摘要:
- 以野生蝉花菌种为试材，采用液体培养法，研究了碳源、氮源、微量营养和pH对野生蝉花菌种生长的影响，以期为标准化、规模化、产业化人工栽培蝉花提供优质种源。结果表明：将野生蝉花菌株接种到液体培养基中，在19 ℃，转速120 r?min-1恒温振荡摇床内培养，蝉花菌种的最适液体培养基组分为可溶性淀粉20 g，奶粉10 g，磷酸二氢钾0.5 g，硫酸镁1 g，维生素B12 10片，水1 000 mL，最适pH 5.0~6.0。
- Abstract:
- Isaria cicadae strain was used as material.The effect of different carbon sources，nitrogen sources，micronutrients，and pH on Isaria cicadae strain growth in liquid culture were studied.The results showed that when the culture condition was 19 ℃，revolving speed 120 r?min-1,the best component of I.cicadae strain were soluble starch 20 g，milk powder 10 g，KH2PO4 0.5 g，MgSO4 1 g and vitamin B12 10 plates，water 1 000 mL,initial pH 5.0-6.0.
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备注/Memo
第一作者简介：谢春芹（1976-)，女，硕士，副教授，现主要从事食药用真菌教学与科研等工作。E-mail：xiechunqin@163.com.责任作者：贾君（1966-)，女，博士，教授，现主要从事食品检测与分析等研究工作。E-mail：jnujia66@163.com.基金项目：2015年江苏省“六大人才高峰”资助项目（NY-024)；江苏农林职业技术学院资助项目（2014KJ28)。