CHENG Ming,LI Houhua,HE Zisen,et al.Tissue Culture of Endangered Plant Syringa pinnatifolia[J].Northern Horticulture,2016,40(12):92-96.[doi:10.11937/bfyy.201612023]
濒危植物羽叶丁香组织培养
- Title:
- Tissue Culture of Endangered Plant Syringa pinnatifolia
- Keywords:
- Syringa pinnatifolia; tissue culture; bud; seed
- 文献标志码:
- A
- 摘要:
- 以濒危植物羽叶丁香的芽和种子为外植体，采用植物组织培养法，研究了不同消毒方法对无菌体系建立、不同浓度的蔗糖和激素组合对种子初代培养、不同基本培养基和激素组合对增殖和生根的影响，以期为羽叶丁香的组培快繁、种质资源保护和开发利用提供依据。结果表明：新枝为较适宜的外植体，适宜的消毒方式为75%酒精消毒30 s，0.1%升汞消毒7 min，最适增殖培养基为MS+5.0 mg?L-1 6-BA+0.01 mg?L-1 IBA；最适种子初代培养基为MS+20 g?L-1蔗糖汁+3.0 mg?L-1 6-BA+0.10 mg?L-1 IBA，最适增殖培养基为MS+7.0 mg?L-1 6-BA+0.05 mg?L-1 IBA；最适生根培养基为WPM+2.0 mg?L-1 IBA。
- Abstract:
- The buds and seeds of endangered plant Syringa pinnatifolia were used as the test materials,the influence of different disinfection methods,concentration combinations of sucrose and hormone on primary culture of seeds,combinations of basal media and 〖JP5〗hormones on shoots proliferation and rooting by plant tissue culture method were studied,in order to provide basis for tissue culture propagation,germplasm resources protection and exploitation of Syringa pinnatifolia.The results showed that the suitable explants were shoots.The appropriate disinfection methods were 75% ethanol for 30 seconds,0.1% mercuric chloride for 7 minutes,the best proliferation medium was MS+5.0 mg?L-1 6-BA+0.01 mg?L-1 IBA;the optimal initial,proliferation and rooting medium for seed was MS+20 g?L-1 sucrose+3.0 mg?L-1 6-BA+0.10 mg?L-1 IBA,MS+7.0 mg?L-1 6-BA+0.05 mg?L-1 IBA and WPM+2.0 mg?L-1 IBA respectively according to this study.
参考文献/References:
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备注/Memo
第一作者简介：程明（1989-），男，湖北黄梅人，硕士，研究方向为园林植物遗传多样性和繁育。E-mail：962073977@qq.com.责任作者：蔡靖（1968-），女，陕西子州人，博士，教授，现主要从事森林植物及观赏植物和植物生理生态与森林生态等研究工作。E-mail：cjcaijing@163.com.基金项目：林业公益性行业科研专项资助项目（201204308）。