[1]权永辉,刘雅莉,祁银燕,等.百合愈伤组织的诱导及瞬时表达的研究[J].北方园艺,2013,37(16):103-107.
　QUANYong-hui,LIUYa-li,QIYin-yan,et al.StudiesonCallusInductionandTransientExpressionof Liliumtenuifolium(Oriental×Trumpet)[J].Northern Horticulture,2013,37(16):103-107.
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百合愈伤组织的诱导及瞬时表达的研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年16期 页码: 103-107 栏目: 出版日期: 2013-08-30
Title:
StudiesonCallusInductionandTransientExpressionof  Liliumtenuifolium(Oriental×Trumpet)
文章编号:
1001-0009（2013）16-0103-05
作者:
权永辉1; 2; 刘雅莉1; 2; 祁银燕1; 3; 姜玲1; 2; 田菲菲1; 2; 杜灵娟1; 2
1.旱区作物逆境生物学国家重点实验室（西北农林科技大学），农业部西北地区园艺作物生物学与种质创制重点实验室，陕西杨凌712100；
2.西北农林科技大学林学院，陕西杨凌712100；3.西北农林科技大学园艺学院，陕西杨凌712100
Author(s):
QUANYong-hui 1; 2; LIUYa-li 1; 2; QIYin-yan 1; 3; JIANGLing 1; 2; TIANFei-fei 1; 2; DULing-juan 1; 2
1.StateKeyLaboratoryofCropStressBiologyinAridAreas(NorthwestAgriculturalandForestryUniversity)，KeyLaboratoryofHorticulturalPlantBiologyandGermplasmInnovationinNorthwestChina，MinistryofAgriculture，Yangling，Shaanxi712100；
2.CollegeofForestry，NorthwestAgriculturalandForestryUniversity，Yangling，Shaanxi712100；3.CollegeofHorticulture，NorthwestAgriculturalandForestryUniversity，Yangling，Shaanxi712100
关键词:
百合; 愈伤组织; 植株再生; GUS基因瞬时表达
Keywords:
lily; callus; plantregeneration; GUS genetransientexpression
分类号:
S682.2+65
文献标志码:
A
摘要:
以百合品种‘罗宾娜’（Robina）鳞片、花丝和花梗分化的不定芽为试材，诱导愈伤组织，并通过固体、液体和经过液体预培养后在固体上培养的方式继代增殖。结果表明：分化的不定芽在萘乙酸（NAA）0.5mg/L的培养基上愈伤组织的诱导率最高；从芽基部形成的愈伤组织以经过液体预培养后在固体上培养的方式继代，繁殖速率最大，再生率为100%。使用农杆菌介导转化pCAMBIAl301质粒后，3种不同方式培养的愈伤组织的GUS瞬时表达效率差异并不显著。
Abstract:
Taking LiliumtenuifoliuOriental×Tumpet‘Robina’asmaterials,andadventitiousbudswhichdifferentiatedfrombulbscales，filamentsandstalkofitwereusedtoformembryogeniccallus,multiplicationwasconductedviasolid,liquidandliquidpreculturethensolidculturemethods.Theresultsshowedthatitproducedthemostembrogeniccalluswhenexplantswereculturedoninductionmediumwhichcontained0.5mg/LNAA.Thebestresultofcallusproliferationwasmade viaaalternativeculturemethodwhichcalluswastransferredonsolidandliquidmediumbyturns.The GUSgenecontainedinvectorpCAMBIA1301wasintroducedintotheembryogenicculturesbyAgrobacterium-mediatedtracientexpressionandthenGUSchemicaltissuesstainingwasconductedtoobserve.Theresultsshowedthatitwasnosignificanttransientexpressionefficiencyvarianceamongcallusofthreecultivationmethods.

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备注/Memo

第一作者简介：权永辉（1987-），男，硕士研究生，现主要从事园林植物分子育种的研究工作。E-mail：quanyong389@sohu.com.
责任作者：刘雅莉（1960-），女，硕士，副教授，硕士生导师，研究方向为园林植物遗传育种。E-mail：lyl6151@126.com.
基金项目：国家自然科学基金资助项目(31170652)。
收稿日期：2013-04-09

更新日期/Last Update: 2014-09-12