LU Xing-xia,CHAI Ci-jiang,ZHANG Ting,et al.Study on the Vitrification of Cotoneaster hjelmqvistii Plantlet in vitro[J].Northern Horticulture,2014,38(18):103-106.
栒子试管苗玻璃化影响因素的研究
- Title:
- Study on the Vitrification of Cotoneaster hjelmqvistii Plantlet in vitro
- 文章编号:
- 1001-0009（2014）18-0103-04
- 分类号:
- Q 949
- 文献标志码:
- A
- 摘要:
- 以栒子试管苗为试材，分别研究了不同的培养瓶封口物、不同6-BA浓度（0~0.90 mg/L）、不同琼脂浓度（6.5~8.5 g/L）及不同培养温度对栒子试管苗玻璃化的影响。结果表明：当6-BA浓度为0.30 mg/L、琼脂浓度为6.5 g/L、培养温度23~24℃，以棉塞或封口膜封口时，栒子试管苗玻璃化率较低，繁殖系数较高。
- Abstract:
- Taking Cotoneaster hjelmqvistii plantlet in vitro as materials，the effect of coverings of the vessels，6-BA concentrations (0~0.90 mg/L)，agar concentrations (6.5~8.5 g/L) and shoots culture temperature on its vitrification phenomenon were studied.The results showed that the vitrification rate was lower，and the propagation coefficient was higher when the Cotoneaster hjelmqvistii plantlet was cultured on MS basal salts containing 0.30 mg/L 6-BA and 6.5 g/L agar concentration，in 23~24℃ cultured temperature and in the vessels covering with cotton plug or sealing film.
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备注/Memo
第一作者简介：卢兴霞(1978-)，女，硕士，讲师，研究方向为观赏植物遗传育种与生物技术。E-mail:luxingxia_@163.com.
责任作者：柴慈江(1960-)，男，硕士，教授，研究方向为植物组培技术研究。E-mail:cijiang666@163.com.
基金项目：国家级星火计划资助项目（2008GA610015，2013GA610012）；天津市科委资助项目(08ZHXHNC07000)。收稿日期：2014-04-24