[1]乔艳明,陈文强,邓百万,等.秦巴山区香菇胞外多糖高产菌株的筛选[J].北方园艺,2015,39(08):137-142.[doi:10.11937/bfyy.201508036]
　QIAO Yan-ming,CHEN Wen-qiang,DENG Bai-wan,et al.Screening of Lentinula edodes with High Exopolysaccharide inQinling-Bashan Mountainous Area[J].Northern Horticulture,2015,39(08):137-142.[doi:10.11937/bfyy.201508036]
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秦巴山区香菇胞外多糖高产菌株的筛选

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年08 页码: 137-142 栏目: 出版日期: 2015-04-30
Title:
Screening of Lentinula edodes with High Exopolysaccharide inQinling-Bashan Mountainous Area
文章编号:
1001-0009（2015）08-0137-06
作者:
乔艳明1; 陈文强1; 2; 邓百万1; 2; 程贤利3; 彭浩1; 2; 王玉1
(1.陕西理工学院 生物科学与工程学院，陕西 汉中 723001；2.陕西省食药用菌工程技术研究中心，陕西 汉中 723001；3.汉中职业技术学院 药学与医学技术系，陕西 汉中 723000)
Author(s):
QIAO Yan-ming1; CHEN Wen-qiang1; 2 ; DENG Bai-wan1; 2 ; CHENG Xian-li3 ; PENG Hao1; 2 ; WANG Yu1
(1.School of Biological Science and Engineering，Shaanxi University of Technology，Hanzhong，Shannxi 723001；2.Shaanxi Engineering Research Center of Edible and Medicated Fungi，Hanzhong，Shannxi 723001；3.Department of Pharmaceutical and Medical Technology，Hanzhong Vocational and Technical College，Hanzhong，Shannxi 723000)
关键词:
香菇; 胞外多糖; 筛选; 液体发酵
Keywords:
Lentinula edodes; exopolysaccharide; screening; liquid fermentation
分类号:
S 646.1+2
DOI:
10.11937/bfyy.201508036
文献标志码:
A
摘要:
以秦巴山区主栽香菇菌株（808#、66#、68#）为试材，采用组织培养方法，用6种筛选培养基对其菌丝生长状况和胞外多糖产量进行了研究。结果表明：香菇66#菌株的菌丝在筛选培养基(葡萄糖1.0%、玉米粉2.0%、麦麸2.0%、酵母膏0.3%、KH2PO4 0.1%、MgSO4?7H2O 0.1%、pH自然)中的生长速度为3.26 mm/d，菌丝粗壮且致密；以此培养基进行液体发酵，其菌丝球数量多，大小均匀，生物转换量为66.00 g/L，胞外多糖产量可达2.99 g/L发酵清液。
Abstract:
Using tissue culture method，with Qinba Mountain main cultivated Lentinula edodes (808#，66#，68#) as test materials，Lentinula edodes with high exopolysaccharides was selected by 6 kinds of screening medium.The mycelial growth rate of Lentinula edodes strains and the exopolysaccharides production of Lentinula edodes with high exopolysaccharides were studied.The results showed that the hypha of Lentinula edodes (66#) was thick and dense and hypha growth rate was 3.26 mm/d in screening medium (glucose 1.0%，corn flour 2.0%，wheat bran 2.0%，yeast extract 0.3%，KH2PO4 0.1%，MgSO4? 7H2O 0.1%，pH naturally).There were many mycelia spheres to be evolved and the size of mycelia spheres was even，and the mycelia amount of biological transformation (wet weight) was up to 66.00 g/L in screening medium.The exopolysaccharides production of Lentinula edodes (66#) was up to 2.99 g/L.

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备注/Memo

第一作者简介：乔艳明(1990-)，男，硕士研究生，研究方向为微生物资源利用开发。E-mail：yanmingq1990@qq.com.

责任作者：陈文强(1956-)，男，教授，现主要从事微生物资源的保护与利用等研究工作。E-mail:wenqiangc@126.com.
基金项目：陕西省“13115”科技创新工程计划资助项目(2008IDGC-04)。

更新日期/Last Update: 2015-08-07