CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(13):106-109.
三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究
- Title:
- Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon
- 文章编号:
- 1001-0009(2014)13-0106-04
- Keywords:
- triploid muskmelon; tissue culture; immature embryo; adventitious bud
- 分类号:
- S 652
- 文献标志码:
- A
- 摘要:
- 以三倍体薄皮甜瓜授粉后30 d未成熟胚为试材,采用组织培养的胚培养方法,对三倍体薄皮甜瓜芽的再生进行了研究。结果表明:当TDZ浓度为0.04 mg/L时,未成熟胚接种第5天出芽率就达到最大值,出芽整齐一致。在M5激素配比组合(6-BA 1.0 mg/L,IAA 0.04 mg/L)培养基中,平均再生芽数最高,芽的质量最好。在S2(6-BA 0.05 mg/L)培养基中,芽伸长量最多,生长状态最好。
- Abstract:
- Taking immature embryos of the triploid muskmelon which grew 30 days after pollination as material,using tissue culture of embryo culture,the regeneration buds of triploid muskmelon were studied.The results showed that in the budding induced immature embryo,the highest seedling rate was obtained on MS medium containing TDZ 0.04 mg/L after five days.The highest frequency shoot regeneration was obtained on MS medium containing 6-BA 1.0 mg/L,IAA 0.04 mg/L.The quality of buds was the beat,the adventitious shoots transferred into MS medium containing 6-BA 0.05 mg/L,the buds elongated more and grew best.
参考文献/References:
[1]金荣荣,陈柏杰,汪磊,等.三倍体薄皮甜瓜新品种哈甜3号的选育[J].中国蔬菜,2011(10):95-97.
[2]Niedz R P,Schiller S S,Dunbar K B,et al.Factors influencing shoot regeneration from cotyle-donary explants of Cucumis melo[J].Plant Cell Tissue and Organ Culture,1989,18(3):313-319.
[3]Kathal R,Bhatnagar S P,Bhojwani S S.Plant re-generation from the callus derived from root ex-plants of Cucumis melo L.cv Pusa Sharbati[J].Plant Science (Limerick),1994,96(1-2):137-142.
[4]Tabei Y,Nishio T,Kanno T.Shoot regenerationfrom cotyledonary protoplasts of melon (Cucumis melo L.cv Charentais) [J].Journal of the Japanese Society for Horticultural Science,1992,61(2):317-322.
[5]Leshem B.Polarity and responsive regions for regen-eration in the cultured melon cotyledon[J].Journal of Plant Physiology,1989,135(2):237-239.
[6]邓向东,耿玉轩,路子显,等.外植体和培养基因子对哈蜜瓜不定芽诱导的影响[J].园艺学报,1996(1):57-61.
[7]傅振清.哈密瓜幼胚离体培养初报[J].新疆农业科学,1985(3):27-28.
[8]方智远,孙培田,刘玉梅.萝卜与甘蓝远缘杂交研究初报[J].园艺学报,1983,10(3):187-191.
[9]盛慧.黄瓜单倍体培育及其发育相关基因筛选[D].哈尔滨:东北农业大学,2010.
[10]张永兵,陈劲枫,尹鸿平,等.辐射花粉授粉诱导甜瓜单倍体[J].果树学报,2006,23(6):892-895.
[11]雷春,陈劲枫,钱春桃,等.辐射花粉授粉和胚培养诱导产生黄瓜单倍体植株[J].西北植物学报,2004,24(9):1739-1743.
[12]Ziv M,Meir G,Halevy,A.H.Faetors influeneing the Production of ardened glaucous camation plantlets in vitro[J].Plant Cell Tissue organ Culture,1983(2):55-65.
[13]夏海武,吕柳新,夏天,等.弥河银瓜高效植株再生体系的建立[J].植物生理学通讯,2006,42(2):235-238.
[14]杨丽琴,李瑞,王俊,等.植物组织培养的三大难题[J].北方园艺,2008(4):104-107.
[15]陶兴林,黄永红,陆璐,等.两个甜瓜品种高效再生体系的建立[J].西北植物学报,2005,25(4):806-811.
[16]冯凤娟,梁东,马锋旺,等.甜瓜叶片高效再生体系的建立[J].西北农业学报,2008,7(5):321-324.
[17]潘俊松,蔡润,刘晓,等.甜瓜子叶节培养高效再生体系建立[J].上海交通大学学报(农业科学版),2003,21(4):295-298.
[18]孙天国,沙伟,金忠民.薄皮甜瓜子叶组织培养的研究[J].北方园艺,2005(2):64-65.
相似文献/References:
[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(13):80.
[4]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica(Fisch.)Spreng[J].Northern Horticulture,2014,38(13):84.
[5]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(13):101.
[6]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(13):89.
[7]赵丽莉,徐芳芳,荀 洋,等.桔梗花药愈伤组织诱导培养基筛选[J].北方园艺,2014,38(01):95.
ZHAO Li-li,XU Fang-fang,XUN Yang,et al.Screening of Callus Induction Medium of Platycodon grandiflorumAnther[J].Northern Horticulture,2014,38(13):95.
[8]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(13):106.
[9]陈丽文,杨利平,荣薏,等.金心也门铁工厂化育苗技术研究[J].北方园艺,2013,37(13):138.
CHEN Liwen,YANG Li-ping,RONG Yi,et al.Study on the Techniques of Industrialized Seedling Culture of Draceana arborea[J].Northern Horticulture,2013,37(13):138.
[10]申顺先,李学慧,贺爱利,等.留兰香微繁技术体系研究[J].北方园艺,2013,37(13):146.
SHEN Shun-xian,LI Xue-hui,HE Ai-li,et al.Study on Micropropagation Technique System of Mentha spicata Linn[J].Northern Horticulture,2013,37(13):146.
备注/Memo
第一作者简介:曹虹(1982-),女,硕士,农艺师,现主要从事甜瓜育种等研究工作。E-mail:45823073@qq.com.