WANG Qian,MA Xu-guang,LIU Min,et al.Selection for a Hericium erinaceus Mutant Strain in the Way of Protoplasts Being Exposed to the UV Radiation[J].Northern Horticulture,2014,38(16):145-149.
原生质体紫外诱变选育猴头菌株
- Title:
- Selection for a Hericium erinaceus Mutant Strain in the Way of Protoplasts Being Exposed to the UV Radiation
- 文章编号:
- 1001-0009(2014)16-0145-03
- 分类号:
- S646
- 文献标志码:
- A
- 摘要:
- 以河北大学食药用真菌研究所保藏的猴头（Hericium erinaceus）He001菌株为试材，对其进行原生质体紫外诱变处理，共获得273株再生菌株，初筛获得14株再生菌株与出发菌株有明显拮抗现象，进一步筛选出8株再生菌株菌丝长速优于出发菌株，并通过酯酶同工酶电泳技术对该8株菌株进行菌种真实性鉴定。结果表明：8株菌株共检测到4谱带，分布于迁移率在0.41～0.59之间；H035与出发菌株的联合系数为0.33，与He001遗传差异最大；同时它在栽培常用培养基上长速优于出发菌株，差异达到了极显著水平，因此初步认定其为优良的再生变异菌株。
- Abstract:
- Use Hericium erinaceus He001 that stored in Institute of Medicinal Fungi of Heibei University,273 strains were regenerated in the process of protoplasts of He001 being exposed to the UV radiation.14 strains were filtered out based on the mycelial antagonistic symbols,and 8 strains,of which mycelial growth rates were higher than the original,were chosen to detect their esterase isozyme to confirm whether they were genetically different from the original.The results showed that there were four bands of esterase isozyme located in the range of the migration rate between 0.41 and 0.59 in all.The association coefficient between H035 and He001 was 0.33,which was the smallest and meant H035 was genetically different from He001.Besides,the mycelia growth rate of strain H035 was higher than He001,and the difference was extremely significant.On the basis of all facts above,H035 was confirmed to be a mutant strain.
参考文献/References:
［1］任大明,杜志强,李秀娜,等.猴头菌丝多糖免疫调节和抗氧化功能研究［J］.沈阳农业大学学报,2007,38(3):370-374.
相似文献/References:
[1]张金鹏,杨 晶,韩玉珠,等.植物体细胞杂交的研究进展[J].北方园艺,2014,38(01):192.
ZHANG Jin-peng,YANG Jing,HAN Yu-zhu,et al.Research Progress of Plant Somatic Hybridization[J].Northern Horticulture,2014,38(16):192.
[2]邓红梅,马超,王颖.黄瓜幼苗子叶原生质体的分离纯化及融合条件优化研究[J].北方园艺,2013,37(11):6.
DENG Hong-mei,MA Chao,WANG Ying.Study on the Optimal of Separation,Purification and Fusion Conditions of Cucumber Seedlings Cotyledons Protoplast[J].Northern Horticulture,2013,37(16):6.
[3]徐琴,王晓军,王卉,等.苘麻叶肉原生质体的分离和培养研究[J].北方园艺,2013,37(07):170.
XU Qin,WANG Xiao-jun,WANG Hui,et al.Study on Isolation and Culture of the Mesophyll Protoplasts from Abutilon avicennae[J].Northern Horticulture,2013,37(16):170.
[4]王昱,王义,王康宇,等.灵芝原生质体的制备与再生研究[J].北方园艺,2013,37(16):184.
WANGYu,WANGYi,WANGKang-yu,et al.StudyonPreparationandRegenerationoftheProtoplastsfrom Ganodermalucidum[J].Northern Horticulture,2013,37(16):184.
[5]柳焕章,卢月霞,郑素月.四种食药用真菌菌种扩繁培养基筛选[J].北方园艺,2014,38(17):145.
LIU Huan-zhang,LU Yue-xia,ZHENG Su-yue.Screening of Culture Medium of Four Different Edible-medicinal Fungi[J].Northern Horticulture,2014,38(16):145.
[6]王卉,宁慧霞,刘敏,等.莴苣原生质体的分离方法[J].北方园艺,2012,36(11):109.
WANGHui,NINGHui-xia,LIUMin,et al.StudyontheMethodofIsolationProtoplastsofLactucasativaL.[J].Northern Horticulture,2012,36(16):109.
[7]肖 望,涂红艳,邓崇会.红姜花胚性细胞悬浮体系的建立和原生质体培养[J].北方园艺,2015,39(05):104.[doi:10.11937/bfyy.201505032]
XIAO Wang,TU Hong-yan,DENG Chong-hui.Establishment of Embryogenic Cell Suspension and Protoplast Culture of Hedychium coccineum[J].Northern Horticulture,2015,39(16):104.[doi:10.11937/bfyy.201505032]
[8]温 海 祥,肖 洪 东,黄 剑 波,等.草菇种内原生质体融合育种研究[J].北方园艺,2000,24(03):0.[doi:10.11937/bfyy.200003037]
[J].Northern Horticulture,2000,24(16):0.[doi:10.11937/bfyy.200003037]
[9]杨珊,张赫男,李巧珍,等.猴头菌融合育种原生质体制备与再生条件优化[J].北方园艺,2018,42(09):159.[doi:10.11937/bfyy.20173296]
YANG Shan,ZHANG Henan,LI Qiaozhen,et al.Optimization of Protoplast Preparation and Regeneration Conditions of Hericium erinareus Fusion Breeding[J].Northern Horticulture,2018,42(16):159.[doi:10.11937/bfyy.20173296]
[10]杨佳乐,范桂枝,张李香.秋水仙素诱变对桑黄菌丝生长及活性成分的影响[J].北方园艺,2021,(07):110.[doi:10.11937/bfyy.20204446]
YANG Jiale,FAN Guizhi,ZHANG Lixiang.Effects of Colchicine Mutagenesis on Growth and Active Ingredients in Hyphae of Phellinus igniarius[J].Northern Horticulture,2021,(16):110.[doi:10.11937/bfyy.20204446]
备注/Memo
.第一作者简介：王谦（1962-），男，本科，研究员，现主要从事食药用真菌研究与开发工作。E-mail：wq6203_cn@126.com.