ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(22):101-103.
药用植物玛咖离体快繁技术研究
- Title:
- Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii
- 文章编号:
- 1001-0009(2013)22-0101-03
- 分类号:
- R 931.2
- 文献标志码:
- A
- 摘要:
- 以玛咖无菌苗的幼根、胚轴、子叶、带芽茎段为外植体，以MS培养基为基本培养基，研究了不同浓度激素组合对愈伤组织诱导、芽簇诱导及生根的影响，以期筛选最佳激素组合及浓度配比。结果表明：NAA、2,4-D与6-BA组合有利于玛咖幼根愈伤组织及带芽茎段芽簇的诱导，单独使用NAA和IBA可诱导玛咖再生植株生根。最佳愈伤组织、芽簇及生根诱导培养基分别为MS+NAA 0.5 mg/L+6-BA 1.0 mg/L、MS+NAA 0.25 mg/L+6-BA 2.0 mg/L、MS+NAA 0.5 mg/L。
- Abstract:
- Taking the shoot tip,radical,cotyledons,stem with bud of Lepidium meyenii as explants,with MS as basic medium,the effect of different concentrations of hormone combinations on callus induction,proliferation,and seedling root were studied,in order to select an optimum hormone combination and concentration ratio.The results showed that the combination of NAA,2,4-D and 6-BA were helpful to induce the callus and stem with buds regeneration of Lepidium meyenii.Just adding NAA or IBA was contribute to the induction of renewable rooting.The best formula for callus induction,bud clusters and rooting were MS+NAA 0.5 mg/L+6-BA 1.0 mg/L,MS+NAA 0.25 mg/L+6-BA 2.0 mg/L,MS+NAA 0.5 mg/L.
参考文献/References:
[1] 余龙江,金文闻,吴元喜,等.玛咖的植物学及其药理作用研究概况[J].天然产物研究与开发,2002,14(5):71-74.
[2] 余龙江,孙友平,程华,等.玛咖引种气候适宜区域的选择[J].生命科学研究,2004,8(3):250-255.
[3] 冯颖,何钊,徐珑峰,等.云南栽培玛咖的营养成分分析与评价[J].林业科学研究,2009,22(5):696-700.
[4] 邵敏,和献勤,和丽芬,等.不同肥料对玛咖生长及产量的影响研究[J].云南农业科技,2009(4):20-21.
[5] 程华,余龙江,孙友平,等.玛咖的愈伤组织诱导及植株再生[J].植物生理学通讯,2004,40(6):709.
[6] 刘忠荣,陈屏昭.植物组织培养技术在观赏植物中的应用[J].昭通师范高等专科学校学报,2003,25(5):41-43.
[7] 刘良宏,李斌,胡广益,等.植物组织培养产业化生产技术要点[J].中国园艺文摘,2009(4):138-140.
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备注/Memo
第一作者简介：朱军（1982-），男，硕士，助理研究员，研究方向为药用植物组织培养及栽培。E-mail:zhujun1hao@163.com.
责任作者：李晓瑾（1962-），女，硕士，研究员，硕士生导师，研究方向为药用植物资源及繁育。E-mail:xjlxj@126.com.
资助项目：新疆维吾尔自治区“十二五”重大专项资助项目（201130105-1）。
收稿日期：2013-06-19