SHI Min,YANG Hong-yu,ZHANG Guo-bin,et al.Tissue Culture and Rapid Propagation of Dendranthema morifolium‘Qiandaiji’[J].Northern Horticulture,2014,38(20):105-108.
菊花“千代姬”组培快繁条件优化
- Title:
- Tissue Culture and Rapid Propagation of Dendranthema morifolium‘Qiandaiji’
- 文章编号:
- 1001-0009（2014）20-0105-04
- 分类号:
- S 682.21
- 文献标志码:
- A
- 摘要:
- 以菊花“千代姬”的叶片为外植体，对其愈伤组织的诱导、丛生芽的增殖和生根的诱导等不同过程的各种培养基和培养条件进行了研究。结果表明：愈伤组织诱导最适培养基为SH+0.5 mg/L 6-BA+1.0 mg/L NAA，其诱导率为100%。丛生芽增殖培养基为MS+2.0 mg/L 6-BA+0.2 mg/L NAA，诱导生根的培养基为1/2MS+0.1 mg/L NAA，3类培养基均加蔗糖30 g/L，琼脂6.0 g/L，在pH 5.8、光照强度2 500 lx、温度25℃、光照时间12 h/d的条件下培养最适宜。
- Abstract:
- Taking leaf of Chrysanthemum morifolium ‘Qiandaiji’ as material,tissue culture conditions and media for callus induction,cluster buds propagation and rooting were studied in this article.The results showed that the proper medium of callus induction was SH+0.5 mg/L 6-BA+1.0 mg/L NAA and resulted in an induction efficiency of 100%.The cluster buds well grow in MS+2.0 mg/L 6-BA+0.2 mg/L NAA.And the appropriate rooting medium was 1/2MS+0.1 mg/L NAA.All three types of media were contained 30 g/L sucrose and 6.0 g/L agar with pH 5.8.The culture conditions were optimized to 2 500 lx light intensity,25℃ temperature and 12 h light/12 h dark circle.
参考文献/References:
[1]戴思兰.中国菊花史略[J].湖南林业,2007(11):31.
[2]陈俊愉.中国菊花过去和今后对世界的贡献[C]//中国风景园林学会菊花研究专业委员会.2007中国（中山小榄）国际菊花研讨会论文集.中国风景园林学会菊花研究专业委员会,2007:6.
[3]齐向英,郑丹,张超,等.菊花组织培养研究[J].江苏农业科学,2009(5):63-64.
[4]肖志坚,纪艳,刘德江,等.菊花的组织培养技术[J].园艺与种苗,2012(3):21-23.
[5]周洲,李永丽,姜玲,等.菊花‘绿鹦哥’的组织培养和快速繁殖[J].北方园艺,2009(10):110-112.
[6]陈黎,万志兵,曹玲玲.黄山贡菊组织培养与快速繁殖技术研究[J].黄山学院学报,2013(5):63-65.
[7]张月娇.菊花“神马”组织培养快繁技术研究[J].林业勘察设计,2012(1):139-142.
[8]袁成志,李波,杨蔚然,等.激素对菊花愈伤组织诱导和丛生芽分化的影响[J].北方园艺,2010(1):162-164.
[9]于红芳,许刚,李永华,等.菊花品种唐宇金秋组织培养研究[J].河南农业科学,2009(11):114-117.
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备注/Memo
第一作者简介：施敏（1989-），女，硕士研究生，研究方向为园林植物。E-mail:shimin19891117@126.com.
责任作者：鄢波（1967-），男，博士，教授，研究方向为植物生物技术。E-mail:yanbodr@aliyun.com.
基金项目：国家自然科学基金资助项目(31060042；31260062)。
收稿日期：2014-04-25