[1]贺伟强,巢新冬,白杰.香菇中多酚类物质提取工艺的优化研究[J].北方园艺,2013,37(18):125-128.
　HEWei-qiang,CHAOXin-dong,BAIJie.StudyontheOptimizationoftheExtractionTechnologyof TotalPhenolicfromLentinulaedodes[J].Northern Horticulture,2013,37(18):125-128.
点击复制

香菇中多酚类物质提取工艺的优化研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年18期 页码: 125-128 栏目: 出版日期: 2013-09-30
Title:
StudyontheOptimizationoftheExtractionTechnologyof TotalPhenolicfromLentinulaedodes
文章编号:
1001-0009(2013)18-0125-04
作者:
贺伟强; 巢新冬; 白杰
嘉兴职业技术学院，浙江嘉兴314036
Author(s):
HEWei-qiang; CHAOXin-dong; BAIJie
JiaxingVocationalandTechnicalCollege,Jiaxing,Zhejiang314036
关键词:
香菇; 多酚; 响应面分析法; 优化
Keywords:
Lentinulaedodes; totalphenolic; responsesurfacemethodology; optimization
分类号:
S646.1+2
文献标志码:
A
摘要:
以香菇子实体为原料，采用Plackett-Burman设计对影响香菇多酚提取效率的8个因素进行筛选，并通过Box-Behnken设计及响应面分析法确定了影响多酚类提取因素的最佳条件。结果表明：影响香菇多酚提取的3个主要因素为：乙醇浓度、液料比和提取温度；最佳提取条件为：香菇过160目筛，以81%的乙醇作溶剂，液料比为34∶1mL/g，先超声处理15min，在85℃的水浴中提取45min，提取3次。根据最优试验条件对模型进行验证，得到香菇子实体的多酚提取量为186.50mg/100gFW，实际值与预测值的误差为3.24%。
Abstract:
TakingthefruitofLentinusedodesasmaterial,Plackett-BurmandesignwasusedtoevaluatetheinfluenceofrelatedeightfactorsoftotalphenolicfromLentinusedodes.AndtheBox-Behnkendesignandresponsesurfaceanalysiswereusedtodeterminetheoptimallevelsofthemainfactors.Theresultsshowedthatthreefactorsplayedtheimportantrolesinthemedium,includingethanolconcentration,temperatureandsolventsolidratio.Theoptimalconditionswereparticlesizelessthan160pore,15minforultrasonictime,34∶1mL/gforsolventsolidratio,81%ethanolforsolution,extractedat85℃for45min,extractingfor3times.Undertheoptimalconditions,theyieldoftotalphenolicreached186.50mg/100gFW,only3.24%differencesfromtheforecastbyRSM.

参考文献/References:

[1]中华人民共和国农业部农产品加工局.中国农产品加工业发展报告[M].北京:中国农业科学技术出版社,2007:115-116.
[2]王艺峰,徐敏,洪群峰,等.羧甲基化香菇多糖对壳聚糖表面的化学修饰[J].高分子材料科学与工程,2011,27(8):109-112.
[3]钟耀广,林楠,王淑琴,等.香菇多糖的抗氧化性能与抑菌作用研究[J].食品科技,2007(7):141-144.
[4]冯燕,全吉钟,王冬旭,等.香菇多糖抗胃癌机制研究[J].中国试验诊断学,2011(15):1829-1831.
[5[ProestosC,BoziarisIS,NychasGJE,etal.AnalysisofflavonoidsandphenolicacidsinGreekaromaticplants:investigationoftheirantioxidantcapacityandantimicrobialactivity[J].FoodChemistry,2004,95:664-671.
[6]YoshimotoM,OkunoS,YamaguchiM,etal.Antimutagenicityofdeacylatedanthocyaninsinpurplesweetpotato[J].BioscienceBiotechnologyandBiochemistry,2001,65:1652-1655.
[7]CookNC,SammanS.Flavonoids-chemistry,metabolism,cardioprotectiveeffects,anddietarysources[J].JournalofNutritionBiochemistry,1996(7):66-76.
[8]Rice-EvansCA,MillerNJ,PagangaG.Antioxidantpropertiesofphenoliccompounds[J].TrendsinPlantScience,1997(2):152-159.
[9]吕国英,张作法,潘慧娟,等.响应面分析法优化香菇多糖发酵培养基[J].菌物学报,2010,29(1):106-112.
[10]WallsI,ChuyateR.Alicyclobacillus-historicalperspectiveandpreliminarycharacterizationstudy[J].DairyFoodandEnvironmentalSanitation,1998,18:499-503.
[11]陈召国,杨文革,胡永红,等.响应面法优化猪硫酸软骨素的提取工艺[J].食品科技,2011,36(7):187-192.
[12]谭玉柱,童婷婷,谢运飞,等.Plackett-Burman试验设联用星点设计效应面法优选大黄地上部位蒽醌类成分提取工艺[J].中国药学杂志,2012,47(6):410-414.
[13]BalusuR,PaduruRR,KuraviS,etal.OptimizationofcriticalmediumcomponentsusingresponsesurfacemethodologyforethanolproductionfromcellulosicbiomassbyClostridiumthermocellumSS19[J].ProcessBiochemistry,2005,40:3025-3030.
[14]ShiLE,YingGQ,ZhangXY,etal.Mediumoptimizationfor5’-phosphodiesteraseproductionfrompenicilliumcitrinumusingresponsesurfacemethodology[J].FoodTechnologyandBiotechnology,2007,45:126-133.
[15]NadiaA,SolimanMM,BerekaaYR,Polyglutamicacid(PGA)productionbyBacillussp.SAB-26:applicationofPlackett-Burmanexperimentaldesigntoevaluateculturerequirements[J].AppliedMicrobiologyandBiotechnology,2005,69:259-267.

相似文献/References:

[1]郭 丽,王 鹏,马志婷.香菇固态发酵对蓝莓果渣产物活性成分的研究[J].北方园艺,2014,38(11):143.
　GUO Li,WANG Peng,MA Zhi-ting.Study on Active Ingredient of Blueberry Pomace in Solid-state Fermentation by Lentinus edodes [J].Northern Horticulture,2014,38(18):143.
[2]郑 涛,苏 锐,赵韵美,等.青杄针叶多酚超声提取工艺的优化[J].北方园艺,2013,37(22):138.
　ZHENG Tao,SU Rui,ZHAO Yun-mei,et al.Optimization of Ultrasonic Extraction of Polyphenols from the Leaves of Picea wilsonii[J].Northern Horticulture,2013,37(18):138.
[3]邹家丽,邓骛远,和七一,等.山茶花中多酚提取的方法及其抗氧化活性测定研究[J].北方园艺,2013,37(13):103.
　ZOU Jia-li,DENG Wu-yuan,HE Qi-yi,et al.Polyphenol Extracted from the Camellia japonica Flowers and Its Antioxidant Activity[J].Northern Horticulture,2013,37(18):103.
[4]刘伟,杨如箴,李紫薇,等.响应面法优化新疆野酸梅果肉多酚提取工艺研究[J].北方园艺,2014,38(04):123.
　LIU Wei,YANG Ru-zhen,LI Zi-wei,et al.Optimization of Polyphenol From Xinjiang Wild Plum Flesh Using Response Surface Methodology[J].Northern Horticulture,2014,38(18):123.
[5]王新红,吴秀红,左玉香.紫茉莉种外皮多酚提取工艺的响应面法优化研究[J].北方园艺,2013,37(07):147.
　WANG Xin-hong,WU Xiu-hong,ZUO Yu-xiang.Study on Optimization of Extraction Process by Response Surface from Total Phenol of Mirabilis jalapa Seed Epicarp[J].Northern Horticulture,2013,37(18):147.
[6]徐彦军,吴秀泉,任志华,等.贵州山区香菇菌株栽培比较试验[J].北方园艺,2013,37(06):153.
　XU Yan-jun,WU Xiu-quan,REN Zhi-hua,et al.Cultivation Experiment of Lentinus edodes Strains in Guizhou Mountainous Area[J].Northern Horticulture,2013,37(18):153.
[7]张俊环,杨 丽,孙浩元,等.不同品种杏果实发育进程中多酚与类黄酮物质含量的变化[J].北方园艺,2012,36(24):1.
　ZHANG Jun-huan,YANG Li,SUN Hao-yuan,et al.Changes of the Total Phenols and Flavonoids in Apricot Peel and Pulp of Different Cultivars During Fruit Development[J].Northern Horticulture,2012,36(18):1.
[8]李盛旻,王永宏,王广耀.香菇山楂复合果丹皮的研制[J].北方园艺,2012,36(21):126.
　LI Sheng-min,WANG Yong-hong,WANG Guang-yao.Development of Mushrooms the Hawthorn Composite Sweetend Roll[J].Northern Horticulture,2012,36(18):126.
[9]赵志顺,逄振茹.辽宁东部山区人工栽培香菇过程中畸形菇发生原因及预防[J].北方园艺,2013,37(18):143.
　ZHAOZhi-shun,PANGZhen-ru.CausesandPreventionofDeformedFruitingBodyintheProcessofArtificialCultavitionof LentinusedodesintheMountainousAreasofEasternLiaoning[J].Northern Horticulture,2013,37(18):143.
[10]赵娜娜,陈文强,邓百万,等.秦巴山区香菇主要栽培种亲缘关系的研究[J].北方园艺,2014,38(16):140.
　ZHAO Na-na,CHEN Wen-qiang,DENG Bai-wan,et al.Study of the Genetic Relationships of Main Lentinula edodes Cultispecies in Qinba Mountainous Area[J].Northern Horticulture,2014,38(18):140.

备注/Memo

第一作者简介：贺伟强（1979-），男，河南郑州人，硕士，讲师，现主要从事天然活性成分的提取和分离技术等研究工作。
基金项目：嘉兴市科技计划资助项目（2012AY1058）。
收稿日期：2013-04-15

更新日期/Last Update: 2014-09-09