CHEN Liwen,YANG Li-ping,RONG Yi,et al.Study on the Techniques of Industrialized Seedling Culture of Draceana arborea[J].Northern Horticulture,2013,37(13):138-141.
金心也门铁工厂化育苗技术研究
- Title:
- Study on the Techniques of Industrialized Seedling Culture of Draceana arborea
- 文章编号:
- 1001-0009(2013)13-0138-04
- 分类号:
- S 503.53
- 文献标志码:
- A
- 摘要:
- 以金心也门铁的腋芽、顶芽和叶片为外植体，研究了不定芽诱导、增殖、生根诱导及移栽管理的最佳培养条件，以期形成金心也门铁工厂化育苗技术。结果表明：适宜不定芽诱导的培养基为：改良MS+6BA 2.0 mg/L+IAA 0.5 mg/L；适宜芽继代增殖的培养基为：改良MS+6BA 1.0 mg/L+NAA 0.2 mg/L+AD 2.0 mg/L+AC 0.2%；适宜组培苗生根的培养基为1/2MS+IBA 1.0 mg/L。以腋芽或顶芽为外植体直接诱导不定芽，并用以芽繁芽的方式进行继代增殖能大大降低金心也门铁组培繁殖过程出现的变异率。光照对金心也门铁组培苗的生根有明显的影响，最适合的光照强度为2 000~3 000 lx；在此条件下培养10 d组培苗即可出根。
- Abstract:
- Taking the axillary buds，apical buds and leaves form Draceana arborea as materials，the induction，propagation，rooting and transplanting of the seedlings were studied form the techniques of industrialized seedling culture of Dracaena arborea.The results showed that the suitable medium for adventitious bud induction was reform MS+6BA 2.0 mg/L+IAA 0.5 mg/L，the suitable medium for bud proliferation was reform MS+6MS+IBA 1.0 mg/L.Induce adventitious bud with axillary buds or apical buds，and propagate by the way of bud proliferation could reduce the mutational rate of the Dracean arborea in tissue culture.Light had obvious influence with the rooting of the Draceana arborea tissue culture seedlings，the suitable light intension was 2 000~3 000 lx，the seedlings would rooting in this condition for cultured 10 days.
参考文献/References:
[1]百度百科.金心也门铁[EB/OL].http://baike.baidu.com/view/3959646.htm,2012-12-11.
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备注/Memo
第一作者简介：陈丽文（1980-），女，本科，工程师，现主要从事林木和花卉的组培技术等研究工作。