[1]张彩妮,邓百万,柏秋月,等.香菇胞外多糖及生物活性的对比[J].北方园艺,2021,(20):126-131.[doi:10.11937/bfyy.20211402]
　ZHANG Caini,DENG Baiwan,BAI Qiuyue,et al.Comparative Analysis of Extracellular Polysaccharide and Bioactivity Associated of Lentinus edodes[J].Northern Horticulture,2021,(20):126-131.[doi:10.11937/bfyy.20211402]
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香菇胞外多糖及生物活性的对比

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年20 页码: 126-131 栏目: 出版日期: 2021-10-30
Title:
Comparative Analysis of Extracellular Polysaccharide and Bioactivity Associated of Lentinus edodes
作者:
张彩妮1; 邓百万1; 2; 柏秋月1; 解修超1; 2
（1.陕西理工大学 生物科学与工程学院，陕西 汉中 723001；2.陕西理工大学 陕西省食药用菌工程技术研究中心，陕西 汉中 723001）
Author(s):
ZHANG Caini1; DENG Baiwan1; 2; BAI Qiuyue1; XIE Xiuchao1; 2
(1.School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723001;2.Shaanxi Provincial Engineering Research Center of Edible and Medicinal Fungi,Shaanxi University of Technology,Hanzhong,Shaanxi 723001)
关键词:
香菇; 胞外多糖; 抑菌; 抗氧化活性
Keywords:
Lentinus edodes; extracellular polysaccharide; antibacterial; antioxidant activity
DOI:
10.11937/bfyy.20211402
文献标志码:
A
摘要:
以L135、香66、808、日大4株香菇菌株为试材，采用苯酚-硫酸法、滤纸片扩散法及分光光度计法，研究了香菇胞外多糖含量、抑菌活性和抗氧化活性的差异，以期为香菇药食资源开发提供参考依据。结果表明：L135、808、香66、日大4株香菇的胞外多糖含量分别为（2.82±0.02）（1.61±0.04）（1.85±0.03）（2.22±0.06）mg?mL-1。当多糖质量浓度达到1.0 mg?mL-1时，菌株香66的还原能力最强，菌株日大的ABTS+自由基清除率最高，其清除率为39.97%；菌株L135的DPPH自由基清除率最高，其EC50值为3.53 mg?mL-1；菌株808的对羟自由基清除能力和抑菌活性最强。
Abstract:
L135,Xiang 66,808,Rida four strains of Lentinus edodes were used as the research objects.The contents of extracellular polysaccharides were determined by phenol-concentrated sulfuric acid method,the inhibition of bacteria was studied by filter paper diffusion method,and the antioxidant activity of polysaccharides in vitro was investigated by spectrophotometer,in order to provide reference for the development of Lentinus edodes medicinal and food resources.The results showed that the extracellular polysaccharide contents of Lentinus edodes L135,Xiang 66,808 and Rida were (2.82±0.02)mg?mL-1,(1.85±0.03)mg?mL-1,(1.61±0.04)mg?mL-1 and (2.22±0.06)mg?mL-1.When the concentration of Xiang 66 reached 1.0 mg?mL-1,the reduction ability of strain Xiang 66 were better than those of other three strains,the scavenging rate of strain Rida to ABTS+ radicals was 39.79%,higher than that of the other three strains,DPPH? scavenging rate of strain L135 were better than those of the other three strains,with EC50 of 3.53 mg?mL-1,respectively,the scavenging rates of hydroxyl free radical by 808 were higher than the others,and the antimicrobial activity of exopolysaccharide from strain 808 was higher than that of the other three agaricus blazei strains.

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备注/Memo

第一作者简介：〖JP3〗张彩妮（1994-），女，硕士研究生，研究方向为微生物资源保育与开发利用。E-mail:984365864@qq.com.责任作者：邓百万（1963-），男，硕士，教授，现主要从事微生物资源保育与开发利用等研究工作。E-mail:2210309868@qq.com.基金项目：陕西省重点研发计划资助项目（2017ZDXM-NY-001）。收稿日期：2021-03-31

更新日期/Last Update: 2022-02-10