CHEN Chun-hua,GAO Jian-ming,LIU Qiao-lian,et al.Study on Tetraploid Induction,Identification and Biological Characteristics of Asparagus[J].Northern Horticulture,2014,38(11):88-92.
芦笋四倍体诱导、倍性鉴定及生物学特性的研究
- Title:
- Study on Tetraploid Induction,Identification and Biological Characteristics of Asparagus
- 文章编号:
- 1001-0009(2014)11-0088-05
- 分类号:
- S 644.6
- 文献标志码:
- A
- 摘要:
- 以南方主栽品种“井岗701”二倍体芦笋的露白种子和幼苗为试材，研究了秋水仙素浓度、处理时间及浸泡时的转速对芦笋四倍体诱导效果的影响，并对四倍体和二倍体芦笋的部分抗性和形态特征进行了比较。结果表明：以0.2%的秋水仙素浸泡露白种子6 h效果最好，四倍体诱导率最高，为20%，但浸泡时的不同转速对四倍体诱导影响差异不显著；而用0.3%的秋水仙素连续滴苗4 d，四倍体诱导率最高为18%；浸泡露白种子法比滴苗法诱导效果好；四倍体植株较二倍体更加粗壮，在抗热性和抗旱性方面也明显优于二倍体。
- Abstract:
- Taking the bourgeoning seeds and seedlings of‘Jingang 701’asparagus (Asparagus officinalis L.) which had the main cultivar in southern China as test material,effect of colchicine concentration,treatment time and centrifugal speed on induced tetraploid were studied,and the morphological and resistance of tetraploid and diploid asparagus were compared.The results showed that 0.2% colchicine soaking seeds 6 h had the best effect,the highest frequency of induction was 20%.But there was no significant effect of different centrifugal speed under soaking.When the colchicine concentration was 0.3% and the injection time was 4 d,the frequency of induction was 18%.The method of bourgeoning seeds dipping with colchicine solution was better than the seedling injection method.Comparing with diploid asparagus,the stem of tetraploid asparagus was much bigger and the resistance to heat and drought was higher than that of the diploid asparagus.
参考文献/References:
[1] 孙春艳,赵伯涛,郁志芳,等.芦笋的化学成分及药理作用研究进展[J].中国野生植物资源,2004(5):1-5.
[2] 林友胜,胡松青.芦笋的药理和临床研究述要[J].辽宁中医学院学报,2005(6):637-638.
[3] 何智峰.芦笋果肉营养成分的分析[J].食品工业科技,2008(3):271-275.
[4] 叶春勇,林媚.芦笋营养成分分析研究[J].中国果菜,2005(2):37-38.
[5] 王嘉彦.芦笋糖浆对恶性肿瘤化疗增效减毒作用的临床观察[J].中医药学报,1996(4):26-27.
[6] 郑思乡,李利良,甘红霞,等.芦笋多倍体诱导及其离体培养的研究[J].湖南农业科学,1996(1):22-23.
[7] 王平,张韶岩.日本芦笋多倍体育种的进展[J].山东农业科学,1989(4):50-51.
[8] 张天翔,林宗铿,蔡坤秀,等.我国芦笋育种研究进展[J].江西农业学报,2011（4）:57-59.
[9] 吴繁花.热研五号柱花草同源四倍体诱导的研究[D].海口:海南大学,2009.
[10] 宗红.大葱组织培养及其多倍体诱导的初步研究[D].泰安:山东农业大学,2007.
[11] 张兴翠,周昌华,殷家明.药用百合的多倍体诱导及快速繁殖[J].西南农业大学学报,2003,25(1):15-17.
[12] 葛志东.氟乐灵诱导西葫芦染色体加倍研究[J].中国农学通报,2009,25(8):215-218.
[13] Hansen N J P,Andersen S B.In vitro chromosome doubling potential of colchicine,oryzalin,trifluralin,and APM in Brassica napus microspore culture[J].Euphytica,1996,88(2):159-164.
[14] 尚爱芹,张成合,刘世雄,等.菜心多倍体诱变及其细胞学观察[J].河南科学,1999,17(6):6-9.
[15] 宋平根、李索文.流式细胞术的原理和应用[M].北京:北京师范大学出版社,1992.
[16] 施先锋.西瓜多倍体诱导及倍性鉴定的研究[D].武汉:华中农业大学,2007.
[17] 张天翔,林宗铿,蔡坤秀,等.白芦笋花药培养再生植株染色体倍性检测研究[J].福建热作科技,2008(1):11-13.
[18] 张天翔,林宗铿,杨俊杰,等.芦笋试管苗根尖染色体制片技术研究[J].福建热作科技,2008(2):10-11.
[19] 齐永顺.葡萄四倍体及其生物学研究[D].保定:河北农业大学,2009.
[20] 胡秀.离体培养条件下金鱼草四倍体切花的诱导及培育[J].云南农业大学,2009,19(5):524-527.
[21] 唐艳.非洲紫罗兰的四倍体诱导及其特性分析[D].重庆:西南大学,2010.
[22] 邹道谦,崔丽华.秋水仙素诱导四倍体石刁柏的研究[J].河北农业大学学报,1990,1(13):29-33.
[23] 沈河溪.秋水仙素诱导柠条中间锦鸡儿多倍体的研究[D].重庆:西南大学,2011.
[24] 陈发棣,蒋甲福,房伟民.秋水仙素诱导菊花脑多倍体的研究[J].上海农业学报,2002,18(1):46-50.
[25] 张天翔,林宗铿,蔡坤秀,等.芦笋单倍体染色体加倍技术研究[J].中国农学通报,2011,27(13):212-215.
[26] 康喜亮,郝秀英,刘敏,等.秋水仙素诱导天山雪莲四倍体[C].全国第九届天然药物资源学术研讨会论文集,2010:406-410.
[27] 周玥玥.五节芒再生体系的建立与多倍体的诱导研究[D].长沙:湖南农业大学,2012.
[28] 杨今后,扬新华.桑叶气孔保卫细胞叶绿体数与染色体倍数性关系的测定[J].浙江农业科学,1990(5):238-240.
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备注/Memo
第一作者简介：陈春桦（1989-），女，硕士研究生，研究方向为芦笋多倍体育种。
责任作者：易克贤（1964-），男，博士，研究员，研究方向为热带作物抗病遗传育种及热带作物真菌病害。E-mail：yikexian@126.com.
基金项目：国家公益性行业(农业) 科研专项资助项目（201003074）。
收稿日期：2014-02-10